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The map label for this gene is truB [H]

Identifier: 150398629

GI number: 150398629

Start: 3650075

End: 3651007

Strand: Direct

Name: truB [H]

Synonym: Smed_3443

Alternate gene names: 150398629

Gene position: 3650075-3651007 (Clockwise)

Preceding gene: 150398628

Following gene: 150398630

Centisome position: 96.51

GC content: 63.02

Gene sequence:

>933_bases
ATGTCCAGACCCCGCAAACCAAAGGGCCGCCCGATTTCAGGATGGCTAATCCTCGACAAGCCTCTCGATTTCGGTTCGAC
CGAGGCAGTATCCAAGATCAAATGGCTGTTCAAGGCGCAGAAGGCGGGCCATGCCGGCACCCTGGATCCGCTGGCGTCCG
GAATGCTGCCCATCGCGCTCGGCGATGCAACGAAGACCGTGCCTTATGTCATGGATGGCCGCAAGATCTACGAATTCACG
GTCGCCTGGGGCGAGGAACGCTCAACGGACGACCTCGAAGGGGAGGTCGTCCGCTCCTCCGCGGACCGTCCGGCGGAAGA
GGCGATCCGGGCTCTGCTGCCCAAATATACCGGCGTGATCGCACAGGTGCCGCCGCAGTTTTCGGCGATCAAGATCGGCG
GCGAACGCGCCTATGATCTCGCACGAGAGGGTGAAACCGTCGACATCCCGGCGCGTGAGGTGGAAGTCTTTCGTCTTTCG
CTCATCGGCTCCGCGCCAAGTCTCGCACAGTTCGAAATCGAATGCGGCAAGGGCACCTATGTCCGCTCGCTGGCCCGCGA
CATGGGCCGTGATCTCGGTTGCTTCGGCCATATCGCGTCGCTGCGCCGAACGTTCGTCGCTCCCTTCGGCGAGGAGGACA
TGGTGCCGCTCGCCGATCTCGTCGCGTTGGAGAAGATCGAGGACGAGGCGGAACGTCTCGCCGCGCTGGACGAGCATCTG
ATCGATACCGGAGAGGCCTTGTCCGACCTTCCCCATATCGCCGTCAATGACGACCAGGCGCACCGGCTCCGAATGGGCAA
CCCGATCATTCTGCGCGGACGCGACGCGCCACTGCCCACGCCGGAGGCCTATGCCACCGTACAAGGCAAGCTTGTGGCGA
TCGGCGAAATCGCCGAAGGCGAATTTCGGCCGAAGCGCGTTTTCGCAGGCTAG

Upstream 100 bases:

>100_bases
CCAGCTTCGAAAACTATCAGAAGATCGACTCGCTGCTACGCTCTCCGGAGGTCAGTCGCGACCTCGATCGCGACAGTGAC
GAAGAATAAGCAACGCAACC

Downstream 100 bases:

>100_bases
CAGCGTATAACCGTTGTGCGTTGAAGCCCATCTTGTCATTCTGCCCCGCAAGCCGCGCGCGGGAAAAAGATTCGGGCGGC
AGGCTTGAGGCACACGGCAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSRPRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIALGDATKTVPYVMDGRKIYEFT
VAWGEERSTDDLEGEVVRSSADRPAEEAIRALLPKYTGVIAQVPPQFSAIKIGGERAYDLAREGETVDIPAREVEVFRLS
LIGSAPSLAQFEIECGKGTYVRSLARDMGRDLGCFGHIASLRRTFVAPFGEEDMVPLADLVALEKIEDEAERLAALDEHL
IDTGEALSDLPHIAVNDDQAHRLRMGNPIILRGRDAPLPTPEAYATVQGKLVAIGEIAEGEFRPKRVFAG

Sequences:

>Translated_310_residues
MSRPRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIALGDATKTVPYVMDGRKIYEFT
VAWGEERSTDDLEGEVVRSSADRPAEEAIRALLPKYTGVIAQVPPQFSAIKIGGERAYDLAREGETVDIPAREVEVFRLS
LIGSAPSLAQFEIECGKGTYVRSLARDMGRDLGCFGHIASLRRTFVAPFGEEDMVPLADLVALEKIEDEAERLAALDEHL
IDTGEALSDLPHIAVNDDQAHRLRMGNPIILRGRDAPLPTPEAYATVQGKLVAIGEIAEGEFRPKRVFAG
>Mature_309_residues
SRPRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIALGDATKTVPYVMDGRKIYEFTV
AWGEERSTDDLEGEVVRSSADRPAEEAIRALLPKYTGVIAQVPPQFSAIKIGGERAYDLAREGETVDIPAREVEVFRLSL
IGSAPSLAQFEIECGKGTYVRSLARDMGRDLGCFGHIASLRRTFVAPFGEEDMVPLADLVALEKIEDEAERLAALDEHLI
DTGEALSDLPHIAVNDDQAHRLRMGNPIILRGRDAPLPTPEAYATVQGKLVAIGEIAEGEFRPKRVFAG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=243, Percent_Identity=32.5102880658436, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4503337, Length=264, Percent_Identity=26.5151515151515, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI215599015, Length=264, Percent_Identity=26.5151515151515, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI2367200, Length=314, Percent_Identity=37.5796178343949, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17553978, Length=271, Percent_Identity=28.4132841328413, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=270, Percent_Identity=27.4074074074074, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=129, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281364189, Length=210, Percent_Identity=28.0952380952381, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364187, Length=210, Percent_Identity=28.0952380952381, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364185, Length=210, Percent_Identity=28.0952380952381, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364183, Length=210, Percent_Identity=28.0952380952381, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI62471759, Length=210, Percent_Identity=28.0952380952381, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17975520, Length=210, Percent_Identity=28.0952380952381, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33783; Mature: 33652

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIAL
CCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEE
GDATKTVPYVMDGRKIYEFTVAWGEERSTDDLEGEVVRSSADRPAEEAIRALLPKYTGVI
CCCCCCCCEEECCCEEEEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCE
AQVPPQFSAIKIGGERAYDLAREGETVDIPAREVEVFRLSLIGSAPSLAQFEIECGKGTY
EECCCCCEEEEECCHHHHHHHHCCCEEECCHHHEEEEEEEEECCCCCCEEEEEECCCCHH
VRSLARDMGRDLGCFGHIASLRRTFVAPFGEEDMVPLADLVALEKIEDEAERLAALDEHL
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IDTGEALSDLPHIAVNDDQAHRLRMGNPIILRGRDAPLPTPEAYATVQGKLVAIGEIAEG
HHHHHHHHHCCCEEECCCCHHHEECCCEEEEECCCCCCCCCCCEEEECCEEEEEEEECCC
EFRPKRVFAG
CCCCCCCCCC
>Mature Secondary Structure 
SRPRKPKGRPISGWLILDKPLDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIAL
CCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEE
GDATKTVPYVMDGRKIYEFTVAWGEERSTDDLEGEVVRSSADRPAEEAIRALLPKYTGVI
CCCCCCCCEEECCCEEEEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCE
AQVPPQFSAIKIGGERAYDLAREGETVDIPAREVEVFRLSLIGSAPSLAQFEIECGKGTY
EECCCCCEEEEECCHHHHHHHHCCCEEECCHHHEEEEEEEEECCCCCCEEEEEECCCCHH
VRSLARDMGRDLGCFGHIASLRRTFVAPFGEEDMVPLADLVALEKIEDEAERLAALDEHL
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IDTGEALSDLPHIAVNDDQAHRLRMGNPIILRGRDAPLPTPEAYATVQGKLVAIGEIAEG
HHHHHHHHHCCCEEECCCCHHHEECCCEEEEECCCCCCCCCCCEEEECCEEEEEEEECCC
EFRPKRVFAG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]