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The map label for this gene is truA [H]

Identifier: 150395284

GI number: 150395284

Start: 58914

End: 59657

Strand: Direct

Name: truA [H]

Synonym: Smed_0056

Alternate gene names: 150395284

Gene position: 58914-59657 (Clockwise)

Preceding gene: 150395283

Following gene: 150395287

Centisome position: 1.56

GC content: 64.11

Gene sequence:

>744_bases
ATGCCCCGCTTTCGCCTGATCGTCGAATATGACGGTACCGATTATGTCGGCTGGCAGCGGCAGGACAACGGCCCCTCTGT
TCAAGGAGCGATCGAAAAAGCGGTCCTGTCGCTTACGGGCGAGACGGTCCGGGTCCGCGGCGCAGGGCGAACCGATTCCG
GCGTTCACGCCAGGGGTCAGGTGGCTCATCTCGATCTAACCCGCGCGTGGAAGCCGTACACGCTTCAAAACGCCCTCAAC
GCGCATCTGTCGCTTGCCGGCGAGCGCGTTTCGATCCTGGAGGTCGAGGAAGCCGCCGCCGATTTCGACGCGCGTTTCTC
GGCCATCCGCCGACACTATCTCTATCGGATCATTTCCCGCCGATCACCGCTGGCGCTTGAGGCGCGCCGTGCCTGGTGGG
TGCCGAAGGCGCTTGATCATGACGCAATGCACGAGGCAGCGCAGCGCCTCTTGGGCCATCACGACTTCACGACCTTCCGC
TCTGCCAACTGCCAGGCGACGAGCCCGATGCGCACGCTGGACCGGCTGGACGTCACGCGCACGGGCGACCTGATCGAAAT
CCGCGCCAGCGCGCAAAGCTTCCTTCATAACCAGATCCGTTCTTTCGCGGGCTCCTTGAAGCTGGTCGGCGAGAGCAAAT
GGACGCCGGACAACCTCCAAGCCGCGCTCGAGGCGCGGGACCGCAAAGCCTGCGGTCCGGTCGCCCCGCCGGACGGACTT
TATTTCATGCGCGTCGATTACTGA

Upstream 100 bases:

>100_bases
CGGTACGCCTGACGAGATTGCAGCGTGCCGGCGGCAAGGCGCTCCCCGCGGCCGATTTCCTGCGCGGCACGCCGATTGTT
GCCAGCGCGGGGATTGGCTG

Downstream 100 bases:

>100_bases
CGCCGAGATCACGATGATTTTCGGGAGGCGGCGGAAAAACGCAGACGGTTTTCCTCATCCCGCTCTGAATTTCTCCCGCT
CGCGCTCATAGTAACGCCGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MPRFRLIVEYDGTDYVGWQRQDNGPSVQGAIEKAVLSLTGETVRVRGAGRTDSGVHARGQVAHLDLTRAWKPYTLQNALN
AHLSLAGERVSILEVEEAAADFDARFSAIRRHYLYRIISRRSPLALEARRAWWVPKALDHDAMHEAAQRLLGHHDFTTFR
SANCQATSPMRTLDRLDVTRTGDLIEIRASAQSFLHNQIRSFAGSLKLVGESKWTPDNLQAALEARDRKACGPVAPPDGL
YFMRVDY

Sequences:

>Translated_247_residues
MPRFRLIVEYDGTDYVGWQRQDNGPSVQGAIEKAVLSLTGETVRVRGAGRTDSGVHARGQVAHLDLTRAWKPYTLQNALN
AHLSLAGERVSILEVEEAAADFDARFSAIRRHYLYRIISRRSPLALEARRAWWVPKALDHDAMHEAAQRLLGHHDFTTFR
SANCQATSPMRTLDRLDVTRTGDLIEIRASAQSFLHNQIRSFAGSLKLVGESKWTPDNLQAALEARDRKACGPVAPPDGL
YFMRVDY
>Mature_246_residues
PRFRLIVEYDGTDYVGWQRQDNGPSVQGAIEKAVLSLTGETVRVRGAGRTDSGVHARGQVAHLDLTRAWKPYTLQNALNA
HLSLAGERVSILEVEEAAADFDARFSAIRRHYLYRIISRRSPLALEARRAWWVPKALDHDAMHEAAQRLLGHHDFTTFRS
ANCQATSPMRTLDRLDVTRTGDLIEIRASAQSFLHNQIRSFAGSLKLVGESKWTPDNLQAALEARDRKACGPVAPPDGLY
FMRVDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=36.1313868613139, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=38.3673469387755, Blast_Score=162, Evalue=2e-41,
Organism=Drosophila melanogaster, GI116007752, Length=304, Percent_Identity=28.6184210526316, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27735; Mature: 27603

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFRLIVEYDGTDYVGWQRQDNGPSVQGAIEKAVLSLTGETVRVRGAGRTDSGVHARGQ
CCCEEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCE
VAHLDLTRAWKPYTLQNALNAHLSLAGERVSILEVEEAAADFDARFSAIRRHYLYRIISR
EEEEEHHHCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
RSPLALEARRAWWVPKALDHDAMHEAAQRLLGHHDFTTFRSANCQATSPMRTLDRLDVTR
CCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHCCCCC
TGDLIEIRASAQSFLHNQIRSFAGSLKLVGESKWTPDNLQAALEARDRKACGPVAPPDGL
CCCEEEEHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
YFMRVDY
EEEEECC
>Mature Secondary Structure 
PRFRLIVEYDGTDYVGWQRQDNGPSVQGAIEKAVLSLTGETVRVRGAGRTDSGVHARGQ
CCEEEEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCE
VAHLDLTRAWKPYTLQNALNAHLSLAGERVSILEVEEAAADFDARFSAIRRHYLYRIISR
EEEEEHHHCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
RSPLALEARRAWWVPKALDHDAMHEAAQRLLGHHDFTTFRSANCQATSPMRTLDRLDVTR
CCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHCCCCC
TGDLIEIRASAQSFLHNQIRSFAGSLKLVGESKWTPDNLQAALEARDRKACGPVAPPDGL
CCCEEEEHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
YFMRVDY
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]