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The map label for this gene is oprM [H]

Identifier: 150008126

GI number: 150008126

Start: 1740846

End: 1742198

Strand: Reverse

Name: oprM [H]

Synonym: BDI_1491

Alternate gene names: 150008126

Gene position: 1742198-1740846 (Counterclockwise)

Preceding gene: 150008127

Following gene: 150008125

Centisome position: 36.21

GC content: 52.85

Gene sequence:

>1353_bases
ATGATGACAAAGAATTTCTATATAGCCGCTTTCGTCGTTTCTATGCTGGCGTTCTCTTCCTGCAAGATCGGAAAGCGGTA
CGTACGTCCGGAGTTGAACCTGCCGGACCGGATCGAGGCTTATACGGATACGACCGATACGGCATCCGTGGAGAATATCC
CATGGGAGAGCCTTTACGCCGATGAGGCCTTGAAGGATCTGATCAGAAAGGCGCTGGATAATAATAAGGATATGAAGATC
GCCGCCAAGCGGATCTCTTTCGCGAGTATGCTGCCGGAGGTGGGAGCGAATGTCTATGCGCAAAAGGAACGCTTGAATTA
TGGAGGGGATAACCCAAAGCCCGATCCAGAATTTGGGGCGAAGCTGACTTTGTCTTGGGAATTAGATTTATGGGGCAACC
TTCGCTGGGCGAATGAGGCAGCCATCGCTTCCTATATGGAGACCGTGGAAGGACAACGTGCCTTGCGATTGACCATCATC
GCCGAGGTTGCCGCCAATTATTATCAACTTTGCGCCTTGGACCAAGAGTTGTCGATCGTACAGCAAACTTTGGACGCACG
CCGTGAGGGTGTTCGCTTGGCGAAGCTTCGCTATGAGGGCGGACTTACTTCCGAGACGGCTTACAACCAAGCCCAAGTGG
AGTTGGCCCGTACCGAGACCTTGATCCCCTCGCTGGAGCGTCAGATCAAGATCAAGGAAAGCGACCTCAGCATGTTGTTG
GGCGAGTATACGGATGATATCCCCCGTGGTCTTTCCTTGAGCGACCAGCACTTGCCGGATGCCTTACCGATCGGCTTACC
GTCTTCTTTGCTGGAGCGCCGACCGGACATGCGTCAAGCGGAGTTTAAGCTTCGGGCGGCGAACGCTCAGGTAGGTGTGG
CGCAAACGGACTTATTCCCGAAGATCCGTTTGACAAGTAATTACGGTTTCGAGAGCGATGAGTTAAGCAGCATCTTGAAG
AGTCCGGCTGGTTTTATCGCCGGAAGCCTGTTGCAGCCGATCTTCGCCATGGGGAAGAACAAGGCGAAAGTGAAAGCGGC
GAAGGCTCGTTACGAGCAAGAGGTCTATAGTTATGAAAAGAGCGTATTAGGCGCTTTCAAGGAGGTGAACAACGCTATCG
TGACCGTCCGCAAGGTGAAGGAGATCCGTGCTTCCCGCATGAATCTGGAAGCCTCCGCCAGTAAATATCTGGAACTCGCC
CAACTGCAATACATCAACGGTGTCATCAGCTACATGGACGTATTGGATGCCCAGCGTGGTTTGCTGGACGCCCAGATCGG
TTTGAACAATGCCGTGCTGGATGAGTTGCTGTCGGTGGTATACCTGTATAAGGCTTTAGGGGGAGGGTTTTGA

Upstream 100 bases:

>100_bases
TTTTGATCTATAAGATAAAGGGAAAGATGCGTATGGAACGTATCGCTCAAAAGAAAGAAGTGGTAACCTCTATTTTAAAG
AGACACAAAAACGGAAAATT

Downstream 100 bases:

>100_bases
TAGCGCACCCTCCTCATAGAGGAAACTATCTCAATGGAATATGCCCCGTAAATCAATGGAATCTACTTCGTGTTCCATTG
ATTTACGGGGCATATTCCAT

Product: outer membrane protein oprM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MMTKNFYIAAFVVSMLAFSSCKIGKRYVRPELNLPDRIEAYTDTTDTASVENIPWESLYADEALKDLIRKALDNNKDMKI
AAKRISFASMLPEVGANVYAQKERLNYGGDNPKPDPEFGAKLTLSWELDLWGNLRWANEAAIASYMETVEGQRALRLTII
AEVAANYYQLCALDQELSIVQQTLDARREGVRLAKLRYEGGLTSETAYNQAQVELARTETLIPSLERQIKIKESDLSMLL
GEYTDDIPRGLSLSDQHLPDALPIGLPSSLLERRPDMRQAEFKLRAANAQVGVAQTDLFPKIRLTSNYGFESDELSSILK
SPAGFIAGSLLQPIFAMGKNKAKVKAAKARYEQEVYSYEKSVLGAFKEVNNAIVTVRKVKEIRASRMNLEASASKYLELA
QLQYINGVISYMDVLDAQRGLLDAQIGLNNAVLDELLSVVYLYKALGGGF

Sequences:

>Translated_450_residues
MMTKNFYIAAFVVSMLAFSSCKIGKRYVRPELNLPDRIEAYTDTTDTASVENIPWESLYADEALKDLIRKALDNNKDMKI
AAKRISFASMLPEVGANVYAQKERLNYGGDNPKPDPEFGAKLTLSWELDLWGNLRWANEAAIASYMETVEGQRALRLTII
AEVAANYYQLCALDQELSIVQQTLDARREGVRLAKLRYEGGLTSETAYNQAQVELARTETLIPSLERQIKIKESDLSMLL
GEYTDDIPRGLSLSDQHLPDALPIGLPSSLLERRPDMRQAEFKLRAANAQVGVAQTDLFPKIRLTSNYGFESDELSSILK
SPAGFIAGSLLQPIFAMGKNKAKVKAAKARYEQEVYSYEKSVLGAFKEVNNAIVTVRKVKEIRASRMNLEASASKYLELA
QLQYINGVISYMDVLDAQRGLLDAQIGLNNAVLDELLSVVYLYKALGGGF
>Mature_450_residues
MMTKNFYIAAFVVSMLAFSSCKIGKRYVRPELNLPDRIEAYTDTTDTASVENIPWESLYADEALKDLIRKALDNNKDMKI
AAKRISFASMLPEVGANVYAQKERLNYGGDNPKPDPEFGAKLTLSWELDLWGNLRWANEAAIASYMETVEGQRALRLTII
AEVAANYYQLCALDQELSIVQQTLDARREGVRLAKLRYEGGLTSETAYNQAQVELARTETLIPSLERQIKIKESDLSMLL
GEYTDDIPRGLSLSDQHLPDALPIGLPSSLLERRPDMRQAEFKLRAANAQVGVAQTDLFPKIRLTSNYGFESDELSSILK
SPAGFIAGSLLQPIFAMGKNKAKVKAAKARYEQEVYSYEKSVLGAFKEVNNAIVTVRKVKEIRASRMNLEASASKYLELA
QLQYINGVISYMDVLDAQRGLLDAQIGLNNAVLDELLSVVYLYKALGGGF

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=455, Percent_Identity=30.1098901098901, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI2367348, Length=331, Percent_Identity=25.3776435045317, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50156; Mature: 50156

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTKNFYIAAFVVSMLAFSSCKIGKRYVRPELNLPDRIEAYTDTTDTASVENIPWESLYA
CCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHH
DEALKDLIRKALDNNKDMKIAAKRISFASMLPEVGANVYAQKERLNYGGDNPKPDPEFGA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCC
KLTLSWELDLWGNLRWANEAAIASYMETVEGQRALRLTIIAEVAANYYQLCALDQELSIV
EEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QQTLDARREGVRLAKLRYEGGLTSETAYNQAQVELARTETLIPSLERQIKIKESDLSMLL
HHHHHHHHCCCEEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHEEECHHHHHHHH
GEYTDDIPRGLSLSDQHLPDALPIGLPSSLLERRPDMRQAEFKLRAANAQVGVAQTDLFP
HHHHHHCCCCCCCCHHCCCCCCCCCCCHHHHHCCCCCHHHHHHEEECCCCCCEEHHCCCC
KIRLTSNYGFESDELSSILKSPAGFIAGSLLQPIFAMGKNKAKVKAAKARYEQEVYSYEK
EEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SVLGAFKEVNNAIVTVRKVKEIRASRMNLEASASKYLELAQLQYINGVISYMDVLDAQRG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LLDAQIGLNNAVLDELLSVVYLYKALGGGF
CCHHHCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMTKNFYIAAFVVSMLAFSSCKIGKRYVRPELNLPDRIEAYTDTTDTASVENIPWESLYA
CCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHH
DEALKDLIRKALDNNKDMKIAAKRISFASMLPEVGANVYAQKERLNYGGDNPKPDPEFGA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCC
KLTLSWELDLWGNLRWANEAAIASYMETVEGQRALRLTIIAEVAANYYQLCALDQELSIV
EEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QQTLDARREGVRLAKLRYEGGLTSETAYNQAQVELARTETLIPSLERQIKIKESDLSMLL
HHHHHHHHCCCEEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHEEECHHHHHHHH
GEYTDDIPRGLSLSDQHLPDALPIGLPSSLLERRPDMRQAEFKLRAANAQVGVAQTDLFP
HHHHHHCCCCCCCCHHCCCCCCCCCCCHHHHHCCCCCHHHHHHEEECCCCCCEEHHCCCC
KIRLTSNYGFESDELSSILKSPAGFIAGSLLQPIFAMGKNKAKVKAAKARYEQEVYSYEK
EEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SVLGAFKEVNNAIVTVRKVKEIRASRMNLEASASKYLELAQLQYINGVISYMDVLDAQRG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LLDAQIGLNNAVLDELLSVVYLYKALGGGF
CCHHHCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]