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The map label for this gene is thcD [H]

Identifier: 150396131

GI number: 150396131

Start: 980177

End: 981469

Strand: Direct

Name: thcD [H]

Synonym: Smed_0908

Alternate gene names: 150396131

Gene position: 980177-981469 (Clockwise)

Preceding gene: 150396130

Following gene: 150396141

Centisome position: 25.92

GC content: 62.34

Gene sequence:

>1293_bases
ATGCCTGAGAGAATAGTCATCATCGGCGGCGGTCAGGCGGGAGGGCGCGTCGCACAGATTCTGGCAAATTCGTCGGCGGA
TTTTCACATCTCCCTCATCGGCCGAGAGCCGCACCCCCCGTACAACCGCCCCCCTCTGTCCAAGGGCGTACTCCTGGCGA
AGTCCGGCTTCGAGGATTGCGCGATCTGGCGAGAGGGCGATGGAGCGGCTGGTAAAATCCGGTTTCATGCTGGCGTAGCT
GCGAAGATGATCGATACAGGCGCGAAGAACGTCGGCTTGGATGACGGCCGCGTAATCGAATATGACAGGCTGGTCCTTGC
CACCGGATCGCGCGTCCGGCGGGTGTCGATGCCGGGCGCCGATTGTTCCGGCGTGTACATGCTGCGGACTTTCGACGATG
CGGTTAAAATCGCGAAACAGTTTCACAGCGGCCTACGTCTCGTGGTGGTTGGAGGCGGCTTCATAGGGCTTGAGATCGCA
GCCGCCGCGCGTACGCGCGGACTGCACACGGTCGTCGTCGAAGCGACCAATCGGCTGCTGTCCCGCATCGCGCCGCAAAG
CATCGGCGACGCCCTTGCGCGGCATCACGAACAGGCGGGCGTTTCGTTTCGCGTCGGATGCATGGTCGAGCGGTTGATCT
CCACCCGTTCCGGCAAATTGAAGTCGGCTCTTTTGTCCAATGGAGAAACGATCCCCTGCGACCTGGCCATTGTCGGTGTG
GGGGTCACCGCCGATACCGAATTGGCAGCAAGTGCGGGTCTCGACGTACAGGTCGGTATAAGAACCGATGCGACGCTTCG
AACTTCAGATCCGAATATCTTCGCCTGTGGCGATGCGGTTTCCTTCTGGCACCCCTTGTTCGAGCGGCATGTCCGGGTGG
AGGCATGGCAAAATGCCGAGGATCACGCACGTGTGGTGGCGGGACGGCTGATGGGCCAGGAGACGATCTGTGACACCGTC
CCCTTCTTCTGGTCGGACCAGTACGAACTATCGATGCAGATCGTTGGCCTGCCGCAGTACGGCAGCTTTGTCGTGTCGCA
CGCGACCGAGGATGCGCAGATCCTGTATCATCTGGACCCGCGCGGCCGTCTCGTCGGTGCGACCGGCCTTGGGCCGCACC
AACTGATCGGAAGGAAGATCAGAGCAGCGCGTCAAGCAATCGCCGGGCGCAGCTGCCCGGATGCGCAGGCGCTCAAGGGT
GGCCTGATCCGGTCGGATGCGCCGCCCGAAAGCGGCAACCTCGAGCGGGAGGAGGAAAACCCTGCGCCGTTTTCCGCCCG
CATCCCGCTCTAA

Upstream 100 bases:

>100_bases
GAAGGATGAGTATCGGTCATCTTGACAAGGCCGCTTCAAGCAATGTGGAATATCCGCGTATATTCAAGAATTTGCCGTAA
CAAGCCAGAGTCGTGGGCCA

Downstream 100 bases:

>100_bases
TCGTCCGCAAGCCGGATATAGACCAAGCCATCTTCGACTTTTGCCGGGAACGTCCTGAGGTTGACGCAAACCGGCGGGTT
CAGCGCCTCGCCGGTGCGGA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPERIVIIGGGQAGGRVAQILANSSADFHISLIGREPHPPYNRPPLSKGVLLAKSGFEDCAIWREGDGAAGKIRFHAGVA
AKMIDTGAKNVGLDDGRVIEYDRLVLATGSRVRRVSMPGADCSGVYMLRTFDDAVKIAKQFHSGLRLVVVGGGFIGLEIA
AAARTRGLHTVVVEATNRLLSRIAPQSIGDALARHHEQAGVSFRVGCMVERLISTRSGKLKSALLSNGETIPCDLAIVGV
GVTADTELAASAGLDVQVGIRTDATLRTSDPNIFACGDAVSFWHPLFERHVRVEAWQNAEDHARVVAGRLMGQETICDTV
PFFWSDQYELSMQIVGLPQYGSFVVSHATEDAQILYHLDPRGRLVGATGLGPHQLIGRKIRAARQAIAGRSCPDAQALKG
GLIRSDAPPESGNLEREEENPAPFSARIPL

Sequences:

>Translated_430_residues
MPERIVIIGGGQAGGRVAQILANSSADFHISLIGREPHPPYNRPPLSKGVLLAKSGFEDCAIWREGDGAAGKIRFHAGVA
AKMIDTGAKNVGLDDGRVIEYDRLVLATGSRVRRVSMPGADCSGVYMLRTFDDAVKIAKQFHSGLRLVVVGGGFIGLEIA
AAARTRGLHTVVVEATNRLLSRIAPQSIGDALARHHEQAGVSFRVGCMVERLISTRSGKLKSALLSNGETIPCDLAIVGV
GVTADTELAASAGLDVQVGIRTDATLRTSDPNIFACGDAVSFWHPLFERHVRVEAWQNAEDHARVVAGRLMGQETICDTV
PFFWSDQYELSMQIVGLPQYGSFVVSHATEDAQILYHLDPRGRLVGATGLGPHQLIGRKIRAARQAIAGRSCPDAQALKG
GLIRSDAPPESGNLEREEENPAPFSARIPL
>Mature_429_residues
PERIVIIGGGQAGGRVAQILANSSADFHISLIGREPHPPYNRPPLSKGVLLAKSGFEDCAIWREGDGAAGKIRFHAGVAA
KMIDTGAKNVGLDDGRVIEYDRLVLATGSRVRRVSMPGADCSGVYMLRTFDDAVKIAKQFHSGLRLVVVGGGFIGLEIAA
AARTRGLHTVVVEATNRLLSRIAPQSIGDALARHHEQAGVSFRVGCMVERLISTRSGKLKSALLSNGETIPCDLAIVGVG
VTADTELAASAGLDVQVGIRTDATLRTSDPNIFACGDAVSFWHPLFERHVRVEAWQNAEDHARVVAGRLMGQETICDTVP
FFWSDQYELSMQIVGLPQYGSFVVSHATEDAQILYHLDPRGRLVGATGLGPHQLIGRKIRAARQAIAGRSCPDAQALKGG
LIRSDAPPESGNLEREEENPAPFSARIPL

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=337, Percent_Identity=30.5637982195846, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI226437568, Length=337, Percent_Identity=30.5637982195846, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI21389617, Length=337, Percent_Identity=30.5637982195846, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI22202629, Length=369, Percent_Identity=26.0162601626016, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI4757732, Length=369, Percent_Identity=26.0162601626016, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1788892, Length=403, Percent_Identity=31.5136476426799, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789765, Length=288, Percent_Identity=29.8611111111111, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789065, Length=280, Percent_Identity=27.1428571428571, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=330, Percent_Identity=29.0909090909091, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24639257, Length=359, Percent_Identity=26.7409470752089, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI281359715, Length=359, Percent_Identity=26.7409470752089, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI281359713, Length=359, Percent_Identity=26.7409470752089, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24639250, Length=359, Percent_Identity=26.7409470752089, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI18543267, Length=359, Percent_Identity=26.7409470752089, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24639252, Length=359, Percent_Identity=26.7409470752089, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24585130, Length=378, Percent_Identity=22.4867724867725, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI28573993, Length=364, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581020, Length=364, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 45980; Mature: 45849

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERIVIIGGGQAGGRVAQILANSSADFHISLIGREPHPPYNRPPLSKGVLLAKSGFEDC
CCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCC
AIWREGDGAAGKIRFHAGVAAKMIDTGAKNVGLDDGRVIEYDRLVLATGSRVRRVSMPGA
EEEECCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCEEEECEEEEECCCCEEEEECCCC
DCSGVYMLRTFDDAVKIAKQFHSGLRLVVVGGGFIGLEIAAAARTRGLHTVVVEATNRLL
CCCCEEEEEEHHHHHHHHHHHHCCCEEEEEECCEEEEEEEEHHHHCCCEEEEHHHHHHHH
SRIAPQSIGDALARHHEQAGVSFRVGCMVERLISTRSGKLKSALLSNGETIPCDLAIVGV
HHHCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEE
GVTADTELAASAGLDVQVGIRTDATLRTSDPNIFACGDAVSFWHPLFERHVRVEAWQNAE
CCCCCCHHHHHCCCEEEEEECCCCEEEECCCCEEEECCHHHHHHHHHHHCEEEEECCCCH
DHARVVAGRLMGQETICDTVPFFWSDQYELSMQIVGLPQYGSFVVSHATEDAQILYHLDP
HHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCEEEEEEECC
RGRLVGATGLGPHQLIGRKIRAARQAIAGRSCPDAQALKGGLIRSDAPPESGNLEREEEN
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCC
PAPFSARIPL
CCCCEEECCC
>Mature Secondary Structure 
PERIVIIGGGQAGGRVAQILANSSADFHISLIGREPHPPYNRPPLSKGVLLAKSGFEDC
CCEEEEEECCCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCC
AIWREGDGAAGKIRFHAGVAAKMIDTGAKNVGLDDGRVIEYDRLVLATGSRVRRVSMPGA
EEEECCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCEEEECEEEEECCCCEEEEECCCC
DCSGVYMLRTFDDAVKIAKQFHSGLRLVVVGGGFIGLEIAAAARTRGLHTVVVEATNRLL
CCCCEEEEEEHHHHHHHHHHHHCCCEEEEEECCEEEEEEEEHHHHCCCEEEEHHHHHHHH
SRIAPQSIGDALARHHEQAGVSFRVGCMVERLISTRSGKLKSALLSNGETIPCDLAIVGV
HHHCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEE
GVTADTELAASAGLDVQVGIRTDATLRTSDPNIFACGDAVSFWHPLFERHVRVEAWQNAE
CCCCCCHHHHHCCCEEEEEECCCCEEEECCCCEEEECCHHHHHHHHHHHCEEEEECCCCH
DHARVVAGRLMGQETICDTVPFFWSDQYELSMQIVGLPQYGSFVVSHATEDAQILYHLDP
HHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCEEEEEEECC
RGRLVGATGLGPHQLIGRKIRAARQAIAGRSCPDAQALKGGLIRSDAPPESGNLEREEEN
CCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCC
PAPFSARIPL
CCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]