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The map label for this gene is rsmD [H]

Identifier: 148827957

GI number: 148827957

Start: 1361011

End: 1361592

Strand: Reverse

Name: rsmD [H]

Synonym: CGSHiGG_07330

Alternate gene names: 148827957

Gene position: 1361592-1361011 (Counterclockwise)

Preceding gene: 148827961

Following gene: 148827956

Centisome position: 72.15

GC content: 36.77

Gene sequence:

>582_bases
ATGAAAAAAATACAAACGCCAAATGCAAAGGGCGAGGTTCGCATTATTGCAGGTCTATGGCGAGGGCGAAAACTTCCCGT
ATTAAATTCTGAAGGCTTACGCCCAACTGGCGATAGAGTGAAAGAAACGCTTTTTAATTGGTTAATGCCTTATATCCACC
AATCTGAATGTTTAGATGGCTTTGCGGGGAGTGGTTCGTTAGGTTTTGAAGCACTTTCTCGCCAAGCGAAAAAAGTGACT
TTTTTAGAATTGGATAAAACCGTTGCCAATCAATTAAAAAAGAATTTACAAACACTCAAATGCTCATCAGAACAGGCTGA
AGTAATTAATCAAAGTAGTTTGGATTTTCTAAAACAGCCACAAAATCAACCGCACTTTGATGTGGTGTTTTTAGATCCAC
CTTTTCATTTTAATTTGGCAGAACAAGCAATTAGTTTACTTTGTGAAAATAATTGGCTAAAACCCAATGCGTTAATTTAC
GTAGAAACAGAGAAAGATAAACCGCTTATTACTCCTGAAAACTGGACGTTATTGAAAGAAAAAACGACGGGAATGGTGAG
TTATCGTTTATATCAGAATTAG

Upstream 100 bases:

>100_bases
CCATAAAATGCTCTCTTAACTGTAATCCGATAAAAATGTCGTTCATTCTAGCAAAAAATCGTCTAAACTAAGCACAATTT
TCAATAAAAATCGAATCTTT

Downstream 100 bases:

>100_bases
AGAAAAAATTATGTTAGATATTGTTTTATATGAACCAGAAATCCCACAAAATACTGGTAACATTATTCGACTTTGTGCCA
ACACAGGATTTCGCTTGCAC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDGFAGSGSLGFEALSRQAKKVT
FLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQPQNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIY
VETEKDKPLITPENWTLLKEKTTGMVSYRLYQN

Sequences:

>Translated_193_residues
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDGFAGSGSLGFEALSRQAKKVT
FLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQPQNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIY
VETEKDKPLITPENWTLLKEKTTGMVSYRLYQN
>Mature_193_residues
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDGFAGSGSLGFEALSRQAKKVT
FLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQPQNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIY
VETEKDKPLITPENWTLLKEKTTGMVSYRLYQN

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=192, Percent_Identity=54.6875, Blast_Score=205, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22020; Mature: 22020

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDG
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
FAGSGSLGFEALSRQAKKVTFLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQP
CCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
QNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIYVETEKDKPLITPENWTLLKE
CCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEECCCCCEEEEE
KTTGMVSYRLYQN
CCCCEEEEEEECC
>Mature Secondary Structure
MKKIQTPNAKGEVRIIAGLWRGRKLPVLNSEGLRPTGDRVKETLFNWLMPYIHQSECLDG
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
FAGSGSLGFEALSRQAKKVTFLELDKTVANQLKKNLQTLKCSSEQAEVINQSSLDFLKQP
CCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
QNQPHFDVVFLDPPFHFNLAEQAISLLCENNWLKPNALIYVETEKDKPLITPENWTLLKE
CCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEECCCCCEEEEE
KTTGMVSYRLYQN
CCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]