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The map label for this gene is amiC [H]

Identifier: 150396180

GI number: 150396180

Start: 1034795

End: 1036045

Strand: Direct

Name: amiC [H]

Synonym: Smed_0959

Alternate gene names: 150396180

Gene position: 1034795-1036045 (Clockwise)

Preceding gene: 150396179

Following gene: 150396181

Centisome position: 27.36

GC content: 61.47

Gene sequence:

>1251_bases
GTGAAGCGTTCGGCAAATCAGCTGTATTCCATGCGGATGATCGCCCGCCATTGCGGTTTGCGCAGGCTGGTCGCTGCCGT
TTTGTTTCTCCTGCTGCCGGTCGTGGGCGTGCACGCCGGCGAGCCGGTGCCTCTCCTTGCCTACGCGGCGCGGATCGCCG
GCGATGATGCCCGCACGCGGCTGGTGATCGAGTTCGATCGCGAACCGGTCTTTTCGGTCCACTACGTAGCCAATCCCGTC
CGCATCATCGTGGACCTGCCGGAAACCTCCTTCGGGCTTAAGCCCGAGAGCCTGGAGCCCCGCGGGCTGTTCGACGCCAT
CCGCTATGGAGGAATGGGCGCAGGGGCCTCACGGCTTGTGCTCTCCGCCAAGGAGCCGACGGAATTGACTCATGCGGAAG
TGAAGCCGATGGAGGATGGAAAGGGATTTCGCCTCGTGCTCGACGCCGAGAGGATAGGCCAGGACCGCTTCGACAAGATC
CTCGCAGAGCAGAAGTGGACGGGCACGGTGCGCGCCGTCAAAACCGACAGGCCGGCCGCAGCACCCGCCAAGGCTGATGC
TTTCGTGATTGCCATAGACGCCGGCCATGGCGGCATCGATACCGGCGCAATCGGCAGCGTCACGAAAACCGAAGAGAAAC
ACGTCACACTGGCCTTCGCAAAAGAACTGGTGGAAACGCTCAATCGAGAGCCTGGCATCGAAGCGTTCCTGACGCGGGAA
CGCGACGAGTTTCTTTCCTTGCCTCAACGCGTGCAGATCGCGCGTCAGCGAGGATCCAGCCTGTTCATTTCCCTGCATGC
CGACACTTTGAAGCAAAGGGACATTCGAGGTGCAACGGTCTACACGATTTCCGACAAGGCGTCCGATCACCTTGCCGCCG
ATCTCGCCGCGCGCGAAAACCTGTCCGACGAGATCGCCGGGATACCGCTTGAAAGCGAACCGGCCGAAGTCGCCGACATC
CTCATCGATCTCACCCGTCGCGAAACGCAGGCCTTTTCGGTCAATCTCGCGCGAAGCGTCATCGCTTCGTTCGAAGGGCA
GATCAAGCTGATCAACAACCCGCACCGCCATGCGGGATTTCGGGTCCTGCAGGCACCGGACGTGCCCTCGGTGCTGCTCG
AGCTCGGCTTCATGTCCAACAAGGAAGATGAGAAGCAACTGCTCGATCCGGAATGGCGAAAGAAGGTGTCCGCGCTGCTT
GCCGCCGCCGTACAGAGCTATCGAAACACCGCGGTTGCAAACGGCGGTTGA

Upstream 100 bases:

>100_bases
CAAGCTTTGGTTAACCCTGAGGGTTCATTGGTCGAAGAGAAGGTAAGCTGTGCCGGAGAGCCCTTTGCCGGTACTTTGAA
CGAAAGGATGCTGGGTTCAA

Downstream 100 bases:

>100_bases
AGGCGCACGGTGTTTTTAGCCGCCGATCCGATCCCGTGCCGGAAGGGCCTGAGATCATGAATATGACGGATACAATACGT
TAGAGCACGTTGGGATGAAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKRSANQLYSMRMIARHCGLRRLVAAVLFLLLPVVGVHAGEPVPLLAYAARIAGDDARTRLVIEFDREPVFSVHYVANPV
RIIVDLPETSFGLKPESLEPRGLFDAIRYGGMGAGASRLVLSAKEPTELTHAEVKPMEDGKGFRLVLDAERIGQDRFDKI
LAEQKWTGTVRAVKTDRPAAAPAKADAFVIAIDAGHGGIDTGAIGSVTKTEEKHVTLAFAKELVETLNREPGIEAFLTRE
RDEFLSLPQRVQIARQRGSSLFISLHADTLKQRDIRGATVYTISDKASDHLAADLAARENLSDEIAGIPLESEPAEVADI
LIDLTRRETQAFSVNLARSVIASFEGQIKLINNPHRHAGFRVLQAPDVPSVLLELGFMSNKEDEKQLLDPEWRKKVSALL
AAAVQSYRNTAVANGG

Sequences:

>Translated_416_residues
MKRSANQLYSMRMIARHCGLRRLVAAVLFLLLPVVGVHAGEPVPLLAYAARIAGDDARTRLVIEFDREPVFSVHYVANPV
RIIVDLPETSFGLKPESLEPRGLFDAIRYGGMGAGASRLVLSAKEPTELTHAEVKPMEDGKGFRLVLDAERIGQDRFDKI
LAEQKWTGTVRAVKTDRPAAAPAKADAFVIAIDAGHGGIDTGAIGSVTKTEEKHVTLAFAKELVETLNREPGIEAFLTRE
RDEFLSLPQRVQIARQRGSSLFISLHADTLKQRDIRGATVYTISDKASDHLAADLAARENLSDEIAGIPLESEPAEVADI
LIDLTRRETQAFSVNLARSVIASFEGQIKLINNPHRHAGFRVLQAPDVPSVLLELGFMSNKEDEKQLLDPEWRKKVSALL
AAAVQSYRNTAVANGG
>Mature_416_residues
MKRSANQLYSMRMIARHCGLRRLVAAVLFLLLPVVGVHAGEPVPLLAYAARIAGDDARTRLVIEFDREPVFSVHYVANPV
RIIVDLPETSFGLKPESLEPRGLFDAIRYGGMGAGASRLVLSAKEPTELTHAEVKPMEDGKGFRLVLDAERIGQDRFDKI
LAEQKWTGTVRAVKTDRPAAAPAKADAFVIAIDAGHGGIDTGAIGSVTKTEEKHVTLAFAKELVETLNREPGIEAFLTRE
RDEFLSLPQRVQIARQRGSSLFISLHADTLKQRDIRGATVYTISDKASDHLAADLAARENLSDEIAGIPLESEPAEVADI
LIDLTRRETQAFSVNLARSVIASFEGQIKLINNPHRHAGFRVLQAPDVPSVLLELGFMSNKEDEKQLLDPEWRKKVSALL
AAAVQSYRNTAVANGG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=374, Percent_Identity=31.0160427807487, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1788776, Length=220, Percent_Identity=40, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1790611, Length=429, Percent_Identity=27.2727272727273, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45502; Mature: 45502

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSANQLYSMRMIARHCGLRRLVAAVLFLLLPVVGVHAGEPVPLLAYAARIAGDDARTR
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEE
LVIEFDREPVFSVHYVANPVRIIVDLPETSFGLKPESLEPRGLFDAIRYGGMGAGASRLV
EEEEECCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHEE
LSAKEPTELTHAEVKPMEDGKGFRLVLDAERIGQDRFDKILAEQKWTGTVRAVKTDRPAA
EECCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCHHHHHHHHHCCCCCCEEEEEECCCCCC
APAKADAFVIAIDAGHGGIDTGAIGSVTKTEEKHVTLAFAKELVETLNREPGIEAFLTRE
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCC
RDEFLSLPQRVQIARQRGSSLFISLHADTLKQRDIRGATVYTISDKASDHLAADLAAREN
HHHHHCCHHHHHHHHHCCCEEEEEEEHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCC
LSDEIAGIPLESEPAEVADILIDLTRRETQAFSVNLARSVIASFEGQIKLINNPHRHAGF
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCE
RVLQAPDVPSVLLELGFMSNKEDEKQLLDPEWRKKVSALLAAAVQSYRNTAVANGG
EEEECCCHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKRSANQLYSMRMIARHCGLRRLVAAVLFLLLPVVGVHAGEPVPLLAYAARIAGDDARTR
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEE
LVIEFDREPVFSVHYVANPVRIIVDLPETSFGLKPESLEPRGLFDAIRYGGMGAGASRLV
EEEEECCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHEE
LSAKEPTELTHAEVKPMEDGKGFRLVLDAERIGQDRFDKILAEQKWTGTVRAVKTDRPAA
EECCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCHHHHHHHHHCCCCCCEEEEEECCCCCC
APAKADAFVIAIDAGHGGIDTGAIGSVTKTEEKHVTLAFAKELVETLNREPGIEAFLTRE
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCC
RDEFLSLPQRVQIARQRGSSLFISLHADTLKQRDIRGATVYTISDKASDHLAADLAAREN
HHHHHCCHHHHHHHHHCCCEEEEEEEHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCC
LSDEIAGIPLESEPAEVADILIDLTRRETQAFSVNLARSVIASFEGQIKLINNPHRHAGF
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCE
RVLQAPDVPSVLLELGFMSNKEDEKQLLDPEWRKKVSALLAAAVQSYRNTAVANGG
EEEECCCHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]