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The map label for this gene is nusB [H]

Identifier: 150396046

GI number: 150396046

Start: 884528

End: 885010

Strand: Direct

Name: nusB [H]

Synonym: Smed_0822

Alternate gene names: 150396046

Gene position: 884528-885010 (Clockwise)

Preceding gene: 150396045

Following gene: 150396047

Centisome position: 23.39

GC content: 61.08

Gene sequence:

>483_bases
GTGACGAACACCCCTTCGGATCAGCCACTGAAACAGGTCAATCAGCGTGGGGCCGCCCGCCTCGCAGCCGTTCAGGCACT
TTATCAGATGGATGTCGGCGGGACCGGCGTCCTGGAGATCGTCGCCGAATATGAGGAACATCGTCTCGGCAAGGAACTGG
ATGGCGACACCTATCTCAGGGCGGACGCCTCCTGGTTCCGATCGATCGTTGCTGGCGTTGTGCGCGACCAGCGCAAGCTC
GATCCGTTGATCGGCTCGGCGCTTCAGGACGATTGGGCGCTTTCCCGTCTCGACTCGACCGTGCGTGCGATCCTGCGCGC
CGGTACCTTCGAGATTCTCGAACGAAAAGACGTGCCGGTCCCGGTGATCGTGACCGAATATGTCGAGATCGCCAAGGCCT
TCTTCCAGGATGAGGAGCCGAAGCTCGTCAACGCCGTCCTCGACCGGATCGCCAAGCAGGTTCGCGGCGAACAGAGAAAG
TAA

Upstream 100 bases:

>100_bases
CCTGGGCAAGGGCCCGCCGGTCGGAGGGCGACAAGGGTGGGTTCGCCGCCCGCGCGGCCCTTACCATGATCGAACTGAAG
CAAAGATTGGGCGCAGACAA

Downstream 100 bases:

>100_bases
ACGCGGATGGTCGCCGAGACGGCAGGCACGGGGACGGTTCTGGGCGAGGCGCGCCGCAACGTCCAGTTGCTCACGGTGGC
TCAGGCGCTGCTCGGGGTTG

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTNTPSDQPLKQVNQRGAARLAAVQALYQMDVGGTGVLEIVAEYEEHRLGKELDGDTYLRADASWFRSIVAGVVRDQRKL
DPLIGSALQDDWALSRLDSTVRAILRAGTFEILERKDVPVPVIVTEYVEIAKAFFQDEEPKLVNAVLDRIAKQVRGEQRK

Sequences:

>Translated_160_residues
MTNTPSDQPLKQVNQRGAARLAAVQALYQMDVGGTGVLEIVAEYEEHRLGKELDGDTYLRADASWFRSIVAGVVRDQRKL
DPLIGSALQDDWALSRLDSTVRAILRAGTFEILERKDVPVPVIVTEYVEIAKAFFQDEEPKLVNAVLDRIAKQVRGEQRK
>Mature_159_residues
TNTPSDQPLKQVNQRGAARLAAVQALYQMDVGGTGVLEIVAEYEEHRLGKELDGDTYLRADASWFRSIVAGVVRDQRKLD
PLIGSALQDDWALSRLDSTVRAILRAGTFEILERKDVPVPVIVTEYVEIAKAFFQDEEPKLVNAVLDRIAKQVRGEQRK

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=138, Percent_Identity=31.1594202898551, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 17906; Mature: 17774

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTPSDQPLKQVNQRGAARLAAVQALYQMDVGGTGVLEIVAEYEEHRLGKELDGDTYLR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEH
ADASWFRSIVAGVVRDQRKLDPLIGSALQDDWALSRLDSTVRAILRAGTFEILERKDVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVIVTEYVEIAKAFFQDEEPKLVNAVLDRIAKQVRGEQRK
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNTPSDQPLKQVNQRGAARLAAVQALYQMDVGGTGVLEIVAEYEEHRLGKELDGDTYLR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEH
ADASWFRSIVAGVVRDQRKLDPLIGSALQDDWALSRLDSTVRAILRAGTFEILERKDVPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PVIVTEYVEIAKAFFQDEEPKLVNAVLDRIAKQVRGEQRK
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]