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The map label for this gene is tfb

Identifier: 150401578

GI number: 150401578

Start: 1212916

End: 1213920

Strand: Reverse

Name: tfb

Synonym: Maeo_1155

Alternate gene names: 150401578

Gene position: 1213920-1212916 (Counterclockwise)

Preceding gene: 150401579

Following gene: 150401577

Centisome position: 77.34

GC content: 37.91

Gene sequence:

>1005_bases
ATGGATATTAAACTAATATCAAAAAAGAAAAATATTGAAGAAAGAAAACAATTATTAAGTGAATTGCATGCATCTAAAAG
TGTCATTGTCGAAAAAGACGAAGAGCTAGTATGCCCTATGTGCGATAGTAAAAACATAATTAAAGATTATGAAAAGGCAG
AAATCGTATGCGAAGATTGTGGATGTGTTTTACAGGACAATTTATTTGATGTTGGTCCAGAATGGAGAGCATTTGACCAC
GAACAAAGGGTTAAGAGAAGTAGGGTAGGAGCTCCAATGACATACACAATCCATGATAAAGGATTATCCACAGTGATAGA
TTGGAGAAATAAAGATAGCTACGGGAAGGACATTTCGGCCGATAAAAGGGCACAATTATATAGATTAAGGAAATGGCAAA
GAAGAATTAGGGTATCAGATGCGTCGGAACGAAATTTAGCATTTGCATTGTCGGAATTAGATAGAATTGCTTCAAAATTA
GGATTACCTAGAAATGTTAGGGAAAATGCTGCGGTTTTATACCGGGGAGCTGTGGAAAAAGGACTAATTAGGGGAAGGAG
TATTGAAGGAGTAGCTGCGGCTGCATTATATGCAGCATGTAGAAGATGTAAAGTCCCAAGAACGCTGGACGAAATTGCAG
AAATGTCCAGAGTAGATAGGAAAGAAATAGGTAGGACATACAGATTTATATCCCGAGAATTAAAAATAAGGTTGGCCCCA
ACAAGCCCAATTGATTATGTGCCAAGATTTGCATCCGAACTAAAATTACCAGGGGAAGTTGAATCAAAAGCCATATCCAT
ACTTCAAAAAGCAGGCGATAAAGGATTAACCAGTGGGAGGGGACCAACAGGGGTTGCAGCTGCTGCCATATATATTGCAA
GTGTTTTACATGGAACAAGAAAGACCCAAAGAGAAGTCGCAGATGTTGCAGGGGTTACCGAAGTCACCATAAGGAACAGA
TATAAAGAATTAACCGAACATTTAGATATTGATGTAACATTATAA

Upstream 100 bases:

>100_bases
GCTTCGCTTCTATCCGATAATTTCCCTTGGAAATTATTGGGTAAATTCAATGTTATAATTTATTCACTATGCCCCATATA
TTTGGCTTCTTGGTGTTATT

Downstream 100 bases:

>100_bases
ACCCATATATACAATAATTCATATGGTAGTGATAATATGGGACTATTTGATAAATCAAAAAAACCAACATCCAGAACATT
AAATAAAAGTAAAGTAAGTA

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MDIKLISKKKNIEERKQLLSELHASKSVIVEKDEELVCPMCDSKNIIKDYEKAEIVCEDCGCVLQDNLFDVGPEWRAFDH
EQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYGKDISADKRAQLYRLRKWQRRIRVSDASERNLAFALSELDRIASKL
GLPRNVRENAAVLYRGAVEKGLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEMSRVDRKEIGRTYRFISRELKIRLAP
TSPIDYVPRFASELKLPGEVESKAISILQKAGDKGLTSGRGPTGVAAAAIYIASVLHGTRKTQREVADVAGVTEVTIRNR
YKELTEHLDIDVTL

Sequences:

>Translated_334_residues
MDIKLISKKKNIEERKQLLSELHASKSVIVEKDEELVCPMCDSKNIIKDYEKAEIVCEDCGCVLQDNLFDVGPEWRAFDH
EQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYGKDISADKRAQLYRLRKWQRRIRVSDASERNLAFALSELDRIASKL
GLPRNVRENAAVLYRGAVEKGLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEMSRVDRKEIGRTYRFISRELKIRLAP
TSPIDYVPRFASELKLPGEVESKAISILQKAGDKGLTSGRGPTGVAAAAIYIASVLHGTRKTQREVADVAGVTEVTIRNR
YKELTEHLDIDVTL
>Mature_334_residues
MDIKLISKKKNIEERKQLLSELHASKSVIVEKDEELVCPMCDSKNIIKDYEKAEIVCEDCGCVLQDNLFDVGPEWRAFDH
EQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYGKDISADKRAQLYRLRKWQRRIRVSDASERNLAFALSELDRIASKL
GLPRNVRENAAVLYRGAVEKGLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEMSRVDRKEIGRTYRFISRELKIRLAP
TSPIDYVPRFASELKLPGEVESKAISILQKAGDKGLTSGRGPTGVAAAAIYIASVLHGTRKTQREVADVAGVTEVTIRNR
YKELTEHLDIDVTL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=288, Percent_Identity=36.4583333333333, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI22035556, Length=302, Percent_Identity=28.476821192053, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17564892, Length=288, Percent_Identity=36.1111111111111, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17531397, Length=293, Percent_Identity=23.5494880546075, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325343, Length=310, Percent_Identity=29.3548387096774, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6321685, Length=300, Percent_Identity=24, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136758, Length=287, Percent_Identity=35.191637630662, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI281361961, Length=302, Percent_Identity=26.8211920529801, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24647658, Length=302, Percent_Identity=26.8211920529801, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B_META3 (A6UW60)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001325344.1
- ProteinModelPortal:   A6UW60
- STRING:   A6UW60
- GeneID:   5327478
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_1155
- eggNOG:   arNOG04485
- HOGENOM:   HBG646536
- OMA:   HDYERAE
- ProtClustDB:   PRK00423
- BioCyc:   MAEO419665:MAEO_1155-MONOMER
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 37655; Mature: 37655

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKLISKKKNIEERKQLLSELHASKSVIVEKDEELVCPMCDSKNIIKDYEKAEIVCEDC
CCCEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHC
GCVLQDNLFDVGPEWRAFDHEQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYGKDISA
CCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCC
DKRAQLYRLRKWQRRIRVSDASERNLAFALSELDRIASKLGLPRNVRENAAVLYRGAVEK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHH
GLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEMSRVDRKEIGRTYRFISRELKIRLAP
HHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
TSPIDYVPRFASELKLPGEVESKAISILQKAGDKGLTSGRGPTGVAAAAIYIASVLHGTR
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KTQREVADVAGVTEVTIRNRYKELTEHLDIDVTL
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEC
>Mature Secondary Structure
MDIKLISKKKNIEERKQLLSELHASKSVIVEKDEELVCPMCDSKNIIKDYEKAEIVCEDC
CCCEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHC
GCVLQDNLFDVGPEWRAFDHEQRVKRSRVGAPMTYTIHDKGLSTVIDWRNKDSYGKDISA
CCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCC
DKRAQLYRLRKWQRRIRVSDASERNLAFALSELDRIASKLGLPRNVRENAAVLYRGAVEK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHH
GLIRGRSIEGVAAAALYAACRRCKVPRTLDEIAEMSRVDRKEIGRTYRFISRELKIRLAP
HHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
TSPIDYVPRFASELKLPGEVESKAISILQKAGDKGLTSGRGPTGVAAAAIYIASVLHGTR
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KTQREVADVAGVTEVTIRNRYKELTEHLDIDVTL
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA