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The map label for this gene is glnT [H]

Identifier: 150398484

GI number: 150398484

Start: 3486709

End: 3488094

Strand: Direct

Name: glnT [H]

Synonym: Smed_3294

Alternate gene names: 150398484

Gene position: 3486709-3488094 (Clockwise)

Preceding gene: 150398483

Following gene: 150398491

Centisome position: 92.19

GC content: 59.81

Gene sequence:

>1386_bases
ATGAAGAGTGGCAGGTCGCACTTGAGAGGGTGCGGCCTGTTTTCGCATTTGAGTGTCGGAATCCAAAAGGGGAACAACGT
GACACTGGATCTCTTCACCTTCGCCCGTGACAAGGGCGTCAAATATTTCATGATCAGCTACACCGATCTCTTCGGCGGGC
AACGCGCCAAGCTCGTCCCGACGGAGGCCATTGGTGAGATGCAGAAGGGCGGCGCGGGCTTTGCCGGCTTCGCAACCTGG
TTCGATCTTACGCCTGCCCATCCGGATCTCTTCGCTCTCCCGGACCCGTCGTCGGTCATTCAGCTCCCCTGGAAAAAGGA
TGTCGCCTGGGTCGCCGCCGATTGCGTCATGGATGAGGCGCCGGTGGAACAGGCTCCGCGCGTGGTGCTTAAGAAGCTGA
TCGCCGAAGCCGCTGAGGAGGGGCTTCGCGTTAAAACCGGCGTCGAGCCGGAATTTTTCCTGATCTCGCCGGATGGTTCG
AAGATTTCCGACACGTTCGATACGGCCGAAAAGCCCTGTTACGACCAGCAGGCGATCATGCGTCGGTACGACGTGATCGC
CGAGATCTGCGATTATATGCTCGAACTCGGTTGGAAGCCCTATCAGAACGACCATGAAGACGCGAACGGCCAGTTCGAGA
TGAACTGGGAGTATGACGACGCGCTCCGTACGGCCGACAAGCACTCGTTCTTCAAGTTCATGGTGAAATCGGTCGCCGAG
AAGCACGGCTTGCGTGCGACCTTCATGCCGAAGCCGTTCAAAGGTCTGACGGGCAACGGCTGCCATTGCCATATATCGGT
CTGGGATCTGGAAGGCGAGGTGAATGCCTTTGCCGACAAGAGAGCCGAGTTCGGACTTTCCGCCGAGGGGCGCTATTTTC
TCGGCGGCATCATGAAGCATGCAAGCGCCCTCGCCGCCGTCACCAATCCGACGGTCAATTCCTACAAGCGCATCAACGCC
CCGCGCACGGTTTCCGGTGCGACCTGGGCACCAAATTCGGTGACCTGGACCGGCAACAACCGCACCCATATGGTTCGCGT
CCCCGGTCCCGGCCGTTTCGAGCTGCGCCTGCCGGACGGTGCCGTCAATCCCTATCTCCTGCAGGCGATCATCATCGCTG
CCGGCCTTTCGGGCGTTCGCTCCAAGGCTGATCCCGGCCGCCACTACGACATCGACATGTACAGGGACGGGCATCAGGTG
ACCGATGCGCCGAAGCTTCCGCTCAACCTGCTCGACGCCCTGCGGGAGTACAAGAGGGACGAAGAACTGCAGGAAGCGCT
TGGACGGGAGTTCTCAGCCGCCTATCTGAAGCTCAAGCAAGGCGAGTGGAATGCCTATTGCTCGCAGTTCACGGATTGGG
AGCACCAGACCACGCTGGACGTGTAG

Upstream 100 bases:

>100_bases
CGAGCCCGAGGACCTTTGTGCCCTGACGATGGAGGCCGCAGCGATGGCGCAGGTGCCGCTGGCGGGTACGAGTTGGTACC
CCGGCAAGGGTGCCTTCTGA

Downstream 100 bases:

>100_bases
GGCGCGTGCAGGAAAAGCGTGCCGGCTGGAGCCCTTCGGCCTGCACGTCTACTGCATGTTTCCTTTAATCGGATCCGATT
GAAGGACAAAAACATGCAGC

Product: glutamine synthetase catalytic subunit

Products: NA

Alternate protein names: Glutamate--ammonia ligase III; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKSGRSHLRGCGLFSHLSVGIQKGNNVTLDLFTFARDKGVKYFMISYTDLFGGQRAKLVPTEAIGEMQKGGAGFAGFATW
FDLTPAHPDLFALPDPSSVIQLPWKKDVAWVAADCVMDEAPVEQAPRVVLKKLIAEAAEEGLRVKTGVEPEFFLISPDGS
KISDTFDTAEKPCYDQQAIMRRYDVIAEICDYMLELGWKPYQNDHEDANGQFEMNWEYDDALRTADKHSFFKFMVKSVAE
KHGLRATFMPKPFKGLTGNGCHCHISVWDLEGEVNAFADKRAEFGLSAEGRYFLGGIMKHASALAAVTNPTVNSYKRINA
PRTVSGATWAPNSVTWTGNNRTHMVRVPGPGRFELRLPDGAVNPYLLQAIIIAAGLSGVRSKADPGRHYDIDMYRDGHQV
TDAPKLPLNLLDALREYKRDEELQEALGREFSAAYLKLKQGEWNAYCSQFTDWEHQTTLDV

Sequences:

>Translated_461_residues
MKSGRSHLRGCGLFSHLSVGIQKGNNVTLDLFTFARDKGVKYFMISYTDLFGGQRAKLVPTEAIGEMQKGGAGFAGFATW
FDLTPAHPDLFALPDPSSVIQLPWKKDVAWVAADCVMDEAPVEQAPRVVLKKLIAEAAEEGLRVKTGVEPEFFLISPDGS
KISDTFDTAEKPCYDQQAIMRRYDVIAEICDYMLELGWKPYQNDHEDANGQFEMNWEYDDALRTADKHSFFKFMVKSVAE
KHGLRATFMPKPFKGLTGNGCHCHISVWDLEGEVNAFADKRAEFGLSAEGRYFLGGIMKHASALAAVTNPTVNSYKRINA
PRTVSGATWAPNSVTWTGNNRTHMVRVPGPGRFELRLPDGAVNPYLLQAIIIAAGLSGVRSKADPGRHYDIDMYRDGHQV
TDAPKLPLNLLDALREYKRDEELQEALGREFSAAYLKLKQGEWNAYCSQFTDWEHQTTLDV
>Mature_461_residues
MKSGRSHLRGCGLFSHLSVGIQKGNNVTLDLFTFARDKGVKYFMISYTDLFGGQRAKLVPTEAIGEMQKGGAGFAGFATW
FDLTPAHPDLFALPDPSSVIQLPWKKDVAWVAADCVMDEAPVEQAPRVVLKKLIAEAAEEGLRVKTGVEPEFFLISPDGS
KISDTFDTAEKPCYDQQAIMRRYDVIAEICDYMLELGWKPYQNDHEDANGQFEMNWEYDDALRTADKHSFFKFMVKSVAE
KHGLRATFMPKPFKGLTGNGCHCHISVWDLEGEVNAFADKRAEFGLSAEGRYFLGGIMKHASALAAVTNPTVNSYKRINA
PRTVSGATWAPNSVTWTGNNRTHMVRVPGPGRFELRLPDGAVNPYLLQAIIIAAGLSGVRSKADPGRHYDIDMYRDGHQV
TDAPKLPLNLLDALREYKRDEELQEALGREFSAAYLKLKQGEWNAYCSQFTDWEHQTTLDV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=429, Percent_Identity=24.009324009324, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1790301, Length=459, Percent_Identity=30.0653594771242, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87081870, Length=453, Percent_Identity=26.9315673289183, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51445; Mature: 51445

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSGRSHLRGCGLFSHLSVGIQKGNNVTLDLFTFARDKGVKYFMISYTDLFGGQRAKLVP
CCCCHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEHHHCCCCCCEECC
TEAIGEMQKGGAGFAGFATWFDLTPAHPDLFALPDPSSVIQLPWKKDVAWVAADCVMDEA
HHHHHHHHHCCCCCCHHHHHEECCCCCCCEEECCCCCCEEECCCCCCHHHHHHHHHHCCC
PVEQAPRVVLKKLIAEAAEEGLRVKTGVEPEFFLISPDGSKISDTFDTAEKPCYDQQAIM
CHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCCCCCCCHHHHCCCCCCCHHHHH
RRYDVIAEICDYMLELGWKPYQNDHEDANGQFEMNWEYDDALRTADKHSFFKFMVKSVAE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
KHGLRATFMPKPFKGLTGNGCHCHISVWDLEGEVNAFADKRAEFGLSAEGRYFLGGIMKH
HHCCEEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
ASALAAVTNPTVNSYKRINAPRTVSGATWAPNSVTWTGNNRTHMVRVPGPGRFELRLPDG
HHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEECCCC
AVNPYLLQAIIIAAGLSGVRSKADPGRHYDIDMYRDGHQVTDAPKLPLNLLDALREYKRD
CCCHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCH
EELQEALGREFSAAYLKLKQGEWNAYCSQFTDWEHQTTLDV
HHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCEECCC
>Mature Secondary Structure
MKSGRSHLRGCGLFSHLSVGIQKGNNVTLDLFTFARDKGVKYFMISYTDLFGGQRAKLVP
CCCCHHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEHHHCCCCCCEECC
TEAIGEMQKGGAGFAGFATWFDLTPAHPDLFALPDPSSVIQLPWKKDVAWVAADCVMDEA
HHHHHHHHHCCCCCCHHHHHEECCCCCCCEEECCCCCCEEECCCCCCHHHHHHHHHHCCC
PVEQAPRVVLKKLIAEAAEEGLRVKTGVEPEFFLISPDGSKISDTFDTAEKPCYDQQAIM
CHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCCCCCCCHHHHCCCCCCCHHHHH
RRYDVIAEICDYMLELGWKPYQNDHEDANGQFEMNWEYDDALRTADKHSFFKFMVKSVAE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
KHGLRATFMPKPFKGLTGNGCHCHISVWDLEGEVNAFADKRAEFGLSAEGRYFLGGIMKH
HHCCEEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
ASALAAVTNPTVNSYKRINAPRTVSGATWAPNSVTWTGNNRTHMVRVPGPGRFELRLPDG
HHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCEEEEECCCC
AVNPYLLQAIIIAAGLSGVRSKADPGRHYDIDMYRDGHQVTDAPKLPLNLLDALREYKRD
CCCHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCH
EELQEALGREFSAAYLKLKQGEWNAYCSQFTDWEHQTTLDV
HHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]