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The map label for this gene is hpcH [H]

Identifier: 150398415

GI number: 150398415

Start: 3398032

End: 3398832

Strand: Direct

Name: hpcH [H]

Synonym: Smed_3223

Alternate gene names: 150398415

Gene position: 3398032-3398832 (Clockwise)

Preceding gene: 150398414

Following gene: 150398417

Centisome position: 89.85

GC content: 65.79

Gene sequence:

>801_bases
ATGCCCGCCCCGAAAAATACCTTCAAAGCGGCGCTCCGCGAAAACCGCTTCCAACTCGGTCTCTGGGTGGCGCTCGCCAG
TCCCTATGCTGCCGAAGTGGTGGCCGGCAGTGGATATGACTGGCTGCTGATCGATGGCGAGCATGCGCCGAACGACCTTC
CGATGCTGGCGGCGCAGTATCGCGCCATTGCGGGCGGGGGCAGCCATCCGATCGTGCGCCTGCCGGTCGGCGACGCGGTC
ATGATCAAGCAGATCCTCGATGCCGGCGTTCAGACCGTGCTGATCCCGATGGTCGACGATGTCGAGCAGGCGCGCCAGCT
CGTGCGCGCTGTCCGCTATCCGCCTCACGGCATTCGAGGCGTCGGTGCCGCACTGGGGCGGGCCACGAATTTCAGCCGGA
TCGGCGACTATCTTGAGAACGCCAATGACGAGATCTGCCTGCTTCTGCAGATAGAGAGCCGGGCAGGGCTCGAGGCGATC
GACGCCATTGCCGCGCTTGACGGCGTCGACGGCCTGTTCGTCGGCCCGGCAGATCTTGCCGCTGATATGGGTTATCTCGG
CAACCCCGGCCATCCGGAGGTGCGAACCGCGATCATCGACGCTTTTGAGCGCATTCGGCAGGCGGGCAAGGCGCGCGGCA
TCATGACCCTCGATCCGGCCCAGGCGCGCGATTATCGCGACCTCGGTGCCGATTTCATGGCCATCGGCACCGACGTGACG
CTGCTCGTCAGCGCCTCGGAGCGGCTGCGCCTGGAGTTCCTTGGAGAAGCTGGACCGGCCCGGCGCGAAGCCGGCTACTG
A

Upstream 100 bases:

>100_bases
TTCTGGCCGGCTTTTTCTTTTGTCGGTAAGAGGTTACGAACTCACGTTTTTGCCGGCGGATTACGGCAGACGGACTCGGA
CGCGAAGAGGAGAGCAATCG

Downstream 100 bases:

>100_bases
TCACGGGATCGATTGCAGCAGTGTCTCCCGCGCCGACTTCAGATGCCGCCGGCAGGCCTGCTCGGCCGACATGGGATCCC
GCGACTGCAGCGCAGCGATA

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPAPKNTFKAALRENRFQLGLWVALASPYAAEVVAGSGYDWLLIDGEHAPNDLPMLAAQYRAIAGGGSHPIVRLPVGDAV
MIKQILDAGVQTVLIPMVDDVEQARQLVRAVRYPPHGIRGVGAALGRATNFSRIGDYLENANDEICLLLQIESRAGLEAI
DAIAALDGVDGLFVGPADLAADMGYLGNPGHPEVRTAIIDAFERIRQAGKARGIMTLDPAQARDYRDLGADFMAIGTDVT
LLVSASERLRLEFLGEAGPARREAGY

Sequences:

>Translated_266_residues
MPAPKNTFKAALRENRFQLGLWVALASPYAAEVVAGSGYDWLLIDGEHAPNDLPMLAAQYRAIAGGGSHPIVRLPVGDAV
MIKQILDAGVQTVLIPMVDDVEQARQLVRAVRYPPHGIRGVGAALGRATNFSRIGDYLENANDEICLLLQIESRAGLEAI
DAIAALDGVDGLFVGPADLAADMGYLGNPGHPEVRTAIIDAFERIRQAGKARGIMTLDPAQARDYRDLGADFMAIGTDVT
LLVSASERLRLEFLGEAGPARREAGY
>Mature_265_residues
PAPKNTFKAALRENRFQLGLWVALASPYAAEVVAGSGYDWLLIDGEHAPNDLPMLAAQYRAIAGGGSHPIVRLPVGDAVM
IKQILDAGVQTVLIPMVDDVEQARQLVRAVRYPPHGIRGVGAALGRATNFSRIGDYLENANDEICLLLQIESRAGLEAID
AIAALDGVDGLFVGPADLAADMGYLGNPGHPEVRTAIIDAFERIRQAGKARGIMTLDPAQARDYRDLGADFMAIGTDVTL
LVSASERLRLEFLGEAGPARREAGY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=256, Percent_Identity=48.828125, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=46.3709677419355, Blast_Score=235, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28426; Mature: 28295

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPKNTFKAALRENRFQLGLWVALASPYAAEVVAGSGYDWLLIDGEHAPNDLPMLAAQY
CCCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHEECCCCEEEEECCCCCCCCCHHHHHHH
RAIAGGGSHPIVRLPVGDAVMIKQILDAGVQTVLIPMVDDVEQARQLVRAVRYPPHGIRG
HHHCCCCCCCEEEECCCCHHHHHHHHHCCHHEEEEHHHCCHHHHHHHHHHHCCCCCCCHH
VGAALGRATNFSRIGDYLENANDEICLLLQIESRAGLEAIDAIAALDGVDGLFVGPADLA
HHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEECHHHHH
ADMGYLGNPGHPEVRTAIIDAFERIRQAGKARGIMTLDPAQARDYRDLGADFMAIGTDVT
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCHHHHCCCCEEECCCEE
LLVSASERLRLEFLGEAGPARREAGY
EEEECCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
PAPKNTFKAALRENRFQLGLWVALASPYAAEVVAGSGYDWLLIDGEHAPNDLPMLAAQY
CCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHEECCCCEEEEECCCCCCCCCHHHHHHH
RAIAGGGSHPIVRLPVGDAVMIKQILDAGVQTVLIPMVDDVEQARQLVRAVRYPPHGIRG
HHHCCCCCCCEEEECCCCHHHHHHHHHCCHHEEEEHHHCCHHHHHHHHHHHCCCCCCCHH
VGAALGRATNFSRIGDYLENANDEICLLLQIESRAGLEAIDAIAALDGVDGLFVGPADLA
HHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEECHHHHH
ADMGYLGNPGHPEVRTAIIDAFERIRQAGKARGIMTLDPAQARDYRDLGADFMAIGTDVT
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCHHHHCCCCEEECCCEE
LLVSASERLRLEFLGEAGPARREAGY
EEEECCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA