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The map label for this gene is dnaN [H]

Identifier: 150005411

GI number: 150005411

Start: 3660459

End: 3661583

Strand: Reverse

Name: dnaN [H]

Synonym: BVU_2894

Alternate gene names: 150005411

Gene position: 3661583-3660459 (Counterclockwise)

Preceding gene: 150005413

Following gene: 150005410

Centisome position: 70.92

GC content: 44.8

Gene sequence:

>1125_bases
ATGAAGTTCATCGTTTCAAGTACTGGATTATTCAGTCATTTACAAGCTATTAGCCGAGTAATCAACTCTAAAAATTCGTT
ACCTATTCTTGATTGTTTCTTAATGGAACTGACTGACGGAACGTTGTCGCTCACTGCCTCTGACAGCGAAACGACCCTCT
CCACTTCTTTGGAAGTAAATGAAAGTGACGGTGACGGACGCTTTGCCGTTTCTTCAAAAACCATACTCGAAGCCCTGAAA
GAAATACCCGAACAACCACTGACTTTCCTGGTAAATACAGAGAATCTGGAAATCACCGTACAATATCAGAATGGTAAATA
CAGCCTGATGGGACAAAATGCCGACGAATATCCGCAGGCTCCCGCATTAGGCGCAAATGCGGTACACGTAACAATGGGCG
CTCCGGTGATGCTGGCGGGTATCAACCGTTCCTTGTTCGCTACTGCCGATGACGAGTTACGTCCGGTAATGAATGGTATT
TATTTTGATATCACTACCGAAGACATCACTTTCGTAGCCTCAGACGGGCACAAATTAGTACGCAATAAAACATTTGTGGC
ACACGGCGATGAAAAAGCCGCTTTCATTCTGCCCAAGAAACCGGCAACCCTGCTGAAGAACCTGTTGCCGAAAGAACAAG
GAGATGTGCAAATTGATTTTGACGACCGTAACGCTACGTTTACATTAGAAAACTACAGCATGATATGCCGTCTTATCGAA
GGACGCTATCCCAATTATAATTCAGTAATTCCGCAGGATAATCCGCACAAGGCCACCATTGACCGCATGATGCTGATCAG
TGCGCTCCGCCGTGTATCTGTATTCTCATCACAAGCAAGCAGTTTGATAAAACTCCGCCTCAGTGAAAATCAGATCCAGA
TCTCCGCACAAGACATAGACTTCTCCACCTCAGCAGAAGAAACACTGACTTGCCAATATACAGGAAGCCCCATGAGCATC
GGCTTTAAATCAACATTCCTGATCGATATTTTAAACAATATCTCGGCACAGGAAATACTTTTCGAACTGGCTGATCCTTC
ACGTGCGGGTGTTATTGTTCCTGTAGAACAGGAAGAAAAAGAAGACCTGCTGATGCTGCTGATGCCTATGATGCTGAACG
ACTAA

Upstream 100 bases:

>100_bases
ATTTTTTCGCCATTTTTTGCGCTTGAACCATAATCTTTTCGTAACTTTGGCAGTTAAATGAATATAGAGATATAGAATAT
AAATATAATAATATAAAACT

Downstream 100 bases:

>100_bases
AAACAACACGCCACCATGTGGCGGAAAAACAATATATAATACAAGCTGAAAATGAAATTGAACTTGAAGAATCCTATTGT
TTTTTTCGATTTGGAAACAA

Product: DNA polymerase III, beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKFIVSSTGLFSHLQAISRVINSKNSLPILDCFLMELTDGTLSLTASDSETTLSTSLEVNESDGDGRFAVSSKTILEALK
EIPEQPLTFLVNTENLEITVQYQNGKYSLMGQNADEYPQAPALGANAVHVTMGAPVMLAGINRSLFATADDELRPVMNGI
YFDITTEDITFVASDGHKLVRNKTFVAHGDEKAAFILPKKPATLLKNLLPKEQGDVQIDFDDRNATFTLENYSMICRLIE
GRYPNYNSVIPQDNPHKATIDRMMLISALRRVSVFSSQASSLIKLRLSENQIQISAQDIDFSTSAEETLTCQYTGSPMSI
GFKSTFLIDILNNISAQEILFELADPSRAGVIVPVEQEEKEDLLMLLMPMMLND

Sequences:

>Translated_374_residues
MKFIVSSTGLFSHLQAISRVINSKNSLPILDCFLMELTDGTLSLTASDSETTLSTSLEVNESDGDGRFAVSSKTILEALK
EIPEQPLTFLVNTENLEITVQYQNGKYSLMGQNADEYPQAPALGANAVHVTMGAPVMLAGINRSLFATADDELRPVMNGI
YFDITTEDITFVASDGHKLVRNKTFVAHGDEKAAFILPKKPATLLKNLLPKEQGDVQIDFDDRNATFTLENYSMICRLIE
GRYPNYNSVIPQDNPHKATIDRMMLISALRRVSVFSSQASSLIKLRLSENQIQISAQDIDFSTSAEETLTCQYTGSPMSI
GFKSTFLIDILNNISAQEILFELADPSRAGVIVPVEQEEKEDLLMLLMPMMLND
>Mature_374_residues
MKFIVSSTGLFSHLQAISRVINSKNSLPILDCFLMELTDGTLSLTASDSETTLSTSLEVNESDGDGRFAVSSKTILEALK
EIPEQPLTFLVNTENLEITVQYQNGKYSLMGQNADEYPQAPALGANAVHVTMGAPVMLAGINRSLFATADDELRPVMNGI
YFDITTEDITFVASDGHKLVRNKTFVAHGDEKAAFILPKKPATLLKNLLPKEQGDVQIDFDDRNATFTLENYSMICRLIE
GRYPNYNSVIPQDNPHKATIDRMMLISALRRVSVFSSQASSLIKLRLSENQIQISAQDIDFSTSAEETLTCQYTGSPMSI
GFKSTFLIDILNNISAQEILFELADPSRAGVIVPVEQEEKEDLLMLLMPMMLND

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=26.6666666666667, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41322; Mature: 41322

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVSSTGLFSHLQAISRVINSKNSLPILDCFLMELTDGTLSLTASDSETTLSTSLEVN
CEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHEEEHCCCCEEEEEECCCCCEEEEEEEEE
ESDGDGRFAVSSKTILEALKEIPEQPLTFLVNTENLEITVQYQNGKYSLMGQNADEYPQA
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCEEEEECCCCCCCCCC
PALGANAVHVTMGAPVMLAGINRSLFATADDELRPVMNGIYFDITTEDITFVASDGHKLV
CCCCCCEEEEEECCCEEEEECCCEEEECCCHHHHHHHCCEEEEEECCCEEEEECCCCEEH
RNKTFVAHGDEKAAFILPKKPATLLKNLLPKEQGDVQIDFDDRNATFTLENYSMICRLIE
HCCEEEEECCCCEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHH
GRYPNYNSVIPQDNPHKATIDRMMLISALRRVSVFSSQASSLIKLRLSENQIQISAQDID
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEEEECCC
FSTSAEETLTCQYTGSPMSIGFKSTFLIDILNNISAQEILFELADPSRAGVIVPVEQEEK
CCCCCCCEEEEEECCCCEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCCCH
EDLLMLLMPMMLND
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKFIVSSTGLFSHLQAISRVINSKNSLPILDCFLMELTDGTLSLTASDSETTLSTSLEVN
CEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHEEEHCCCCEEEEEECCCCCEEEEEEEEE
ESDGDGRFAVSSKTILEALKEIPEQPLTFLVNTENLEITVQYQNGKYSLMGQNADEYPQA
CCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCEEEEECCCCCCCCCC
PALGANAVHVTMGAPVMLAGINRSLFATADDELRPVMNGIYFDITTEDITFVASDGHKLV
CCCCCCEEEEEECCCEEEEECCCEEEECCCHHHHHHHCCEEEEEECCCEEEEECCCCEEH
RNKTFVAHGDEKAAFILPKKPATLLKNLLPKEQGDVQIDFDDRNATFTLENYSMICRLIE
HCCEEEEECCCCEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHH
GRYPNYNSVIPQDNPHKATIDRMMLISALRRVSVFSSQASSLIKLRLSENQIQISAQDID
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEEEECCC
FSTSAEETLTCQYTGSPMSIGFKSTFLIDILNNISAQEILFELADPSRAGVIVPVEQEEK
CCCCCCCEEEEEECCCCEEECCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCCCH
EDLLMLLMPMMLND
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]