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The map label for this gene is fmt

Identifier: 150395283

GI number: 150395283

Start: 57979

End: 58914

Strand: Direct

Name: fmt

Synonym: Smed_0055

Alternate gene names: 150395283

Gene position: 57979-58914 (Clockwise)

Preceding gene: 150395282

Following gene: 150395284

Centisome position: 1.53

GC content: 66.45

Gene sequence:

>936_bases
TTGCCGCTTCGCATCATCTTCATGGGTACACCGGAATTTTCCGTGCCGACGCTTCTTGCGCTCGCCGGGGCGGGTCACGA
GATTGCCGCCGTGTATACACAACCGCCGCGGCCCGGCGGGCGGCGCGGCCTCGATCTCCAGAAATCGCCGGTGCACCAGG
CTGCCGAGCGTCTCGACATTCCGGTCCTGACGCCCGTCAACTTCAAGGAAGCCGCGGACCGCCGAACCTTCCGGAATTTC
GGTGCCGATGCCGCGGTCGTGGTCGCCTACGGCCTGCTTCTGCCTGAGGAGATACTCTCGGGCACCCGCTGCGGCTGTTA
CAACGGTCATGCCTCGCTCCTGCCGCGCTGGCGGGGGGCGGCGCCGATCCAGCGGGCGATCATGGCCGGCGACCGGGAGA
CCGGCATGATGGTGATGAAAATGGACAAGGGGCTCGATACCGGTCCCGTCGCTCTCGCCCGGTCGGTGCCGATCCACGCT
ACGATGACGGCCGGCGAGCTGCACGACCGGCTGAGTGAGGTCGGAGCGAAACTGATGACGGAGGCGATGGCCCGGCTCGA
AGCCGGTGAGCTTCCACTGACGCCGCAACCTCAAGAGGGCGTCGTCTACGCCGCCAAGATCTCGAAGGAAGAGACCCGGA
TCGATTTCTCCCGTCCGGCGGCCGAGGTGCACAATCATATCCGTGGTCTGTCGCCCTTTCCCGGCGCCTGGTTCGAACTT
GATATCGCCGGACGGCGCGAACGCATCAAAGTGCTGGGATCGGAGATATCGCAAGGGGAGGGTGCTCCGGGGCAAGTGCT
GGACGGTTCGCTCGCGATCGCTTGCGGCGAGGGCGCGGTACGCCTGACGAGATTGCAGCGTGCCGGCGGCAAGGCGCTCC
CCGCGGCCGATTTCCTGCGCGGCACGCCGATTGTTGCCAGCGCGGGGATTGGCTGA

Upstream 100 bases:

>100_bases
AACGCGCGGCGCAAAGGCGATCTGATCCTTCGCGTTGCGGCCGCATTCAGCTGGAGCCGCCGCTCGCTCGCGGGCAATCG
AAAGAGACGGAGGCCGTCAC

Downstream 100 bases:

>100_bases
TGCCCCGCTTTCGCCTGATCGTCGAATATGACGGTACCGATTATGTCGGCTGGCAGCGGCAGGACAACGGCCCCTCTGTT
CAAGGAGCGATCGAAAAAGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MPLRIIFMGTPEFSVPTLLALAGAGHEIAAVYTQPPRPGGRRGLDLQKSPVHQAAERLDIPVLTPVNFKEAADRRTFRNF
GADAAVVVAYGLLLPEEILSGTRCGCYNGHASLLPRWRGAAPIQRAIMAGDRETGMMVMKMDKGLDTGPVALARSVPIHA
TMTAGELHDRLSEVGAKLMTEAMARLEAGELPLTPQPQEGVVYAAKISKEETRIDFSRPAAEVHNHIRGLSPFPGAWFEL
DIAGRRERIKVLGSEISQGEGAPGQVLDGSLAIACGEGAVRLTRLQRAGGKALPAADFLRGTPIVASAGIG

Sequences:

>Translated_311_residues
MPLRIIFMGTPEFSVPTLLALAGAGHEIAAVYTQPPRPGGRRGLDLQKSPVHQAAERLDIPVLTPVNFKEAADRRTFRNF
GADAAVVVAYGLLLPEEILSGTRCGCYNGHASLLPRWRGAAPIQRAIMAGDRETGMMVMKMDKGLDTGPVALARSVPIHA
TMTAGELHDRLSEVGAKLMTEAMARLEAGELPLTPQPQEGVVYAAKISKEETRIDFSRPAAEVHNHIRGLSPFPGAWFEL
DIAGRRERIKVLGSEISQGEGAPGQVLDGSLAIACGEGAVRLTRLQRAGGKALPAADFLRGTPIVASAGIG
>Mature_310_residues
PLRIIFMGTPEFSVPTLLALAGAGHEIAAVYTQPPRPGGRRGLDLQKSPVHQAAERLDIPVLTPVNFKEAADRRTFRNFG
ADAAVVVAYGLLLPEEILSGTRCGCYNGHASLLPRWRGAAPIQRAIMAGDRETGMMVMKMDKGLDTGPVALARSVPIHAT
MTAGELHDRLSEVGAKLMTEAMARLEAGELPLTPQPQEGVVYAAKISKEETRIDFSRPAAEVHNHIRGLSPFPGAWFELD
IAGRRERIKVLGSEISQGEGAPGQVLDGSLAIACGEGAVRLTRLQRAGGKALPAADFLRGTPIVASAGIG

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=318, Percent_Identity=29.874213836478, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI21614513, Length=319, Percent_Identity=27.8996865203762, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI164663775, Length=328, Percent_Identity=28.6585365853659, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1789683, Length=303, Percent_Identity=43.2343234323432, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788589, Length=275, Percent_Identity=27.6363636363636, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI133930964, Length=328, Percent_Identity=27.4390243902439, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319458, Length=291, Percent_Identity=26.8041237113402, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45550868, Length=319, Percent_Identity=31.0344827586207, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585660, Length=328, Percent_Identity=29.2682926829268, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28571984, Length=231, Percent_Identity=33.7662337662338, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_SINMW (A6U5I5)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001325750.1
- ProteinModelPortal:   A6U5I5
- SMR:   A6U5I5
- STRING:   A6U5I5
- GeneID:   5320882
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_0055
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- BioCyc:   SMED366394:SMED_0055-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33046; Mature: 32914

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRIIFMGTPEFSVPTLLALAGAGHEIAAVYTQPPRPGGRRGLDLQKSPVHQAAERLDI
CCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCC
PVLTPVNFKEAADRRTFRNFGADAAVVVAYGLLLPEEILSGTRCGCYNGHASLLPRWRGA
CEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHCCCCCCCCCCCHHHCCCCCCC
APIQRAIMAGDRETGMMVMKMDKGLDTGPVALARSVPIHATMTAGELHDRLSEVGAKLMT
CHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHCCCCEEEEECHHHHHHHHHHHHHHHHH
EAMARLEAGELPLTPQPQEGVVYAAKISKEETRIDFSRPAAEVHNHIRGLSPFPGAWFEL
HHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCHHHHHHHHCCCCCCCCCEEEE
DIAGRRERIKVLGSEISQGEGAPGQVLDGSLAIACGEGAVRLTRLQRAGGKALPAADFLR
EECCCHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHC
GTPIVASAGIG
CCCEEECCCCC
>Mature Secondary Structure 
PLRIIFMGTPEFSVPTLLALAGAGHEIAAVYTQPPRPGGRRGLDLQKSPVHQAAERLDI
CEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCC
PVLTPVNFKEAADRRTFRNFGADAAVVVAYGLLLPEEILSGTRCGCYNGHASLLPRWRGA
CEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHCCCCCCCCCCCHHHCCCCCCC
APIQRAIMAGDRETGMMVMKMDKGLDTGPVALARSVPIHATMTAGELHDRLSEVGAKLMT
CHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHCCCCEEEEECHHHHHHHHHHHHHHHHH
EAMARLEAGELPLTPQPQEGVVYAAKISKEETRIDFSRPAAEVHNHIRGLSPFPGAWFEL
HHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCHHHHHHHHCCCCCCCCCEEEE
DIAGRRERIKVLGSEISQGEGAPGQVLDGSLAIACGEGAVRLTRLQRAGGKALPAADFLR
EECCCHHHHHHHHHHHHCCCCCCCCEECCCEEEEECCCCHHHHHHHHCCCCCCCHHHHHC
GTPIVASAGIG
CCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA