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The map label for this gene is ribH [H]

Identifier: 150396045

GI number: 150396045

Start: 884070

End: 884531

Strand: Direct

Name: ribH [H]

Synonym: Smed_0821

Alternate gene names: 150396045

Gene position: 884070-884531 (Clockwise)

Preceding gene: 150396043

Following gene: 150396046

Centisome position: 23.38

GC content: 62.12

Gene sequence:

>462_bases
ATGGCGAAGATCAAGCCCGTCCACATCCTGATTGTGGAAGCGCGTTTCTATGACGATATGGCCGACGCGATGCTCGACGG
AGCGAAACATGCACTCGATGCAGCCGGCGCCACTTATGATATTGTGACGGTGCCGGGGGCGCTGGAAATTCCCGCGGCGA
TCGCCATGGCGCTCGACGGCGTAGACGAAGGCGGGACGGAATATGACGGCTTCGTTGCACTCGGCATGGTGATCCGCGGC
GAAACCTACCATTTCGACATCGTCGCCAACGAGTCCGCACGCGCGCTGATGGATTTGGCGGTCAGCGAGAGCCTGGCGCT
GGGCAATGGCATCCTGACGGTCGAGAACGACGATCAGGCCTGGGCAAGGGCCCGCCGGTCGGAGGGCGACAAGGGTGGGT
TCGCCGCCCGCGCGGCCCTTACCATGATCGAACTGAAGCAAAGATTGGGCGCAGACAAGTGA

Upstream 100 bases:

>100_bases
GTTTCGTTAACCGAATTTACTTGCCATTCCGTGCCGGGCATGGTTTGACCGCCGCCTGCACCGGCCAAGTCCGGGCCGAA
TTTTTCAAGACAGGTGATCC

Downstream 100 bases:

>100_bases
CGAACACCCCTTCGGATCAGCCACTGAAACAGGTCAATCAGCGTGGGGCCGCCCGCCTCGCAGCCGTTCAGGCACTTTAT
CAGATGGATGTCGGCGGGAC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase 1; Lumazine synthase 1; Riboflavin synthase 1 beta chain [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MAKIKPVHILIVEARFYDDMADAMLDGAKHALDAAGATYDIVTVPGALEIPAAIAMALDGVDEGGTEYDGFVALGMVIRG
ETYHFDIVANESARALMDLAVSESLALGNGILTVENDDQAWARARRSEGDKGGFAARAALTMIELKQRLGADK

Sequences:

>Translated_153_residues
MAKIKPVHILIVEARFYDDMADAMLDGAKHALDAAGATYDIVTVPGALEIPAAIAMALDGVDEGGTEYDGFVALGMVIRG
ETYHFDIVANESARALMDLAVSESLALGNGILTVENDDQAWARARRSEGDKGGFAARAALTMIELKQRLGADK
>Mature_152_residues
AKIKPVHILIVEARFYDDMADAMLDGAKHALDAAGATYDIVTVPGALEIPAAIAMALDGVDEGGTEYDGFVALGMVIRGE
TYHFDIVANESARALMDLAVSESLALGNGILTVENDDQAWARARRSEGDKGGFAARAALTMIELKQRLGADK

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=149, Percent_Identity=38.9261744966443, Blast_Score=81, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324429, Length=121, Percent_Identity=29.7520661157025, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 16210; Mature: 16078

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKPVHILIVEARFYDDMADAMLDGAKHALDAAGATYDIVTVPGALEIPAAIAMALDG
CCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCC
VDEGGTEYDGFVALGMVIRGETYHFDIVANESARALMDLAVSESLALGNGILTVENDDQA
CCCCCCCCCCEEEEEEEEECCEEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHH
WARARRSEGDKGGFAARAALTMIELKQRLGADK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKIKPVHILIVEARFYDDMADAMLDGAKHALDAAGATYDIVTVPGALEIPAAIAMALDG
CCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCC
VDEGGTEYDGFVALGMVIRGETYHFDIVANESARALMDLAVSESLALGNGILTVENDDQA
CCCCCCCCCCEEEEEEEEECCEEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHH
WARARRSEGDKGGFAARAALTMIELKQRLGADK
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]