Click here to switch to the map view.

The map label for this gene is rihA [H]

Identifier: 150395278

GI number: 150395278

Start: 51351

End: 52295

Strand: Direct

Name: rihA [H]

Synonym: Smed_0050

Alternate gene names: 150395278

Gene position: 51351-52295 (Clockwise)

Preceding gene: 150395275

Following gene: 150395282

Centisome position: 1.36

GC content: 62.43

Gene sequence:

>945_bases
GTGTCAAGCGCGAGAAAAATCATCATCGACACGGATCCGGGTCAGGACGACGCAGCGGCAATCATGCTCGCTTTCGGCAG
CCCGGAAGAAATCGACGTCCTCGGCATCACCACCGTCGCCGGCAATGTCCCGCTGACGCTGACGGCGCGCAATGCTCGGA
TAGTCTGCGAGCTTTGCAACCGGACCGACGTCCGGATCTTCGCAGGCTCCGAGCGGCCGGTCGCCCGCCCGCTTGTCACC
GCCGAGCATGTTCACGGCAAGACGGGGCTCGACGGTCCTCAACTCGACGAGCCGACGATGCCGATTCAGGAGCAACACGC
CGTCGATTTCATCGTCGAAACGCTGCGTGAGGAGGCACCGGGTGCCGTCACGCTTTGTACGCTCGGTCCGCTTACGAACA
TCGCGCTGGCCTTGACGAAGGCACCGGAGATCGCGCCGGCGGTTCGCGAGCTCGTGATGATGGGCGGCGGCTTCTTCGAG
GGCGGCAACATAACGCCGGCGGCGGAATTCAACATCTATGTCGACCCGGAGGCAGCCGAGATCGTCTTCCGCTCCGGCAT
CCCCATAGTGATGATGCCTCTCGACGTTACCCATCGCGTTCTCACACGGAAGGCGCGCGTTGAGAAGATTCGCGACATCG
GCAGCCCGGCGGCAGTCGCCCTTGCGGAAATGCTCGAATTCTTCGAGCGGTTCGACATCGAAAAATACGGCACCGATGGC
GGGCCGCTGCATGATCCGACGGTGATTGCCTATCTGCTGAGGCCCGAGCTTTTCACCGGCCGGGACTGCAATGTCGAGAT
CGAAACGGCGTCGCCGCTCACCACCGGTATGACCGTGGTCGACTGGTGGCAGGTCACCGGCCGCGCGCACAATGCCAAAG
TCATGCGGCACATCGACGACGAGGGTTTTTTCGAGCTTTTGACCGAACGCATCGCGCGTATCTGA

Upstream 100 bases:

>100_bases
ATATGGTGGCTGTGTGCAATGAGTGCAAGCCGCTTGCCTAGGCGCCCACGCAAATCATAGTCTCTCACTCGAGAGCGATG
GTCCGGCAGGGAGATGAAAA

Downstream 100 bases:

>100_bases
GAGGCAAGCACGCCCGTCTGGGAGATGATGACGGCGCCCGATGAGTTCAGGCGCCGTTTGGAAGTCCGAAGCCGTTGGGG
ATCAGAATTCTTCCCAATTG

Product: inosine/uridine-preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSSARKIIIDTDPGQDDAAAIMLAFGSPEEIDVLGITTVAGNVPLTLTARNARIVCELCNRTDVRIFAGSERPVARPLVT
AEHVHGKTGLDGPQLDEPTMPIQEQHAVDFIVETLREEAPGAVTLCTLGPLTNIALALTKAPEIAPAVRELVMMGGGFFE
GGNITPAAEFNIYVDPEAAEIVFRSGIPIVMMPLDVTHRVLTRKARVEKIRDIGSPAAVALAEMLEFFERFDIEKYGTDG
GPLHDPTVIAYLLRPELFTGRDCNVEIETASPLTTGMTVVDWWQVTGRAHNAKVMRHIDDEGFFELLTERIARI

Sequences:

>Translated_314_residues
MSSARKIIIDTDPGQDDAAAIMLAFGSPEEIDVLGITTVAGNVPLTLTARNARIVCELCNRTDVRIFAGSERPVARPLVT
AEHVHGKTGLDGPQLDEPTMPIQEQHAVDFIVETLREEAPGAVTLCTLGPLTNIALALTKAPEIAPAVRELVMMGGGFFE
GGNITPAAEFNIYVDPEAAEIVFRSGIPIVMMPLDVTHRVLTRKARVEKIRDIGSPAAVALAEMLEFFERFDIEKYGTDG
GPLHDPTVIAYLLRPELFTGRDCNVEIETASPLTTGMTVVDWWQVTGRAHNAKVMRHIDDEGFFELLTERIARI
>Mature_313_residues
SSARKIIIDTDPGQDDAAAIMLAFGSPEEIDVLGITTVAGNVPLTLTARNARIVCELCNRTDVRIFAGSERPVARPLVTA
EHVHGKTGLDGPQLDEPTMPIQEQHAVDFIVETLREEAPGAVTLCTLGPLTNIALALTKAPEIAPAVRELVMMGGGFFEG
GNITPAAEFNIYVDPEAAEIVFRSGIPIVMMPLDVTHRVLTRKARVEKIRDIGSPAAVALAEMLEFFERFDIEKYGTDGG
PLHDPTVIAYLLRPELFTGRDCNVEIETASPLTTGMTVVDWWQVTGRAHNAKVMRHIDDEGFFELLTERIARI

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=49.8381877022654, Blast_Score=280, Evalue=7e-77,
Organism=Escherichia coli, GI1788486, Length=307, Percent_Identity=42.3452768729642, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1786213, Length=306, Percent_Identity=39.2156862745098, Blast_Score=164, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI32565474, Length=200, Percent_Identity=34.5, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17565698, Length=293, Percent_Identity=28.3276450511945, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320608, Length=266, Percent_Identity=30.8270676691729, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24641837, Length=330, Percent_Identity=29.6969696969697, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24583915, Length=333, Percent_Identity=26.7267267267267, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24641839, Length=317, Percent_Identity=29.0220820189274, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 34223; Mature: 34092

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSARKIIIDTDPGQDDAAAIMLAFGSPEEIDVLGITTVAGNVPLTLTARNARIVCELCN
CCCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCEEEEEECC
RTDVRIFAGSERPVARPLVTAEHVHGKTGLDGPQLDEPTMPIQEQHAVDFIVETLREEAP
CCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GAVTLCTLGPLTNIALALTKAPEIAPAVRELVMMGGGFFEGGNITPAAEFNIYVDPEAAE
CEEEEEECCCHHHHHEEEECCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEEECCCHHE
IVFRSGIPIVMMPLDVTHRVLTRKARVEKIRDIGSPAAVALAEMLEFFERFDIEKYGTDG
EEEECCCCEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCC
GPLHDPTVIAYLLRPELFTGRDCNVEIETASPLTTGMTVVDWWQVTGRAHNAKVMRHIDD
CCCCCHHHEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEHHHCCCCCCHHHHHHCCC
EGFFELLTERIARI
CHHHHHHHHHHHCC
>Mature Secondary Structure 
SSARKIIIDTDPGQDDAAAIMLAFGSPEEIDVLGITTVAGNVPLTLTARNARIVCELCN
CCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCEEEEEECC
RTDVRIFAGSERPVARPLVTAEHVHGKTGLDGPQLDEPTMPIQEQHAVDFIVETLREEAP
CCCEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GAVTLCTLGPLTNIALALTKAPEIAPAVRELVMMGGGFFEGGNITPAAEFNIYVDPEAAE
CEEEEEECCCHHHHHEEEECCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEEEECCCHHE
IVFRSGIPIVMMPLDVTHRVLTRKARVEKIRDIGSPAAVALAEMLEFFERFDIEKYGTDG
EEEECCCCEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCC
GPLHDPTVIAYLLRPELFTGRDCNVEIETASPLTTGMTVVDWWQVTGRAHNAKVMRHIDD
CCCCCHHHEEEECCCCCCCCCCCCEEEECCCCCCCCCEEEEEHHHCCCCCCHHHHHHCCC
EGFFELLTERIARI
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA