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The map label for this gene is proS [H]

Identifier: 150396141

GI number: 150396141

Start: 990004

End: 991332

Strand: Direct

Name: proS [H]

Synonym: Smed_0918

Alternate gene names: 150396141

Gene position: 990004-991332 (Clockwise)

Preceding gene: 150396131

Following gene: 150396142

Centisome position: 26.18

GC content: 59.74

Gene sequence:

>1329_bases
ATGCGCCTGTCCCGCTTTTTCATGCCCATCCTGAAGGAAAATCCGAAGGAAGCGGAGATCGTTTCCCATCGATTGATGTT
GAGGACAGGCATGATCCGCCAGCAGTCCGCCGGCATCTACACGTGGCTGCCGCTCGGCAAGCGCGTGCTGGACAAGGTCA
ATGCGGTCATTCGCGAGGAGCAGAACCGGTCGGGCGCCATCGAGCTGCTGATGCCGACGCTGCAGTCGGCGGAGCTCTGG
CAGGAGAGCGGGCGATATGACGCCTATGGCAAGGAGATGCTGCGTATCAAGGACCGGCAGGACCGGCCCATGCTCTACGG
ACCGACGAATGAGGAGATGATCACCGACATCTTCCGCTCCTATGTCAAGTCCTACCGGAACCTGCCGCTGAACCTCTATC
ATATCCAGCTGAAGTTCCGCGACGAGATCCGCCCGCGCTTCGGAACCATGCGCTCGCGCGAGTTTCTGATGAAAGATGCC
TATTCCTTCGACCTGGACCGGGCAGGGGCGGAGCACGCATACAAGCGGATGTTCGCCGCCTATCTGCGCACTTTCGACCG
GCTGGGCCTGCGCGCGATCCCGATGCGCGCGGACACCGGTCCGATCGGCGGAAATCTGAGCCACGAGTTCATCATCCTTG
CAGACACGGGCGAGTCCGAGGTGTTCTGCCACAAGGACTTTCTCGGCTTCGACATTCCGGGCGAGGATACGAACTTCGAC
GATGTCGCGAGCATGAATGCGATCTTCGAAAAATGGACGTCGCGTTATGCGGCGACATCGGAAATGCATGAAGAGGCTGC
CTTCGACGCCATTCCGGAAGGTGAGCGCCTTTCCGCTCGCGGGATCGAGGTGGGGCACATCTTCTATTTCGGTACCAAAT
ATTCGGAGGCGATGGGAGCCAAGGTGCTTGGACCGGATGGCAAGGAACACACGGTGCACATGGGCTCCTATGGGATCGGC
CCGACCCGCCTCGTGCCTGCCATCATCGAAGCGTCGCATGACGATAACGGCATTATCTGGCCGAAGGGCATCGCCCCCTT
CGACATTGTCGTCATCAACATGAAAACGGGCGATGATGCTTGCGATACGGCTTGCGGCAGGCTCTACTCCGATCTGGGCA
AGGCTGGATTCGACGTTCTTCTTGACGACACCGACGAGCGTGCCGGCGGAAAGTTTGCGACCGCCGACATCATCGGTGTG
CCGGTGCAGGTGATCGTCGGGCCTAGGTCGATCGCAAATGGCGAGGTCGAAGTGAAGGACCGCAAGACCGGTGCGCGAGA
GACGATGACAGTCGAAGCGGCGATCAACAAACTGGTCGCGGCGAGATAA

Upstream 100 bases:

>100_bases
AGGCAGCAGCGGCCTTCATTACGTTGTAGAGGCGGCCGAAGCCAACGTCATTTCGCCCGAATCCGTCGATAGTGCGAATT
CACCAGTTCTGGAACCCGTC

Downstream 100 bases:

>100_bases
CAGGCGAAGGGATCAATGATGACTGCGGCGACGGACGAGCAAGTGCAGGCTGCCGCTCCATCCAGCATACCTTCCGGCCG
TCCCTTCTCCGCTTTCGAGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MRLSRFFMPILKENPKEAEIVSHRLMLRTGMIRQQSAGIYTWLPLGKRVLDKVNAVIREEQNRSGAIELLMPTLQSAELW
QESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMITDIFRSYVKSYRNLPLNLYHIQLKFRDEIRPRFGTMRSREFLMKDA
YSFDLDRAGAEHAYKRMFAAYLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFLGFDIPGEDTNFD
DVASMNAIFEKWTSRYAATSEMHEEAAFDAIPEGERLSARGIEVGHIFYFGTKYSEAMGAKVLGPDGKEHTVHMGSYGIG
PTRLVPAIIEASHDDNGIIWPKGIAPFDIVVINMKTGDDACDTACGRLYSDLGKAGFDVLLDDTDERAGGKFATADIIGV
PVQVIVGPRSIANGEVEVKDRKTGARETMTVEAAINKLVAAR

Sequences:

>Translated_442_residues
MRLSRFFMPILKENPKEAEIVSHRLMLRTGMIRQQSAGIYTWLPLGKRVLDKVNAVIREEQNRSGAIELLMPTLQSAELW
QESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMITDIFRSYVKSYRNLPLNLYHIQLKFRDEIRPRFGTMRSREFLMKDA
YSFDLDRAGAEHAYKRMFAAYLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFLGFDIPGEDTNFD
DVASMNAIFEKWTSRYAATSEMHEEAAFDAIPEGERLSARGIEVGHIFYFGTKYSEAMGAKVLGPDGKEHTVHMGSYGIG
PTRLVPAIIEASHDDNGIIWPKGIAPFDIVVINMKTGDDACDTACGRLYSDLGKAGFDVLLDDTDERAGGKFATADIIGV
PVQVIVGPRSIANGEVEVKDRKTGARETMTVEAAINKLVAAR
>Mature_442_residues
MRLSRFFMPILKENPKEAEIVSHRLMLRTGMIRQQSAGIYTWLPLGKRVLDKVNAVIREEQNRSGAIELLMPTLQSAELW
QESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMITDIFRSYVKSYRNLPLNLYHIQLKFRDEIRPRFGTMRSREFLMKDA
YSFDLDRAGAEHAYKRMFAAYLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFLGFDIPGEDTNFD
DVASMNAIFEKWTSRYAATSEMHEEAAFDAIPEGERLSARGIEVGHIFYFGTKYSEAMGAKVLGPDGKEHTVHMGSYGIG
PTRLVPAIIEASHDDNGIIWPKGIAPFDIVVINMKTGDDACDTACGRLYSDLGKAGFDVLLDDTDERAGGKFATADIIGV
PVQVIVGPRSIANGEVEVKDRKTGARETMTVEAAINKLVAAR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=442, Percent_Identity=35.5203619909502, Blast_Score=252, Evalue=5e-67,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=57.0135746606335, Blast_Score=279, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI115532348, Length=430, Percent_Identity=33.953488372093, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=35.9550561797753, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=316, Percent_Identity=30.6962025316456, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24656200, Length=424, Percent_Identity=32.0754716981132, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 49641; Mature: 49641

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRFFMPILKENPKEAEIVSHRLMLRTGMIRQQSAGIYTWLPLGKRVLDKVNAVIREE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCEEEEHHCCHHHHHHHHHHHHHH
QNRSGAIELLMPTLQSAELWQESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMITDIFRS
CCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHH
YVKSYRNLPLNLYHIQLKFRDEIRPRFGTMRSREFLMKDAYSFDLDRAGAEHAYKRMFAA
HHHHHHCCCEEEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHH
YLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFLGFDIPGEDTNFD
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCHH
DVASMNAIFEKWTSRYAATSEMHEEAAFDAIPEGERLSARGIEVGHIFYFGTKYSEAMGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHHHCCC
KVLGPDGKEHTVHMGSYGIGPTRLVPAIIEASHDDNGIIWPKGIAPFDIVVINMKTGDDA
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEECCCCHH
CDTACGRLYSDLGKAGFDVLLDDTDERAGGKFATADIIGVPVQVIVGPRSIANGEVEVKD
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCEEEEECCCCCCCCEEEEEE
RKTGARETMTVEAAINKLVAAR
CCCCCCHHEEHHHHHHHHHCCC
>Mature Secondary Structure
MRLSRFFMPILKENPKEAEIVSHRLMLRTGMIRQQSAGIYTWLPLGKRVLDKVNAVIREE
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCEEEEHHCCHHHHHHHHHHHHHH
QNRSGAIELLMPTLQSAELWQESGRYDAYGKEMLRIKDRQDRPMLYGPTNEEMITDIFRS
CCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHH
YVKSYRNLPLNLYHIQLKFRDEIRPRFGTMRSREFLMKDAYSFDLDRAGAEHAYKRMFAA
HHHHHHCCCEEEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHH
YLRTFDRLGLRAIPMRADTGPIGGNLSHEFIILADTGESEVFCHKDFLGFDIPGEDTNFD
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCHH
DVASMNAIFEKWTSRYAATSEMHEEAAFDAIPEGERLSARGIEVGHIFYFGTKYSEAMGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHHHCCC
KVLGPDGKEHTVHMGSYGIGPTRLVPAIIEASHDDNGIIWPKGIAPFDIVVINMKTGDDA
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEECCCCHH
CDTACGRLYSDLGKAGFDVLLDDTDERAGGKFATADIIGVPVQVIVGPRSIANGEVEVKD
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCEEEEECCCCCCCCEEEEEE
RKTGARETMTVEAAINKLVAAR
CCCCCCHHEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]