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The map label for this gene is pckA [H]

Identifier: 150398445

GI number: 150398445

Start: 3434539

End: 3436149

Strand: Direct

Name: pckA [H]

Synonym: Smed_3253

Alternate gene names: 150398445

Gene position: 3434539-3436149 (Clockwise)

Preceding gene: 150398432

Following gene: 150398446

Centisome position: 90.82

GC content: 62.2

Gene sequence:

>1611_bases
ATGGAAGAGCTTGGCAGCCGCAACCCCTCTATAGGACTGGAATCGATCGGATTTTCTGACCTTTCGGTCGTCCGGTACAA
CTTCGAGGCGGCGCAGCTTTACGAAGAAGCTCTGGCCCATGGTGAAGCCGAATTGACGGCCCATGGCGCGCTTTGCGCCC
GTACCGGCCAGCACACAGGTCGCTCCCCAAAGGACAAATACGTCGTGCGCGACGCCAACACGGCCGACCAGATCTGGTGG
GACAACAACAGTGCCATCTCGCCGGAGAACTTCGACCGGCTGCGTCAGGACATGCTCGCGCATGCAAAAGGCATGTCGCT
CTACGTGCAGGATCTCGTCGGCGGTGCCGACCCCGAGAATGCGCTGCCCACGCGCGTCGTCACGGAGTTCGCCTGGCATT
CACTGTTCATCCGCAATCTATTGATCCGCCCCGAGCGTGAGGCACTCTCGTCTTTCCAGCCAATGCTGACGATCATCGAT
CTGCCGAGCTTCAAGGCTAGTCCTGAGCGCCACGGCTGCCGCAGCGAAACGGTCATTGCTTGCGATCTGACCAACGGTCT
GGTGCTGATCGGCGGCACCTCCTATGCGGGGGAGATGAAGAAGTCTGTCTTCACCGTGCTCAACTACCTGTTGCCCAGGA
AATCGGTGATGCCGATGCACTGCTCGGCCAATGTCGGCCCTGCCGGCGACACCGCCATCTTCTTCGGGCTCTCCGGCACC
GGCAAAACGACGCTTTCGGCTGATCCGAACCGCACTCTGATCGGTGACGACGAGCACGGCTGGGGCGAAAAGGGCGTCTT
CAATTTCGAGGGCGGCTGCTACGCCAAGGCCATCCGTCTGTCGGAAGCGGCTGAGCCTGAGATTTTCGCGACGACGCAGC
GTTTCGGGACGGTTATGGAGAACGTCGTACTCGACGAGCGGCGCATGCCGGACTTCGAAGACGGGTCTCTGACCGAAAAC
ACCCGCTGCGCCTATCCGCTTCATTTCATTCCGAATGCCAGCAAGACCGGCACGGCGCCGCAGCCGCGCACGATCATCAT
GCTGACGGCTGATGCCTTCGGCGTATTGCCGCCGATCGCAAAGCTGACGCCGGAACAGGCCATGTACCACTTCCTTTCCG
GCTACACCGCCAAGGTTGCCGGTACCGAAAAGGGCGTTACAGAGCCGGAAGCGACGTTCTCAACCTGCTTCGGCGCGCCC
TTCATGCCCCGTCATCCGTCCGAGTACGGCAATTTGCTCAAGGAACTCATCGCCCGCAACGGCGTCACCTGCTGGCTTGT
CAACACCGGCTGGACCGGCGGCGCCTATGGAACCGGAAGCCGCATGCCGATCAAGGTGACGCGCGCGCTCCTTTCGGCAG
CGCTCGACGGATCGCTGAACAGCGCCTCGTTCCGCAGCGATGCGAATTTCGGCTTCGCGGTGCCGGTATCGGTACCGGGC
GTCGAGTCCCGCATCCTCGATCCACGCTCGACCTGGGCGGACGGCGCCGCCTATGACGCGCAGGCACGCCGCCTCGTCGA
CATGTTCATCGCCAATTTCGCCAAGTTCGAGAGCCACGTGGATGGCAGCGTGCGCGATGCCGCGCCCGGTGCCAGGATAG
CCGCCGAATAA

Upstream 100 bases:

>100_bases
CATTGCCGTTTTAGAAACGCCTATCTTGTCTTAGATCTGCCTTGCCGGTATGTTCCAACGAAATTCAACGCCAATGGCCA
AACACGAAGGAAGCTTGACC

Downstream 100 bases:

>100_bases
ACCGGCCTCTTGCCTTGATTCACGTGAAACCCGGTCGCTTGCGGCCGGGTTTTGCATTTGCGGCTTGTTTTCAAGCATGC
GATAGAACGGCGGAGGAAGA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MEELGSRNPSIGLESIGFSDLSVVRYNFEAAQLYEEALAHGEAELTAHGALCARTGQHTGRSPKDKYVVRDANTADQIWW
DNNSAISPENFDRLRQDMLAHAKGMSLYVQDLVGGADPENALPTRVVTEFAWHSLFIRNLLIRPEREALSSFQPMLTIID
LPSFKASPERHGCRSETVIACDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPRKSVMPMHCSANVGPAGDTAIFFGLSGT
GKTTLSADPNRTLIGDDEHGWGEKGVFNFEGGCYAKAIRLSEAAEPEIFATTQRFGTVMENVVLDERRMPDFEDGSLTEN
TRCAYPLHFIPNASKTGTAPQPRTIIMLTADAFGVLPPIAKLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSEYGNLLKELIARNGVTCWLVNTGWTGGAYGTGSRMPIKVTRALLSAALDGSLNSASFRSDANFGFAVPVSVPG
VESRILDPRSTWADGAAYDAQARRLVDMFIANFAKFESHVDGSVRDAAPGARIAAE

Sequences:

>Translated_536_residues
MEELGSRNPSIGLESIGFSDLSVVRYNFEAAQLYEEALAHGEAELTAHGALCARTGQHTGRSPKDKYVVRDANTADQIWW
DNNSAISPENFDRLRQDMLAHAKGMSLYVQDLVGGADPENALPTRVVTEFAWHSLFIRNLLIRPEREALSSFQPMLTIID
LPSFKASPERHGCRSETVIACDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPRKSVMPMHCSANVGPAGDTAIFFGLSGT
GKTTLSADPNRTLIGDDEHGWGEKGVFNFEGGCYAKAIRLSEAAEPEIFATTQRFGTVMENVVLDERRMPDFEDGSLTEN
TRCAYPLHFIPNASKTGTAPQPRTIIMLTADAFGVLPPIAKLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSEYGNLLKELIARNGVTCWLVNTGWTGGAYGTGSRMPIKVTRALLSAALDGSLNSASFRSDANFGFAVPVSVPG
VESRILDPRSTWADGAAYDAQARRLVDMFIANFAKFESHVDGSVRDAAPGARIAAE
>Mature_536_residues
MEELGSRNPSIGLESIGFSDLSVVRYNFEAAQLYEEALAHGEAELTAHGALCARTGQHTGRSPKDKYVVRDANTADQIWW
DNNSAISPENFDRLRQDMLAHAKGMSLYVQDLVGGADPENALPTRVVTEFAWHSLFIRNLLIRPEREALSSFQPMLTIID
LPSFKASPERHGCRSETVIACDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPRKSVMPMHCSANVGPAGDTAIFFGLSGT
GKTTLSADPNRTLIGDDEHGWGEKGVFNFEGGCYAKAIRLSEAAEPEIFATTQRFGTVMENVVLDERRMPDFEDGSLTEN
TRCAYPLHFIPNASKTGTAPQPRTIIMLTADAFGVLPPIAKLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSEYGNLLKELIARNGVTCWLVNTGWTGGAYGTGSRMPIKVTRALLSAALDGSLNSASFRSDANFGFAVPVSVPG
VESRILDPRSTWADGAAYDAQARRLVDMFIANFAKFESHVDGSVRDAAPGARIAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=522, Percent_Identity=47.7011494252874, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322950, Length=526, Percent_Identity=44.8669201520913, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58094; Mature: 58094

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELGSRNPSIGLESIGFSDLSVVRYNFEAAQLYEEALAHGEAELTAHGALCARTGQHTG
CCCCCCCCCCCCHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCC
RSPKDKYVVRDANTADQIWWDNNSAISPENFDRLRQDMLAHAKGMSLYVQDLVGGADPEN
CCCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCC
ALPTRVVTEFAWHSLFIRNLLIRPEREALSSFQPMLTIIDLPSFKASPERHGCRSETVIA
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEEECCCCCCCCHHCCCCCCCEEE
CDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPRKSVMPMHCSANVGPAGDTAIFFGLSGT
EECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEEEECCC
GKTTLSADPNRTLIGDDEHGWGEKGVFNFEGGCYAKAIRLSEAAEPEIFATTQRFGTVME
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCEEEHHHHHHHHHH
NVVLDERRMPDFEDGSLTENTRCAYPLHFIPNASKTGTAPQPRTIIMLTADAFGVLPPIA
HHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
KLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLKELIARN
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GVTCWLVNTGWTGGAYGTGSRMPIKVTRALLSAALDGSLNSASFRSDANFGFAVPVSVPG
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCC
VESRILDPRSTWADGAAYDAQARRLVDMFIANFAKFESHVDGSVRDAAPGARIAAE
CHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEELGSRNPSIGLESIGFSDLSVVRYNFEAAQLYEEALAHGEAELTAHGALCARTGQHTG
CCCCCCCCCCCCHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCEEECCHHHHHCCCCCC
RSPKDKYVVRDANTADQIWWDNNSAISPENFDRLRQDMLAHAKGMSLYVQDLVGGADPEN
CCCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCC
ALPTRVVTEFAWHSLFIRNLLIRPEREALSSFQPMLTIIDLPSFKASPERHGCRSETVIA
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEEECCCCCCCCHHCCCCCCCEEE
CDLTNGLVLIGGTSYAGEMKKSVFTVLNYLLPRKSVMPMHCSANVGPAGDTAIFFGLSGT
EECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEEEECCC
GKTTLSADPNRTLIGDDEHGWGEKGVFNFEGGCYAKAIRLSEAAEPEIFATTQRFGTVME
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCEEEHHHHHHHHHH
NVVLDERRMPDFEDGSLTENTRCAYPLHFIPNASKTGTAPQPRTIIMLTADAFGVLPPIA
HHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
KLTPEQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSEYGNLLKELIARN
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
GVTCWLVNTGWTGGAYGTGSRMPIKVTRALLSAALDGSLNSASFRSDANFGFAVPVSVPG
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCC
VESRILDPRSTWADGAAYDAQARRLVDMFIANFAKFESHVDGSVRDAAPGARIAAE
CHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883700; 11481430 [H]