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The map label for this gene is dnaQ [H]

Identifier: 150398405

GI number: 150398405

Start: 3388060

End: 3388788

Strand: Direct

Name: dnaQ [H]

Synonym: Smed_3213

Alternate gene names: 150398405

Gene position: 3388060-3388788 (Clockwise)

Preceding gene: 150398404

Following gene: 150398408

Centisome position: 89.59

GC content: 60.49

Gene sequence:

>729_bases
ATGCGTGAAATCATCTTCGATACGGAAACGACCGGTCTCGACAACCGCGAGGACCGGGTCATCGAGATCGGCGGAATCGA
GCTCGAGAATCAGTTTCCGACGGGGCGCACGATCCATATCTATATCAATCCGGGCGATCGCAAGGTTCATCCGGAAGCGC
TCGCCGTCCACGGCATTACGGATGATTTCCTGAAGGACAAGCCGTCCTTCGCCGAGATAGCACAGGAAATCGTCGATTTC
TTCGGCGATGCCCGCTGGGTCGCCCACAACGCGACCTTCGATATGGGCTTCATCAATGCGGAGTTTGAGCGGCTCGGTCT
GCCGCCGGTTGGCAGCGACAGGGTGATCGACACGCTATCCCTTGCCCGGCGCAAGCACCCGATGGGGCCGAATTCGCTCG
ATGCGCTCTGCCGCCGCTACGGTATCGACAACTCCCATCGAACCAGGCACGGGGCGCTGCTCGACTCCGAACTCCTCGCC
GAAGTCTATATTGAGATGATCGGGGGGCGCCAGGCGGCGCTGGGTCTCGTGATGACGGAGACGGGTGGACGGTCGCTCGA
AGCCGACGACGGGCCGGTTATCATCGTCGGGAGGGAGCGGCCTTTGCGGCCACGGCTGACGGAGGCCGAAATCGCTGCTC
ATGCGGCGCTCGTCTCGAAGATCGGCGCAAATGCAATATGGGCAAAATTCAGCGAAGCTGAAGATATCCTGAGATCGGAA
GCCGTTTAG

Upstream 100 bases:

>100_bases
CAAAGCCTTCGCCGCAACTTCAATTGACGGTCGAATGACACTAAGAATGGAAAACAGCTTCGGGTGATTCGCTCCGAAAC
ATCGTCTACAGGGCTCCCGC

Downstream 100 bases:

>100_bases
CGCCTACGGCGCCGTGCAGACGCACAAAGGCCGCTGTAGCACCTTGATCTACTGCCCGTGGCAAAACGGTGGAGCACCGT
TCCTATTTGCGAACTGGTTG

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MREIIFDTETTGLDNREDRVIEIGGIELENQFPTGRTIHIYINPGDRKVHPEALAVHGITDDFLKDKPSFAEIAQEIVDF
FGDARWVAHNATFDMGFINAEFERLGLPPVGSDRVIDTLSLARRKHPMGPNSLDALCRRYGIDNSHRTRHGALLDSELLA
EVYIEMIGGRQAALGLVMTETGGRSLEADDGPVIIVGRERPLRPRLTEAEIAAHAALVSKIGANAIWAKFSEAEDILRSE
AV

Sequences:

>Translated_242_residues
MREIIFDTETTGLDNREDRVIEIGGIELENQFPTGRTIHIYINPGDRKVHPEALAVHGITDDFLKDKPSFAEIAQEIVDF
FGDARWVAHNATFDMGFINAEFERLGLPPVGSDRVIDTLSLARRKHPMGPNSLDALCRRYGIDNSHRTRHGALLDSELLA
EVYIEMIGGRQAALGLVMTETGGRSLEADDGPVIIVGRERPLRPRLTEAEIAAHAALVSKIGANAIWAKFSEAEDILRSE
AV
>Mature_242_residues
MREIIFDTETTGLDNREDRVIEIGGIELENQFPTGRTIHIYINPGDRKVHPEALAVHGITDDFLKDKPSFAEIAQEIVDF
FGDARWVAHNATFDMGFINAEFERLGLPPVGSDRVIDTLSLARRKHPMGPNSLDALCRRYGIDNSHRTRHGALLDSELLA
EVYIEMIGGRQAALGLVMTETGGRSLEADDGPVIIVGRERPLRPRLTEAEIAAHAALVSKIGANAIWAKFSEAEDILRSE
AV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786409, Length=238, Percent_Identity=45.7983193277311, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI1787941, Length=181, Percent_Identity=29.8342541436464, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006054
- InterPro:   IPR006309
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 26749; Mature: 26749

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIIFDTETTGLDNREDRVIEIGGIELENQFPTGRTIHIYINPGDRKVHPEALAVHGIT
CCCEEECCCCCCCCCCCCCEEEECCEEEECCCCCCCEEEEEECCCCCEECCCCEEEECCC
DDFLKDKPSFAEIAQEIVDFFGDARWVAHNATFDMGFINAEFERLGLPPVGSDRVIDTLS
HHHHHCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEEECCHHHHCCCCCCCCCHHHHHHH
LARRKHPMGPNSLDALCRRYGIDNSHRTRHGALLDSELLAEVYIEMIGGRQAALGLVMTE
HHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEEC
TGGRSLEADDGPVIIVGRERPLRPRLTEAEIAAHAALVSKIGANAIWAKFSEAEDILRSE
CCCCEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
AV
CC
>Mature Secondary Structure
MREIIFDTETTGLDNREDRVIEIGGIELENQFPTGRTIHIYINPGDRKVHPEALAVHGIT
CCCEEECCCCCCCCCCCCCEEEECCEEEECCCCCCCEEEEEECCCCCEECCCCEEEECCC
DDFLKDKPSFAEIAQEIVDFFGDARWVAHNATFDMGFINAEFERLGLPPVGSDRVIDTLS
HHHHHCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEEECCHHHHCCCCCCCCCHHHHHHH
LARRKHPMGPNSLDALCRRYGIDNSHRTRHGALLDSELLAEVYIEMIGGRQAALGLVMTE
HHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEEC
TGGRSLEADDGPVIIVGRERPLRPRLTEAEIAAHAALVSKIGANAIWAKFSEAEDILRSE
CCCCEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
AV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3023634; 6316347; 3540531; 9278503; 1575709 [H]