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The map label for this gene is gmd [H]

Identifier: 150005182

GI number: 150005182

Start: 3370732

End: 3371811

Strand: Reverse

Name: gmd [H]

Synonym: BVU_2652

Alternate gene names: 150005182

Gene position: 3371811-3370732 (Counterclockwise)

Preceding gene: 150005183

Following gene: 150005181

Centisome position: 65.3

GC content: 47.69

Gene sequence:

>1080_bases
ATGAGTAAAAAAGTAGCATTGATTTCAGGTATCACAGGACAGGACGGTTCGTTCCTGGCCGAGTTCCTGATAGAAAAAGG
ATACGAGGTACATGGTATCCTGAGACGTTCTTCTTCATTTAACACTGCACGTATAGAACATCTGTATCTGGACGAATGGG
TACGGGATATGAAGCAGAACCGACTGGTGAACCTGCATTGGGGGGATATGACGGACAGTAGCTCACTGATACGTATCATA
CAGTCCGTACAACCTGACGAGATTTATAATCTGGCCGCACAAAGCCATGTAAAAGTAAGCTTCGACGTGCCCGAATACAC
AGCGGAAGCGGATGCGGTAGGTACGCTGCGCATGCTGGAGGCGGTACGTATATTGGGGCTGGAAAAGAAAACCAAGATTT
ATCAGGCATCTACTTCGGAACTTTTCGGACTGGTGCAGGAAGTTCCTCAGAAAGAGACTACTCCTTTCTATCCCCGTTCT
CCATACGGAGTAGCCAAGCAATATGGTTTCTGGATAACCAAGAATTACCGCGAAAGCTATGGCATGTTTGCCGTAAACGG
TATTTTGTTCAATCACGAAAGTGAACGCCGGGGCGAAACTTTTGTTACCCGGAAAATTACCCTGGCGGCGGCACGCATCG
CACAAGGATTCCAAGATAAACTGTACTTGGGGAACCTGAACTCCCTGCGCGACTGGGGATATGCCAAAGACTATGTGGAA
TGTATGTGGCTGATTCTGCAACACGATACGCCCGAAGACTTTGTTATAGCCACCGGAGAATATCACACTGTACGCGAATT
TGCTACCCTTGCTTTCAAGGAAGCGGGCATCGACTTGCGCTGGGAAGGTGAAGGTGTGAACGAGAAAGGTATTGACACAG
CTACAGGCAAGGTGCTGGTAGAGGTGGATCCCAAATATTTCCGCCCGGCAGAGGTGGAACAATTACTGGGAGATCCTACC
AAAGCCAAAACGTTACTGGGATGGAATCCCCGCAAGACTTCATTCGAGGAACTGGTGAAAATCATGGTGAAACACGATAT
GAAATTCGTGAAGAAACTTCATCTCAAAGCAAGCTGTTAA

Upstream 100 bases:

>100_bases
GCAAATAAAAAAGAATAAGAATTATTTATACCGGTCCAAATGCAAGTATGCATGAAGACAGGTCATGGAATAAAACATTT
TTTCTAGAATAATACAAAAT

Downstream 100 bases:

>100_bases
ATCATTAGTCAAGAATTTCAATGGAGAAATCAGCCAAAATTTATATAGCAGGGCACCACGGACTCGTGGGCTCCGCTATT
TGGCGTAACCTGCAAGAAAA

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTARIEHLYLDEWVRDMKQNRLVNLHWGDMTDSSSLIRII
QSVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLEAVRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRS
PYGVAKQYGFWITKNYRESYGMFAVNGILFNHESERRGETFVTRKITLAAARIAQGFQDKLYLGNLNSLRDWGYAKDYVE
CMWLILQHDTPEDFVIATGEYHTVREFATLAFKEAGIDLRWEGEGVNEKGIDTATGKVLVEVDPKYFRPAEVEQLLGDPT
KAKTLLGWNPRKTSFEELVKIMVKHDMKFVKKLHLKASC

Sequences:

>Translated_359_residues
MSKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTARIEHLYLDEWVRDMKQNRLVNLHWGDMTDSSSLIRII
QSVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLEAVRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRS
PYGVAKQYGFWITKNYRESYGMFAVNGILFNHESERRGETFVTRKITLAAARIAQGFQDKLYLGNLNSLRDWGYAKDYVE
CMWLILQHDTPEDFVIATGEYHTVREFATLAFKEAGIDLRWEGEGVNEKGIDTATGKVLVEVDPKYFRPAEVEQLLGDPT
KAKTLLGWNPRKTSFEELVKIMVKHDMKFVKKLHLKASC
>Mature_358_residues
SKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTARIEHLYLDEWVRDMKQNRLVNLHWGDMTDSSSLIRIIQ
SVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLEAVRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRSP
YGVAKQYGFWITKNYRESYGMFAVNGILFNHESERRGETFVTRKITLAAARIAQGFQDKLYLGNLNSLRDWGYAKDYVEC
MWLILQHDTPEDFVIATGEYHTVREFATLAFKEAGIDLRWEGEGVNEKGIDTATGKVLVEVDPKYFRPAEVEQLLGDPTK
AKTLLGWNPRKTSFEELVKIMVKHDMKFVKKLHLKASC

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=349, Percent_Identity=62.1776504297994, Blast_Score=442, Evalue=1e-124,
Organism=Escherichia coli, GI1788366, Length=365, Percent_Identity=63.8356164383562, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI48994969, Length=185, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788353, Length=256, Percent_Identity=25.390625, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=349, Percent_Identity=64.1833810888252, Blast_Score=460, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17539424, Length=350, Percent_Identity=63.1428571428571, Blast_Score=459, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI133901790, Length=350, Percent_Identity=63.1428571428571, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI133901788, Length=350, Percent_Identity=63.1428571428571, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17539422, Length=350, Percent_Identity=63.1428571428571, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI133901786, Length=350, Percent_Identity=63.1428571428571, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24158427, Length=349, Percent_Identity=62.1776504297994, Blast_Score=452, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 40981; Mature: 40850

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS01172 RIBOSOMAL_L44E ; PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTARIEHLYLDEWVRDMKQN
CCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
RLVNLHWGDMTDSSSLIRIIQSVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLE
CEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHH
AVRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRSPYGVAKQYGFWITKNYRESY
HHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEECCHHHHC
GMFAVNGILFNHESERRGETFVTRKITLAAARIAQGFQDKLYLGNLNSLRDWGYAKDYVE
CEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCCHHHHHH
CMWLILQHDTPEDFVIATGEYHTVREFATLAFKEAGIDLRWEGEGVNEKGIDTATGKVLV
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEE
EVDPKYFRPAEVEQLLGDPTKAKTLLGWNPRKTSFEELVKIMVKHDMKFVKKLHLKASC
EECCCCCCCHHHHHHHCCCCHHCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKKVALISGITGQDGSFLAEFLIEKGYEVHGILRRSSSFNTARIEHLYLDEWVRDMKQN
CCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHC
RLVNLHWGDMTDSSSLIRIIQSVQPDEIYNLAAQSHVKVSFDVPEYTAEADAVGTLRMLE
CEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHH
AVRILGLEKKTKIYQASTSELFGLVQEVPQKETTPFYPRSPYGVAKQYGFWITKNYRESY
HHHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCEEEECCHHHHC
GMFAVNGILFNHESERRGETFVTRKITLAAARIAQGFQDKLYLGNLNSLRDWGYAKDYVE
CEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHCCCHHHHHH
CMWLILQHDTPEDFVIATGEYHTVREFATLAFKEAGIDLRWEGEGVNEKGIDTATGKVLV
HHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEE
EVDPKYFRPAEVEQLLGDPTKAKTLLGWNPRKTSFEELVKIMVKHDMKFVKKLHLKASC
EECCCCCCCHHHHHHHCCCCHHCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]