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The map label for this gene is smpB [H]

Identifier: 150395898

GI number: 150395898

Start: 726647

End: 727126

Strand: Direct

Name: smpB [H]

Synonym: Smed_0674

Alternate gene names: 150395898

Gene position: 726647-727126 (Clockwise)

Preceding gene: 150395897

Following gene: 150395900

Centisome position: 19.21

GC content: 60.0

Gene sequence:

>480_bases
ATGGCACCGAAAGGCAGCGAACGGACGGTCAAAAAGGTCGTCGCGGAAAACCGGAAGGCCCGCTTCAACTACGAGATCGT
GGATACCTACGAGGCCGGGCTGGTGCTGACCGGCACGGAGGTCAAGTCGTTGCGCGAGGGAAAAGCCAATATTGCCGAAT
CATACGCGACCGACGAAGGCGGCGAGATCTGGCTCATCAACTCCTATCTCCCGGAATATCTGCAGGCGAACCGCTTCAAC
CACGAGACGCGCAGGCGTCGCAAGTTGCTCCTTTCGAGGAGGGAAGTGAACCGTCTGCAGGGCGCCGTGAACCGCGAAGG
CATGTCGCTGATACCGCTTCGCATCTATTTCAACGAGCGGGGGCGGGCCAAGCTGGAATTGGCGCTCGGCAAGGGCAAGA
AGCTCCACGACAAGCGCGAGACTTCCAAGGAACGCGACTGGAACCGACAGAAGAACCGGCTGCTCAAGGAACGCGGCTGA

Upstream 100 bases:

>100_bases
GGATGAACCGTGATGTTCGTTCGCCGCTGTTGTCGGCGCTTGAACCCGCGACGGAATCGGCAATCGACGCGGCGCTCAGG
CATGCAGGACTGATGAACTG

Downstream 100 bases:

>100_bases
GTTTCGTCGAGATCAGGCTGAGTTTCGTCGAGATCAGATTGGCTCTCATCCGCCTGCCGGCCCTTACTCCGCCGCGGGCG
GGCGAAGGGAAAAGCGGCAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAPKGSERTVKKVVAENRKARFNYEIVDTYEAGLVLTGTEVKSLREGKANIAESYATDEGGEIWLINSYLPEYLQANRFN
HETRRRRKLLLSRREVNRLQGAVNREGMSLIPLRIYFNERGRAKLELALGKGKKLHDKRETSKERDWNRQKNRLLKERG

Sequences:

>Translated_159_residues
MAPKGSERTVKKVVAENRKARFNYEIVDTYEAGLVLTGTEVKSLREGKANIAESYATDEGGEIWLINSYLPEYLQANRFN
HETRRRRKLLLSRREVNRLQGAVNREGMSLIPLRIYFNERGRAKLELALGKGKKLHDKRETSKERDWNRQKNRLLKERG
>Mature_158_residues
APKGSERTVKKVVAENRKARFNYEIVDTYEAGLVLTGTEVKSLREGKANIAESYATDEGGEIWLINSYLPEYLQANRFNH
ETRRRRKLLLSRREVNRLQGAVNREGMSLIPLRIYFNERGRAKLELALGKGKKLHDKRETSKERDWNRQKNRLLKERG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=157, Percent_Identity=40.7643312101911, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18559; Mature: 18427

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPKGSERTVKKVVAENRKARFNYEIVDTYEAGLVLTGTEVKSLREGKANIAESYATDEG
CCCCCHHHHHHHHHHCCCCCEECEEEEEECCCCEEEECHHHHHHHHCHHHHHHHHCCCCC
GEIWLINSYLPEYLQANRFNHETRRRRKLLLSRREVNRLQGAVNREGMSLIPLRIYFNER
CCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCC
GRAKLELALGKGKKLHDKRETSKERDWNRQKNRLLKERG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
APKGSERTVKKVVAENRKARFNYEIVDTYEAGLVLTGTEVKSLREGKANIAESYATDEG
CCCCHHHHHHHHHHCCCCCEECEEEEEECCCCEEEECHHHHHHHHCHHHHHHHHCCCCC
GEIWLINSYLPEYLQANRFNHETRRRRKLLLSRREVNRLQGAVNREGMSLIPLRIYFNER
CCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCC
GRAKLELALGKGKKLHDKRETSKERDWNRQKNRLLKERG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]