Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 150395282

GI number: 150395282

Start: 57379

End: 57903

Strand: Direct

Name: def [H]

Synonym: Smed_0054

Alternate gene names: 150395282

Gene position: 57379-57903 (Clockwise)

Preceding gene: 150395278

Following gene: 150395283

Centisome position: 1.52

GC content: 59.24

Gene sequence:

>525_bases
ATGACGATCAAGCCGCTCATCATTCTTCCCGATCCGGTTCTGCGCCAGGTGTCGACACCCGTGGAAACCGTTGACGCCGA
TATTCGCCGGCTCGCCGACGATATGCTGGAAACCATGTACGATGCGCCCGGTATCGGGCTCGCCGCGATACAGATCGGCG
TGCCGAAGCGCCTCCTGGTGCTCGACGTGACGAAGGAGGGAGAGGAGAAGCAGCCGCTCGTCTTCATCAATCCGAAGGTC
GTTCGCTCTTCCGAGGAGCGTTCGGTCTATGAGGAGGGCTGCCTTTCGATCCCCGACTACTACGCCGAGGTCGAACGGCC
GGCCGCGATCACTGTCGAGTACGTCGACCGTGAAGGCAAGGAACAGGCAGTTGAGGCCGATGGCTTGCTCGCTACCTGCC
TGCAGCACGAGATCGACCATCTGAACGGTGTCCTGTTCATCGATTACATTTCAAAGCTCAAGCGCGATATGGTGATTCGC
AAGTTCACAAAGGCGGCGAAAACGCGCGGCGCAAAGGCGATCTGA

Upstream 100 bases:

>100_bases
AAAACGGGAACAAACAAGATTGTGCCCACAATCAGGCCGGAGACCAGGGGTACGGCAAATTTCTTGCGTGTCGACGGAAA
GATCGGCTATGGGGATCGAC

Downstream 100 bases:

>100_bases
TCCTTCGCGTTGCGGCCGCATTCAGCTGGAGCCGCCGCTCGCTCGCGGGCAATCGAAAGAGACGGAGGCCGTCACTTGCC
GCTTCGCATCATCTTCATGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTIKPLIILPDPVLRQVSTPVETVDADIRRLADDMLETMYDAPGIGLAAIQIGVPKRLLVLDVTKEGEEKQPLVFINPKV
VRSSEERSVYEEGCLSIPDYYAEVERPAAITVEYVDREGKEQAVEADGLLATCLQHEIDHLNGVLFIDYISKLKRDMVIR
KFTKAAKTRGAKAI

Sequences:

>Translated_174_residues
MTIKPLIILPDPVLRQVSTPVETVDADIRRLADDMLETMYDAPGIGLAAIQIGVPKRLLVLDVTKEGEEKQPLVFINPKV
VRSSEERSVYEEGCLSIPDYYAEVERPAAITVEYVDREGKEQAVEADGLLATCLQHEIDHLNGVLFIDYISKLKRDMVIR
KFTKAAKTRGAKAI
>Mature_173_residues
TIKPLIILPDPVLRQVSTPVETVDADIRRLADDMLETMYDAPGIGLAAIQIGVPKRLLVLDVTKEGEEKQPLVFINPKVV
RSSEERSVYEEGCLSIPDYYAEVERPAAITVEYVDREGKEQAVEADGLLATCLQHEIDHLNGVLFIDYISKLKRDMVIRK
FTKAAKTRGAKAI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=155, Percent_Identity=34.1935483870968, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=45.8823529411765, Blast_Score=153, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24645728, Length=179, Percent_Identity=32.4022346368715, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=28.3018867924528, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19421; Mature: 19290

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKPLIILPDPVLRQVSTPVETVDADIRRLADDMLETMYDAPGIGLAAIQIGVPKRLLV
CCCCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
LDVTKEGEEKQPLVFINPKVVRSSEERSVYEEGCLSIPDYYAEVERPAAITVEYVDREGK
EEECCCCCCCCCEEEECHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCH
EQAVEADGLLATCLQHEIDHLNGVLFIDYISKLKRDMVIRKFTKAAKTRGAKAI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TIKPLIILPDPVLRQVSTPVETVDADIRRLADDMLETMYDAPGIGLAAIQIGVPKRLLV
CCCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
LDVTKEGEEKQPLVFINPKVVRSSEERSVYEEGCLSIPDYYAEVERPAAITVEYVDREGK
EEECCCCCCCCCEEEECHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCEEEEEECCCCCH
EQAVEADGLLATCLQHEIDHLNGVLFIDYISKLKRDMVIRKFTKAAKTRGAKAI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]