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The map label for this gene is dapA [H]

Identifier: 150395897

GI number: 150395897

Start: 725763

End: 726647

Strand: Direct

Name: dapA [H]

Synonym: Smed_0673

Alternate gene names: 150395897

Gene position: 725763-726647 (Clockwise)

Preceding gene: 150395892

Following gene: 150395898

Centisome position: 19.19

GC content: 60.23

Gene sequence:

>885_bases
ATGTTCAAGGGTTCCATTCCCGCACTCGTAACGCCGTTCACCGCTGCAGGCTCGGTCGATGCGGATAGTTTCGTCGCGCA
TGTGGAGTGGCAGATAAAAGAGGGGAGCCACGGACTTGTGCCCGTGGGCACGACGGGTGAATCGCCGACACTCTCCCATG
ACGAGCACAAGAAAGTAGTCGAACTTTGTGTCGAGGCATCGGCGCGGCGCGTGCCGGTCATCGCCGGTGCAGGCTCCAAC
AATACGATCGAGGCGGTCGACTTTGCGCAGCACGCGGAAAAGGCCGGCGCGGATGCCATCCTCGTCGTCACGCCCTATTA
CAACAAGCCGACACAGAAGGGGCTCTTCGCCCACTTCGCCGCGATCGCAGAAAGTGTGAAGCTGCCGATCGTCATTTATA
ATATTCCGGGCCGTTCGGTCGTCGACATGAGCGTCGAGACCATGGCGGCGCTCGCAAAGGCACATCCGACGATTATCGGG
GTCAAGGATGCGACCGGTAAAATCGAGAGGGTCTCCGAGCAGCGGATGGCATGCGGCAAGGCTTTCGTCCAGCTTTCGGG
AGAGGACGCAACTGCGCTCGGCTTCAATGCGCATGGCGGCGTCGGTTGCATCTCCGTTACCGCCAATGTCGCGCCGCGAC
TGTGCGCCGAGTTTCAGGAGGCAACCCTTGCCGCCGACTTTGGCAAGGCATTGGAGTACCAGGACAAATTGATGCCCCTT
CACAAGGCGATCTTTCTGGAGCCGGGCGTCTGCGGCGCAAAATATGCGCTGAACCGCCTCGGAAGGATGAACCGTGATGT
TCGTTCGCCGCTGTTGTCGGCGCTTGAACCCGCGACGGAATCGGCAATCGACGCGGCGCTCAGGCATGCAGGACTGATGA
ACTGA

Upstream 100 bases:

>100_bases
CCGCAGCCACGGCACATGATTATGGTGCGGCAGTTTTTTAACCGTCGATTGCGGCCAAAGCGTGAGCGCATTTTGCGCTG
CTGGTCGTCAGGAGTTGTGC

Downstream 100 bases:

>100_bases
TGGCACCGAAAGGCAGCGAACGGACGGTCAAAAAGGTCGTCGCGGAAAACCGGAAGGCCCGCTTCAACTACGAGATCGTG
GATACCTACGAGGCCGGGCT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MFKGSIPALVTPFTAAGSVDADSFVAHVEWQIKEGSHGLVPVGTTGESPTLSHDEHKKVVELCVEASARRVPVIAGAGSN
NTIEAVDFAQHAEKAGADAILVVTPYYNKPTQKGLFAHFAAIAESVKLPIVIYNIPGRSVVDMSVETMAALAKAHPTIIG
VKDATGKIERVSEQRMACGKAFVQLSGEDATALGFNAHGGVGCISVTANVAPRLCAEFQEATLAADFGKALEYQDKLMPL
HKAIFLEPGVCGAKYALNRLGRMNRDVRSPLLSALEPATESAIDAALRHAGLMN

Sequences:

>Translated_294_residues
MFKGSIPALVTPFTAAGSVDADSFVAHVEWQIKEGSHGLVPVGTTGESPTLSHDEHKKVVELCVEASARRVPVIAGAGSN
NTIEAVDFAQHAEKAGADAILVVTPYYNKPTQKGLFAHFAAIAESVKLPIVIYNIPGRSVVDMSVETMAALAKAHPTIIG
VKDATGKIERVSEQRMACGKAFVQLSGEDATALGFNAHGGVGCISVTANVAPRLCAEFQEATLAADFGKALEYQDKLMPL
HKAIFLEPGVCGAKYALNRLGRMNRDVRSPLLSALEPATESAIDAALRHAGLMN
>Mature_294_residues
MFKGSIPALVTPFTAAGSVDADSFVAHVEWQIKEGSHGLVPVGTTGESPTLSHDEHKKVVELCVEASARRVPVIAGAGSN
NTIEAVDFAQHAEKAGADAILVVTPYYNKPTQKGLFAHFAAIAESVKLPIVIYNIPGRSVVDMSVETMAALAKAHPTIIG
VKDATGKIERVSEQRMACGKAFVQLSGEDATALGFNAHGGVGCISVTANVAPRLCAEFQEATLAADFGKALEYQDKLMPL
HKAIFLEPGVCGAKYALNRLGRMNRDVRSPLLSALEPATESAIDAALRHAGLMN

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family [H]

Homologues:

Organism=Homo sapiens, GI31543060, Length=296, Percent_Identity=27.7027027027027, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI13540533, Length=241, Percent_Identity=29.045643153527, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788823, Length=294, Percent_Identity=43.5374149659864, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI1786463, Length=266, Percent_Identity=33.0827067669173, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI87082415, Length=284, Percent_Identity=32.0422535211268, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1789620, Length=205, Percent_Identity=30.7317073170732, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.2.1.52 [H]

Molecular weight: Translated: 30970; Mature: 30970

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00665 DHDPS_1 ; PS00666 DHDPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGSIPALVTPFTAAGSVDADSFVAHVEWQIKEGSHGLVPVGTTGESPTLSHDEHKKVV
CCCCCCCCEECCCCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHHH
ELCVEASARRVPVIAGAGSNNTIEAVDFAQHAEKAGADAILVVTPYYNKPTQKGLFAHFA
HHHHHCCCCCCEEEEECCCCCCEEHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
AIAESVKLPIVIYNIPGRSVVDMSVETMAALAKAHPTIIGVKDATGKIERVSEQRMACGK
HHHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
AFVQLSGEDATALGFNAHGGVGCISVTANVAPRLCAEFQEATLAADFGKALEYQDKLMPL
HHEEECCCCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKAIFLEPGVCGAKYALNRLGRMNRDVRSPLLSALEPATESAIDAALRHAGLMN
HHHHEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFKGSIPALVTPFTAAGSVDADSFVAHVEWQIKEGSHGLVPVGTTGESPTLSHDEHKKVV
CCCCCCCCEECCCCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHHH
ELCVEASARRVPVIAGAGSNNTIEAVDFAQHAEKAGADAILVVTPYYNKPTQKGLFAHFA
HHHHHCCCCCCEEEEECCCCCCEEHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
AIAESVKLPIVIYNIPGRSVVDMSVETMAALAKAHPTIIGVKDATGKIERVSEQRMACGK
HHHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHH
AFVQLSGEDATALGFNAHGGVGCISVTANVAPRLCAEFQEATLAADFGKALEYQDKLMPL
HHEEECCCCCEEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HKAIFLEPGVCGAKYALNRLGRMNRDVRSPLLSALEPATESAIDAALRHAGLMN
HHHHEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]