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The map label for this gene is ispH [H]

Identifier: 150395751

GI number: 150395751

Start: 568944

End: 569954

Strand: Direct

Name: ispH [H]

Synonym: Smed_0527

Alternate gene names: 150395751

Gene position: 568944-569954 (Clockwise)

Preceding gene: 150395750

Following gene: 150395752

Centisome position: 15.04

GC content: 62.22

Gene sequence:

>1011_bases
ATGATGGCCTCATCCGTAGCAAAAACCCCGATCACCATCCGCCTCTGCGGGCCGCGTGGCTTCTGCGCGGGCGTCGACCG
GGCGATCCAGATCGTGGTGCTTGCGCTCAAGGAGTTCGGCGCGCCGGTCTATGTCCGTCATGAGATCGTGCACAATCGCT
ATGTCGTGGAAGGTCTTGAAGCCAAGGGTGCGATTTTCGTCGAGGAGCTCGACGAGATTCCACCCGAGCATCGCAAGCAG
CCCGTCGTCTTCTCCGCCCACGGCGTGCCGAAATCCGTCCCCGCGGATGCGGATGCGCGCAATCTCTTCTATCTCGATGC
CACATGCCCGCTCGTCTCGAAGGTGCACAAACAGGCTATGCGCCATCACCGTATGGGGCGCCATGTGGTGCTGATCGGCC
ATGCCGGCCATCCCGAGGTTATCGGCACCATGGGGCAACTGCCGGAGGGGACGGTCTCCCTCATCGAGACTGTCGAGGAT
GTCGACGTTTATACGCCCCCGGATCCGGACAATCTCGGCTTTGTTACGCAGACGACGCTCTCGGTGGATGATACCGCCGG
CGTCATCAAGCGGCTCCATGAGCGCTTTCCGAACCTGACTGCGCCTGCCGCCGACTCGATCTGCTACGCCACCACGAACC
GGCAGGAAGCGGTGAAGCAGGCTGCACCCGGCTGCGATCTTTTCCTCGTCGTCGGCGCCCCCAATTCTTCGAACTCGAAG
CGCCTGGTGGAAGTAGCGCTGAGGGCCGGGGCAAAGAAAGCCGTTCTGGTTCAGCGGGCTTCTGAAATTGACTGGGCGAC
GATCGGGGAAATCTCGACCGTCGGGTTGTCCGCCGGTGCCTCGGCGCCGGAGGTGATCGTCAATGAGATCATCGAAGCCT
TCCGCGAGCGCTACGACGCCGCGGTCGAGCTTGCCGACACGGTGGAGGAGAACGAGCACTTCCTCGTCAACCGCGAGCTC
AGGCATGTCGAACTGACCGGCGCCGACATGGCTTTCGTCAATGGTGAATAG

Upstream 100 bases:

>100_bases
CAGGCGGTAGGATGCGGCGGTTCTTCCTTGTGTGCCCGGTCGTTGCCGCTATATATGGCGCATACCGAAATCCGGCTTTG
TTCGTTCCCTTGGCGTTTTC

Downstream 100 bases:

>100_bases
AGCTGGGGTGAACAGAGCGGGGGTGAATAGAGCGAGCCGGCGTACTTCCCCTCGCACGCCGCTCCAAATTCCTCCAATTT
GCATCTCTGGAAAGTAGCGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MMASSVAKTPITIRLCGPRGFCAGVDRAIQIVVLALKEFGAPVYVRHEIVHNRYVVEGLEAKGAIFVEELDEIPPEHRKQ
PVVFSAHGVPKSVPADADARNLFYLDATCPLVSKVHKQAMRHHRMGRHVVLIGHAGHPEVIGTMGQLPEGTVSLIETVED
VDVYTPPDPDNLGFVTQTTLSVDDTAGVIKRLHERFPNLTAPAADSICYATTNRQEAVKQAAPGCDLFLVVGAPNSSNSK
RLVEVALRAGAKKAVLVQRASEIDWATIGEISTVGLSAGASAPEVIVNEIIEAFRERYDAAVELADTVEENEHFLVNREL
RHVELTGADMAFVNGE

Sequences:

>Translated_336_residues
MMASSVAKTPITIRLCGPRGFCAGVDRAIQIVVLALKEFGAPVYVRHEIVHNRYVVEGLEAKGAIFVEELDEIPPEHRKQ
PVVFSAHGVPKSVPADADARNLFYLDATCPLVSKVHKQAMRHHRMGRHVVLIGHAGHPEVIGTMGQLPEGTVSLIETVED
VDVYTPPDPDNLGFVTQTTLSVDDTAGVIKRLHERFPNLTAPAADSICYATTNRQEAVKQAAPGCDLFLVVGAPNSSNSK
RLVEVALRAGAKKAVLVQRASEIDWATIGEISTVGLSAGASAPEVIVNEIIEAFRERYDAAVELADTVEENEHFLVNREL
RHVELTGADMAFVNGE
>Mature_336_residues
MMASSVAKTPITIRLCGPRGFCAGVDRAIQIVVLALKEFGAPVYVRHEIVHNRYVVEGLEAKGAIFVEELDEIPPEHRKQ
PVVFSAHGVPKSVPADADARNLFYLDATCPLVSKVHKQAMRHHRMGRHVVLIGHAGHPEVIGTMGQLPEGTVSLIETVED
VDVYTPPDPDNLGFVTQTTLSVDDTAGVIKRLHERFPNLTAPAADSICYATTNRQEAVKQAAPGCDLFLVVGAPNSSNSK
RLVEVALRAGAKKAVLVQRASEIDWATIGEISTVGLSAGASAPEVIVNEIIEAFRERYDAAVELADTVEENEHFLVNREL
RHVELTGADMAFVNGE

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=313, Percent_Identity=50.4792332268371, Blast_Score=300, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 36388; Mature: 36388

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMASSVAKTPITIRLCGPRGFCAGVDRAIQIVVLALKEFGAPVYVRHEIVHNRYVVEGLE
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCHHEEECCC
AKGAIFVEELDEIPPEHRKQPVVFSAHGVPKSVPADADARNLFYLDATCPLVSKVHKQAM
CCCEEEHHHHHCCCHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
RHHRMGRHVVLIGHAGHPEVIGTMGQLPEGTVSLIETVEDVDVYTPPDPDNLGFVTQTTL
HHHHCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SVDDTAGVIKRLHERFPNLTAPAADSICYATTNRQEAVKQAAPGCDLFLVVGAPNSSNSK
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCH
RLVEVALRAGAKKAVLVQRASEIDWATIGEISTVGLSAGASAPEVIVNEIIEAFRERYDA
HHHHHHHHCCCCHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
AVELADTVEENEHFLVNRELRHVELTGADMAFVNGE
HHHHHHHHCCCCEEEEECCEEEEEECCCCEEEECCC
>Mature Secondary Structure
MMASSVAKTPITIRLCGPRGFCAGVDRAIQIVVLALKEFGAPVYVRHEIVHNRYVVEGLE
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCHHEEECCC
AKGAIFVEELDEIPPEHRKQPVVFSAHGVPKSVPADADARNLFYLDATCPLVSKVHKQAM
CCCEEEHHHHHCCCHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHH
RHHRMGRHVVLIGHAGHPEVIGTMGQLPEGTVSLIETVEDVDVYTPPDPDNLGFVTQTTL
HHHHCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SVDDTAGVIKRLHERFPNLTAPAADSICYATTNRQEAVKQAAPGCDLFLVVGAPNSSNSK
CCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCH
RLVEVALRAGAKKAVLVQRASEIDWATIGEISTVGLSAGASAPEVIVNEIIEAFRERYDA
HHHHHHHHCCCCHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
AVELADTVEENEHFLVNRELRHVELTGADMAFVNGE
HHHHHHHHCCCCEEEEECCEEEEEECCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]