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The map label for this gene is pfkA3 [H]

Identifier: 150008153

GI number: 150008153

Start: 1777430

End: 1779073

Strand: Reverse

Name: pfkA3 [H]

Synonym: BDI_1519

Alternate gene names: 150008153

Gene position: 1779073-1777430 (Counterclockwise)

Preceding gene: 150008154

Following gene: 150008152

Centisome position: 36.98

GC content: 50.24

Gene sequence:

>1644_bases
ATGACAAAGAGTGCATTACAAATCGCAAGAGCAGCTTACCAACCGAAGGTACCCGCTGCGTTGAAAGGTGCAGTGAAAGC
CGTTGACGGCGAGTATACTCAATCAGTAGCCGATCAGGAAGAAATCAAGAAATTGTTCCCGAACACTTACGGAATGCCGA
TCGTTACATTCGAGAAGAGTAACGAGGAGAAGGCTATGCCTGTCATGAACGTTGGTGTGATCCTTTCCGGTGGTCAGGCT
CCCGGTGGTCACAACGTGATCGCTGGTTTGTTCGACGGTATCAAGGCTCACAACGCAGATTCTCGTCTGTATGGTTTCAT
CTTGGGCCCGGGCGGTTTGATCGATCATAAATATATGGAATTGACGGCTGATATTATCGACGAGTACCGTAACACGGGCG
GTTTCGATATGATCGGTTCCGGACGTACGAAATTAGAGACGAAAGAGCAATTCGATAAAGGTCTTCAGATCTTGAAAGAG
CTTGATATCAAGGCTTTGGTTATTATCGGCGGTGACGATTCTAACACGAACGCTTGCGTATTGGCCGAGTATTACAAGGC
TATTGGCGCCGGCGTACAGGTAATCGGTTGCCCGAAGACGATCGACGGCGACTTGAAGAACGCCCAGATCGAGACCTCTT
TCGGTTTCGACACGGCTTGTAAGGTTTACTCCGAGGTAATCGGTAACATCCAACGTGACTGTAACTCCGCCCAGAAATAC
TGGCACTTCATCAAATTGATGGGTCGCTCCGCTTCTCATATCGCTTTGGAGTGCGCTTTGCAGACTCAACCGAACGTTTG
TATCATCTCCGAGGAGGTTGAGGAGAAGAATATGTCTTTGGACGATATCGTTACTTACGTGGCTGGTATCGTGGCTAAAC
GTGCCGCCGAGGGTAATAACTTCGGTACGGTATTGATCCCTGAGGGCTTGATCGAGTTCGTTCCGGCTATGAAACGCTTG
ATCGCCGAGTTGAACGACTTCTTGGCTAAGCACGACGCTGAGTTCAAGATGATCAAGAAGAGCGAGCAACGTGCTTATAT
CATCAGCAAGTTGACGAAGGAGAACTCTGATCTGTATGCTTCTCTTCCGGAGGGCGTAGCTCGTCAGTTGTCTTTGGACC
GTGACCCGCACGGAAACGTACAGGTTTCTTTGATCGAGACTGAGAAGTTATTGTCTGAGATGGTTGGCAAGAAATTGGCT
GAGTGGAAGGCCGAGGGCAAGTACGTAGGCAAGTTCGCCGCACAACACCACTTCTTCGGTTACGAGGGTCGTTGCGCCGC
TCCGTCTAACTACGATGCGGACTACTGCTACTCTTTGGGTTACACGGCATCTTGCTTGATCGCCGCCGGTAAGACAGGTT
ATATGTCTTCCGTACGTAACACGACCGCTCCCGCTGATCAATGGATCGCCGGTGGTATCCCTGTTACTATGATGATGAAC
ATGGAGAAACGTCATGGCGAGATGAAGCCGGTTATCCAGAAGGCTTTGGTTAAGCTTGACGGCGCTCCTTTCAAGGCGTT
CGCCGCTAACCGTGACGAGTGGGCTTTGACTACTAGCTATGTATATCCGGGTCCGATCCAGTACTTCGGTCCGACGGAGG
TTTGCGACCAGCCGACTAAGACTTTGCAGTTGGAGCAAGCTTAA

Upstream 100 bases:

>100_bases
AACATATCCCCTTGTCTTTTTATTAATACGGATTTTTTTCCTTATATTCGCGTCACTTTTGAACACGTATCAAATAATTT
CTAAATAAATCAATTAAGAA

Downstream 100 bases:

>100_bases
TACAGACTTATACATTATATAATATATAAGCTAGAGAGGATGTCATTTATTTGGCATCCTCTTTTTATTTACATACATTT
GCCACGAGATCTTAAACCTA

Product: diphosphate--fructose-6-phosphate 1-phosphotransferase

Products: NA

Alternate protein names: Phosphofructokinase 3; Phosphohexokinase 3 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTKSALQIARAAYQPKVPAALKGAVKAVDGEYTQSVADQEEIKKLFPNTYGMPIVTFEKSNEEKAMPVMNVGVILSGGQA
PGGHNVIAGLFDGIKAHNADSRLYGFILGPGGLIDHKYMELTADIIDEYRNTGGFDMIGSGRTKLETKEQFDKGLQILKE
LDIKALVIIGGDDSNTNACVLAEYYKAIGAGVQVIGCPKTIDGDLKNAQIETSFGFDTACKVYSEVIGNIQRDCNSAQKY
WHFIKLMGRSASHIALECALQTQPNVCIISEEVEEKNMSLDDIVTYVAGIVAKRAAEGNNFGTVLIPEGLIEFVPAMKRL
IAELNDFLAKHDAEFKMIKKSEQRAYIISKLTKENSDLYASLPEGVARQLSLDRDPHGNVQVSLIETEKLLSEMVGKKLA
EWKAEGKYVGKFAAQHHFFGYEGRCAAPSNYDADYCYSLGYTASCLIAAGKTGYMSSVRNTTAPADQWIAGGIPVTMMMN
MEKRHGEMKPVIQKALVKLDGAPFKAFAANRDEWALTTSYVYPGPIQYFGPTEVCDQPTKTLQLEQA

Sequences:

>Translated_547_residues
MTKSALQIARAAYQPKVPAALKGAVKAVDGEYTQSVADQEEIKKLFPNTYGMPIVTFEKSNEEKAMPVMNVGVILSGGQA
PGGHNVIAGLFDGIKAHNADSRLYGFILGPGGLIDHKYMELTADIIDEYRNTGGFDMIGSGRTKLETKEQFDKGLQILKE
LDIKALVIIGGDDSNTNACVLAEYYKAIGAGVQVIGCPKTIDGDLKNAQIETSFGFDTACKVYSEVIGNIQRDCNSAQKY
WHFIKLMGRSASHIALECALQTQPNVCIISEEVEEKNMSLDDIVTYVAGIVAKRAAEGNNFGTVLIPEGLIEFVPAMKRL
IAELNDFLAKHDAEFKMIKKSEQRAYIISKLTKENSDLYASLPEGVARQLSLDRDPHGNVQVSLIETEKLLSEMVGKKLA
EWKAEGKYVGKFAAQHHFFGYEGRCAAPSNYDADYCYSLGYTASCLIAAGKTGYMSSVRNTTAPADQWIAGGIPVTMMMN
MEKRHGEMKPVIQKALVKLDGAPFKAFAANRDEWALTTSYVYPGPIQYFGPTEVCDQPTKTLQLEQA
>Mature_546_residues
TKSALQIARAAYQPKVPAALKGAVKAVDGEYTQSVADQEEIKKLFPNTYGMPIVTFEKSNEEKAMPVMNVGVILSGGQAP
GGHNVIAGLFDGIKAHNADSRLYGFILGPGGLIDHKYMELTADIIDEYRNTGGFDMIGSGRTKLETKEQFDKGLQILKEL
DIKALVIIGGDDSNTNACVLAEYYKAIGAGVQVIGCPKTIDGDLKNAQIETSFGFDTACKVYSEVIGNIQRDCNSAQKYW
HFIKLMGRSASHIALECALQTQPNVCIISEEVEEKNMSLDDIVTYVAGIVAKRAAEGNNFGTVLIPEGLIEFVPAMKRLI
AELNDFLAKHDAEFKMIKKSEQRAYIISKLTKENSDLYASLPEGVARQLSLDRDPHGNVQVSLIETEKLLSEMVGKKLAE
WKAEGKYVGKFAAQHHFFGYEGRCAAPSNYDADYCYSLGYTASCLIAAGKTGYMSSVRNTTAPADQWIAGGIPVTMMMNM
EKRHGEMKPVIQKALVKLDGAPFKAFAANRDEWALTTSYVYPGPIQYFGPTEVCDQPTKTLQLEQA

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=291, Percent_Identity=27.4914089347079, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 59833; Mature: 59702

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSALQIARAAYQPKVPAALKGAVKAVDGEYTQSVADQEEIKKLFPNTYGMPIVTFEKS
CCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHCCCCCCCEEEEEECC
NEEKAMPVMNVGVILSGGQAPGGHNVIAGLFDGIKAHNADSRLYGFILGPGGLIDHKYME
CCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHH
LTADIIDEYRNTGGFDMIGSGRTKLETKEQFDKGLQILKELDIKALVIIGGDDSNTNACV
HHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEE
LAEYYKAIGAGVQVIGCPKTIDGDLKNAQIETSFGFDTACKVYSEVIGNIQRDCNSAQKY
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHFIKLMGRSASHIALECALQTQPNVCIISEEVEEKNMSLDDIVTYVAGIVAKRAAEGNN
HHHHHHHCCCCCEEEEEEEECCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
FGTVLIPEGLIEFVPAMKRLIAELNDFLAKHDAEFKMIKKSEQRAYIISKLTKENSDLYA
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCEEE
SLPEGVARQLSLDRDPHGNVQVSLIETEKLLSEMVGKKLAEWKAEGKYVGKFAAQHHFFG
ECHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
YEGRCAAPSNYDADYCYSLGYTASCLIAAGKTGYMSSVRNTTAPADQWIAGGIPVTMMMN
CCCCCCCCCCCCCHHHEECCCCEEEEEECCCCHHHHHHCCCCCCCHHHHCCCCCEEEEEC
MEKRHGEMKPVIQKALVKLDGAPFKAFAANRDEWALTTSYVYPGPIQYFGPTEVCDQPTK
CHHHCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEEEECCCCCCCCCCHHHHCCCCC
TLQLEQA
CEEECCC
>Mature Secondary Structure 
TKSALQIARAAYQPKVPAALKGAVKAVDGEYTQSVADQEEIKKLFPNTYGMPIVTFEKS
CHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHCCCCCCCEEEEEECC
NEEKAMPVMNVGVILSGGQAPGGHNVIAGLFDGIKAHNADSRLYGFILGPGGLIDHKYME
CCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHH
LTADIIDEYRNTGGFDMIGSGRTKLETKEQFDKGLQILKELDIKALVIIGGDDSNTNACV
HHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEE
LAEYYKAIGAGVQVIGCPKTIDGDLKNAQIETSFGFDTACKVYSEVIGNIQRDCNSAQKY
HHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHFIKLMGRSASHIALECALQTQPNVCIISEEVEEKNMSLDDIVTYVAGIVAKRAAEGNN
HHHHHHHCCCCCEEEEEEEECCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
FGTVLIPEGLIEFVPAMKRLIAELNDFLAKHDAEFKMIKKSEQRAYIISKLTKENSDLYA
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCEEE
SLPEGVARQLSLDRDPHGNVQVSLIETEKLLSEMVGKKLAEWKAEGKYVGKFAAQHHFFG
ECHHHHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
YEGRCAAPSNYDADYCYSLGYTASCLIAAGKTGYMSSVRNTTAPADQWIAGGIPVTMMMN
CCCCCCCCCCCCCHHHEECCCCEEEEEECCCCHHHHHHCCCCCCCHHHHCCCCCEEEEEC
MEKRHGEMKPVIQKALVKLDGAPFKAFAANRDEWALTTSYVYPGPIQYFGPTEVCDQPTK
CHHHCCCHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEEEECCCCCCCCCCHHHHCCCCC
TLQLEQA
CEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]