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The map label for this gene is hupB

Identifier: 150396098

GI number: 150396098

Start: 936193

End: 936465

Strand: Direct

Name: hupB

Synonym: Smed_0875

Alternate gene names: 150396098

Gene position: 936193-936465 (Clockwise)

Preceding gene: 150396097

Following gene: 150396099

Centisome position: 24.75

GC content: 60.07

Gene sequence:

>273_bases
ATGAACAAAAATGAGCTCGTGGCTGCCGTAGCCGACAAGGCCGGACTCTCGAAGGCAGATGCGTCTTCTGCCGTTGATGC
AGTTTTTGAGACCATTCAGGGCGAACTGAAGAACGGCGGCGACATCCGCCTCGTTGGCTTCGGCAATTTCTCCGTATCGC
GCCGCGAGGCTAGCAAGGGCCGCAACCCGTCGACGGGTGCGGAAGTTGACATTCCGGCACGCAACGTGCCGAAGTTCACG
GCCGGCAAGGGCCTCAAGGACGCCGTCAACTGA

Upstream 100 bases:

>100_bases
GGGATTAGGCGCAATTGGGAATGGCAAGACGTGGGCAATGCTCAAGCTGCCCCGCCTTAGAAAGACAGTCGTTTCGAACC
AGCATGAAAGGGGTGGAAAC

Downstream 100 bases:

>100_bases
TAGAACGATTTAAGCGGCGGCAACTGCCGTCTGCGATTAGTTCATGTTCCGTCTGTCGCGGCCTTCGCCATCGGCGGCGC
TCGTAGGCAGGTAGATTGAG

Product: histone family protein DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKNELVAAVADKAGLSKADASSAVDAVFETIQGELKNGGDIRLVGFGNFSVSRREASKGRNPSTGAEVDIPARNVPKFT
AGKGLKDAVN

Sequences:

>Translated_90_residues
MNKNELVAAVADKAGLSKADASSAVDAVFETIQGELKNGGDIRLVGFGNFSVSRREASKGRNPSTGAEVDIPARNVPKFT
AGKGLKDAVN
>Mature_90_residues
MNKNELVAAVADKAGLSKADASSAVDAVFETIQGELKNGGDIRLVGFGNFSVSRREASKGRNPSTGAEVDIPARNVPKFT
AGKGLKDAVN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=52.2222222222222, Blast_Score=92, Evalue=5e-21,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=49.438202247191, Blast_Score=91, Evalue=1e-20,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=42.6966292134831, Blast_Score=74, Evalue=1e-15,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): DBH_RHIME (P02344)

Other databases:

- EMBL:   AF167159
- EMBL:   AL591688
- PIR:   S00053
- RefSeq:   NP_385364.1
- ProteinModelPortal:   P02344
- SMR:   P02344
- GeneID:   1232903
- GenomeReviews:   AL591688_GR
- KEGG:   sme:SMc01906
- NMPDR:   fig|266834.1.peg.2552
- HOGENOM:   HBG705085
- OMA:   NESKTHF
- ProtClustDB:   CLSK785191
- BioCyc:   SMEL266834:SMC01906-MONOMER
- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992
- Gene3D:   G3DSA:4.10.520.10
- PRINTS:   PR01727
- SMART:   SM00411

Pfam domain/function: PF00216 Bac_DNA_binding; SSF47729 IHF_like_DNA_bnd

EC number: NA

Molecular weight: Translated: 9303; Mature: 9303

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNELVAAVADKAGLSKADASSAVDAVFETIQGELKNGGDIRLVGFGNFSVSRREASKG
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHCC
RNPSTGAEVDIPARNVPKFTAGKGLKDAVN
CCCCCCCEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKNELVAAVADKAGLSKADASSAVDAVFETIQGELKNGGDIRLVGFGNFSVSRREASKG
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHCC
RNPSTGAEVDIPARNVPKFTAGKGLKDAVN
CCCCCCCEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6299736; 10762258; 11481430; 7049166