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The map label for this gene is proW [H]

Identifier: 150395875

GI number: 150395875

Start: 703967

End: 704854

Strand: Direct

Name: proW [H]

Synonym: Smed_0651

Alternate gene names: 150395875

Gene position: 703967-704854 (Clockwise)

Preceding gene: 150395874

Following gene: 150395877

Centisome position: 18.61

GC content: 56.76

Gene sequence:

>888_bases
ATGGAATGGCTGACCAAATTTCCGCACATGGACGACGATCGGCTTCGACAGCTGAAGAAGATAATCGATGAAGGGTTCCG
TAGCTTCACACGCGCCTATGGCGACGCGATCGAAGCCTTCTTCGATCCACTGCAATTCTTCCTGATCCATGCCGAACGTT
TCATGACGCGAACGCCCTGGCCGATCATTCTCATCCTGATCGCGCTGATCGCCTGGTTTGCCAGCCGCAACTGGAAGATA
GTCGCGGGCGCGATCGGGACCCTGCTCCTCATCGGCTATCTCGATATGTGGGACGATACGATGAAAACGATCTCGATGAT
CTTTGTCTGTACGGTTCTGTCGATAGCGGTGGGCATACCGATGGGCATCATCATGTCGCGCTCCGACCGTTTCCAGAATG
TCGTCAATCCGGTGCTCGACGTGATGCAGACCATGCCGAGCTTCGTCTACCTCATTCCCGTCGTCATGCTTCTCGGTATC
GGCAAGGTTCCAGGGCTCATCGCCGTAGTCATCTATGCAATCCCGCCGATGATCCGCCTCACCAACCTTGGAATCCGCCT
CGTCGACAAGGATGTGCTGGAGGCAGCTGACGCTTTCGGCTCGTCGAATTGGCAGAAGCTGAAGAATGTGCAGATGCCGC
TGGCGCTGCCGACAATCATGGCCGGGATCAATCAGACCATCATGATGGCACTCGCCATGGTCGTCATCGCCTCGATGATC
GGCGTTCAGGGCCTGGGGCAGCCGGTGTTGAAGGCGATCGCCAACCAGTATTTCACGCTCGGAATTTTCAACGGTCTCGC
CATCGTGGGAATCGCCATCATCTTCGACCGGGTTAGCCAGGCCTATGGGCGCCGACTCCAGCGACACCGCGAAATCATCC
ACGGGTAG

Upstream 100 bases:

>100_bases
CGCAGCTTGATCGTTTACACCGCGCGTTAGCGTTTGAAGGCAGGCGGCAAAGCAATAAAAACCATGAGCCGCCCAAAAGA
GTGCGAAGGGGAATCGACAC

Downstream 100 bases:

>100_bases
AAGCAAACGCTGCTTTTGAGTTGCCACTCCCCACAAGCTGGGGAGTGGTCTTACGGAATCGGGAGCAGGTACCCCCGACT
CCGCGAGCTGACCTCAGAAC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MEWLTKFPHMDDDRLRQLKKIIDEGFRSFTRAYGDAIEAFFDPLQFFLIHAERFMTRTPWPIILILIALIAWFASRNWKI
VAGAIGTLLLIGYLDMWDDTMKTISMIFVCTVLSIAVGIPMGIIMSRSDRFQNVVNPVLDVMQTMPSFVYLIPVVMLLGI
GKVPGLIAVVIYAIPPMIRLTNLGIRLVDKDVLEAADAFGSSNWQKLKNVQMPLALPTIMAGINQTIMMALAMVVIASMI
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGRRLQRHREIIHG

Sequences:

>Translated_295_residues
MEWLTKFPHMDDDRLRQLKKIIDEGFRSFTRAYGDAIEAFFDPLQFFLIHAERFMTRTPWPIILILIALIAWFASRNWKI
VAGAIGTLLLIGYLDMWDDTMKTISMIFVCTVLSIAVGIPMGIIMSRSDRFQNVVNPVLDVMQTMPSFVYLIPVVMLLGI
GKVPGLIAVVIYAIPPMIRLTNLGIRLVDKDVLEAADAFGSSNWQKLKNVQMPLALPTIMAGINQTIMMALAMVVIASMI
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGRRLQRHREIIHG
>Mature_295_residues
MEWLTKFPHMDDDRLRQLKKIIDEGFRSFTRAYGDAIEAFFDPLQFFLIHAERFMTRTPWPIILILIALIAWFASRNWKI
VAGAIGTLLLIGYLDMWDDTMKTISMIFVCTVLSIAVGIPMGIIMSRSDRFQNVVNPVLDVMQTMPSFVYLIPVVMLLGI
GKVPGLIAVVIYAIPPMIRLTNLGIRLVDKDVLEAADAFGSSNWQKLKNVQMPLALPTIMAGINQTIMMALAMVVIASMI
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGRRLQRHREIIHG

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=293, Percent_Identity=41.6382252559727, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1788451, Length=199, Percent_Identity=30.1507537688442, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788449, Length=189, Percent_Identity=28.042328042328, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786564, Length=158, Percent_Identity=25.9493670886076, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33024; Mature: 33024

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWLTKFPHMDDDRLRQLKKIIDEGFRSFTRAYGDAIEAFFDPLQFFLIHAERFMTRTPW
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
PIILILIALIAWFASRNWKIVAGAIGTLLLIGYLDMWDDTMKTISMIFVCTVLSIAVGIP
HHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MGIIMSRSDRFQNVVNPVLDVMQTMPSFVYLIPVVMLLGIGKVPGLIAVVIYAIPPMIRL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHH
TNLGIRLVDKDVLEAADAFGSSNWQKLKNVQMPLALPTIMAGINQTIMMALAMVVIASMI
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGRRLQRHREIIHG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]