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The map label for this gene is folC [H]

Identifier: 150398432

GI number: 150398432

Start: 3414978

End: 3416321

Strand: Direct

Name: folC [H]

Synonym: Smed_3240

Alternate gene names: 150398432

Gene position: 3414978-3416321 (Clockwise)

Preceding gene: 150398431

Following gene: 150398445

Centisome position: 90.3

GC content: 64.88

Gene sequence:

>1344_bases
ATGACGGCGACTGAGGTCAGCGAGGCGGCGCGGGAGATCGAGAAGCTTCTCGCCCTCCACCCGAAAGGATTCGACCTCTC
GCTCGACAGGATCACGCGGCTGCTCGCCGCTCTCGGCAATCCACAAGAGCGCCTGCCCCCGGTCATTCACGTCGCGGGGA
CGAACGGCAAGGGCTCGGTCACGGCCTTCTGCCGTTCGATTCTGGAGGCTGCGGGCCTGAGCGTCCACGTTCACACATCG
CCGCATCTGGTGAACTGGCACGAGCGCTACCGCCTGGGCGTCAAGGACGGCAAGGGCGCTTTGGTCAGCGACCCTGTGCT
CGCAGACGCCGTGCGGCGCGTCGCAGAAGCGAATGGCGGCCAGAAGATTACCGTCTTTGAAATCCTGACCGCCGTCACTT
TCCTTCTTTTCGCGGAGCATCCGGCCGATGTCGCGATCATAGAAGTCGGTCTCGGCGGCCGATATGACGCGACCAATGTC
ATCGCTCGGCCGGCGGTCTCGGTGATCATGCCCATTTCGCTCGACCATCAGGCATATCTCGGCGATCGAGTCGAACTGAT
CGCTGCGGAAAAGGCCGGGATCATGAAGCGCGGCTGCCCGGTGGTGATCGGCCATCAGGAGGAAGAAAGCGCACGCGAGG
TGCTGATCGAGACAGCCGAACGCCTTGGCTGCCCCACATCCGTGTACGGGCAGGATTTCATGGCGCATGAGGAGTTTGGC
AGGCTGATCTTCCAGGACGAGAACGGCCTGGCCGATTTGCCGTTGCCCCGCCTGCCGGGCAGGCATCAATATGCCAATGC
CGCTGCTGCCATCCGCGCCGTACGGGCAGCCGGCTTCGATGTTCCGGAGCCAGCGATCGAGAAGGGACTGGCCGGCGTCG
AGTGGCCAGGGCGGCTGCAGCGTCTCGCGGGCGGCAGGCTTTCGCATTTCGGCCCGCCGGGCGCCGAGATCTGGATCGAC
GGCGGGCATAATCCCGGTGCCGGCCATGTCATCGCCGAGGCGATGGCCACCTTCGAGGAGCGTGACCCGAGGCCGCTCTT
TCTGATCATCGGGATGATCAACACCAAGGATCCGATCGGTTATTTCAAGGCTTTTCTCGACCTGACCGAACAGGTCTTCA
CCGTGCCTGTTCACGGTTCCGATGCAGGGCTGGACCCTCTGGCGCTCGCTGCGGACGCGGCCGCTGCCGGGCTCGAGGCC
AATGCCGCTTCCTCGGTCGCCGAAGCGCTGGGCCAGATCGCCGAGCTCACGGACGAGGACCCGGTGCCACCGCGTGTGCT
GATCGGCGGATCGCTCTATCTGGTGGGCGACGTGCTGGCGGAGAACGGTACGCCGCCGCGATAG

Upstream 100 bases:

>100_bases
GGAGTGCTTGATTTAAAGTGTGAGATCCGGATGCGCGCCTGCGCGCGCATCCCATCTGTCCTCATCCTGCTCCGGCGGGG
TCGTTTTCGGGGCTACCATC

Downstream 100 bases:

>100_bases
GTTCTACCTGACAATCGCCCTCCCTCCGTTCACAGGAAGATATGCGGATGAGCGCGCCCTGGTCTTATCATGTGCGCCTG
CCCGAAAGAAAAAGCCCGGC

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTATEVSEAAREIEKLLALHPKGFDLSLDRITRLLAALGNPQERLPPVIHVAGTNGKGSVTAFCRSILEAAGLSVHVHTS
PHLVNWHERYRLGVKDGKGALVSDPVLADAVRRVAEANGGQKITVFEILTAVTFLLFAEHPADVAIIEVGLGGRYDATNV
IARPAVSVIMPISLDHQAYLGDRVELIAAEKAGIMKRGCPVVIGHQEEESAREVLIETAERLGCPTSVYGQDFMAHEEFG
RLIFQDENGLADLPLPRLPGRHQYANAAAAIRAVRAAGFDVPEPAIEKGLAGVEWPGRLQRLAGGRLSHFGPPGAEIWID
GGHNPGAGHVIAEAMATFEERDPRPLFLIIGMINTKDPIGYFKAFLDLTEQVFTVPVHGSDAGLDPLALAADAAAAGLEA
NAASSVAEALGQIAELTDEDPVPPRVLIGGSLYLVGDVLAENGTPPR

Sequences:

>Translated_447_residues
MTATEVSEAAREIEKLLALHPKGFDLSLDRITRLLAALGNPQERLPPVIHVAGTNGKGSVTAFCRSILEAAGLSVHVHTS
PHLVNWHERYRLGVKDGKGALVSDPVLADAVRRVAEANGGQKITVFEILTAVTFLLFAEHPADVAIIEVGLGGRYDATNV
IARPAVSVIMPISLDHQAYLGDRVELIAAEKAGIMKRGCPVVIGHQEEESAREVLIETAERLGCPTSVYGQDFMAHEEFG
RLIFQDENGLADLPLPRLPGRHQYANAAAAIRAVRAAGFDVPEPAIEKGLAGVEWPGRLQRLAGGRLSHFGPPGAEIWID
GGHNPGAGHVIAEAMATFEERDPRPLFLIIGMINTKDPIGYFKAFLDLTEQVFTVPVHGSDAGLDPLALAADAAAAGLEA
NAASSVAEALGQIAELTDEDPVPPRVLIGGSLYLVGDVLAENGTPPR
>Mature_446_residues
TATEVSEAAREIEKLLALHPKGFDLSLDRITRLLAALGNPQERLPPVIHVAGTNGKGSVTAFCRSILEAAGLSVHVHTSP
HLVNWHERYRLGVKDGKGALVSDPVLADAVRRVAEANGGQKITVFEILTAVTFLLFAEHPADVAIIEVGLGGRYDATNVI
ARPAVSVIMPISLDHQAYLGDRVELIAAEKAGIMKRGCPVVIGHQEEESAREVLIETAERLGCPTSVYGQDFMAHEEFGR
LIFQDENGLADLPLPRLPGRHQYANAAAAIRAVRAAGFDVPEPAIEKGLAGVEWPGRLQRLAGGRLSHFGPPGAEIWIDG
GHNPGAGHVIAEAMATFEERDPRPLFLIIGMINTKDPIGYFKAFLDLTEQVFTVPVHGSDAGLDPLALAADAAAAGLEAN
AASSVAEALGQIAELTDEDPVPPRVLIGGSLYLVGDVLAENGTPPR

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=351, Percent_Identity=29.0598290598291, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI66932990, Length=351, Percent_Identity=29.0598290598291, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1788654, Length=441, Percent_Identity=32.1995464852608, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17553150, Length=482, Percent_Identity=28.2157676348548, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71984923, Length=482, Percent_Identity=28.2157676348548, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=482, Percent_Identity=28.2157676348548, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323760, Length=436, Percent_Identity=30.5045871559633, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324815, Length=448, Percent_Identity=25.2232142857143, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322718, Length=295, Percent_Identity=26.1016949152542, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24641571, Length=321, Percent_Identity=25.8566978193146, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=32.4022346368715, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47454; Mature: 47322

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATEVSEAAREIEKLLALHPKGFDLSLDRITRLLAALGNPQERLPPVIHVAGTNGKGSV
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHH
TAFCRSILEAAGLSVHVHTSPHLVNWHERYRLGVKDGKGALVSDPVLADAVRRVAEANGG
HHHHHHHHHHCCCEEEEECCCCEECHHHHHEECCCCCCCCEECCHHHHHHHHHHHHCCCC
QKITVFEILTAVTFLLFAEHPADVAIIEVGLGGRYDATNVIARPAVSVIMPISLDHQAYL
CEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHCHHHEEEEECCCCHHHC
GDRVELIAAEKAGIMKRGCPVVIGHQEEESAREVLIETAERLGCPTSVYGQDFMAHEEFG
CCCEEEEEHHHHCHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHC
RLIFQDENGLADLPLPRLPGRHQYANAAAAIRAVRAAGFDVPEPAIEKGLAGVEWPGRLQ
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHH
RLAGGRLSHFGPPGAEIWIDGGHNPGAGHVIAEAMATFEERDPRPLFLIIGMINTKDPIG
HHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHH
YFKAFLDLTEQVFTVPVHGSDAGLDPLALAADAAAAGLEANAASSVAEALGQIAELTDED
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
PVPPRVLIGGSLYLVGDVLAENGTPPR
CCCCEEEECCCEEEEHHHHHCCCCCCC
>Mature Secondary Structure 
TATEVSEAAREIEKLLALHPKGFDLSLDRITRLLAALGNPQERLPPVIHVAGTNGKGSV
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHH
TAFCRSILEAAGLSVHVHTSPHLVNWHERYRLGVKDGKGALVSDPVLADAVRRVAEANGG
HHHHHHHHHHCCCEEEEECCCCEECHHHHHEECCCCCCCCEECCHHHHHHHHHHHHCCCC
QKITVFEILTAVTFLLFAEHPADVAIIEVGLGGRYDATNVIARPAVSVIMPISLDHQAYL
CEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHCHHHEEEEECCCCHHHC
GDRVELIAAEKAGIMKRGCPVVIGHQEEESAREVLIETAERLGCPTSVYGQDFMAHEEFG
CCCEEEEEHHHHCHHHCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHC
RLIFQDENGLADLPLPRLPGRHQYANAAAAIRAVRAAGFDVPEPAIEKGLAGVEWPGRLQ
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHH
RLAGGRLSHFGPPGAEIWIDGGHNPGAGHVIAEAMATFEERDPRPLFLIIGMINTKDPIG
HHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHH
YFKAFLDLTEQVFTVPVHGSDAGLDPLALAADAAAAGLEANAASSVAEALGQIAELTDED
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
PVPPRVLIGGSLYLVGDVLAENGTPPR
CCCCEEEECCCEEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]