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The map label for this gene is ansB

Identifier: 148827936

GI number: 148827936

Start: 1335314

End: 1336363

Strand: Direct

Name: ansB

Synonym: CGSHiGG_07215

Alternate gene names: 148827936

Gene position: 1335314-1336363 (Clockwise)

Preceding gene: 148827933

Following gene: 148827937

Centisome position: 70.76

GC content: 39.24

Gene sequence:

>1050_bases
ATGAAATTAACTAAATTAGCCTTATGTACTTTATTTGGTCTAGGTGTTTCAATAGCAAATGCAGCTGACTTACCAAATAT
TACAATCTTGGCAACGGGTGGTACCATTGCAGGAAGTGGGCAAAGTTCGGTAAATTCTGCGTATAAAGCTGGACAATTAA
GTATTGATACTTTAATTGAAGCTGTACCAGAAATGAAAAATATTGCCAACATTAAAGGTGAGCAAATTGTAAAAATAGGT
TCACAAGACATGAATGACGAAGTCTGGCTAAAACTGGCAAAAGCCATCAATGCTCAATGTAAAAGTACTGATGGATTTGT
CATTACCCATGGTACAGATACCATGGAAGAGACGGCTTATTTCTTAGATTTAACCGTAAAATGTGAAAAACCGGTTGTTC
TCGTTGGGGCAATGCGTCCTGCAACAGAAAAAAGTGCTGATGGCCCATTAAATCTTTACAATGCTGTCGTTGTCGCAGCA
GACAAAAAATCAAGTGGTCGTGGTGTTTTAGTCGCAATGAATAATGAAGTACTAGGTGCTCGCGATGTAACAAAAACCAG
TACGACCGCAGTGCAAACGTTCCATTCACCAAATTATGGTTCTCTAGGCTATATTCATAACAGCAAAGTGGACTATGAAC
GTTCCCCAGAAAGCAAACATACCATCAACACTCCGTTTAATGTAGAAAAGTTAGATAGCCTACCCAAAGTGGGGATTATT
TATGCTTATTCAAATGCACCTGTCGAACCATTAAACGCATTACTCAATGCTGGCTATCAAGGGATTGTATCTGCAGGAGT
TGGCAATGGAAATGTTAATGCCGCACACTTAGATCGCTTAGAAAAAGCCGCAAAAGATAGCGTCGTTGTAGTCCGTTCTT
CTCGTGTACCAACGGGTTATACAACTCGTGACGCTGAAGTTGATGATAGTAAATATGGCTTTGTAGCATCAGGTACTTTA
AATCCACAGAAAGCCCGCGTGCTCTTGCAATTAGCTTTAACTCAAACTAAAGATCCAAAAGTAATTCAACAATATTTCGA
AGACTTCTAA

Upstream 100 bases:

>100_bases
TATGTGATCGAGATCATAAATTTGTTTTTCCATTGTTTATAAATAACTTAATTTAGTTAGACTACTTTGGAGAAATTTTT
ATTCATCTTAGGAGAAATCT

Downstream 100 bases:

>100_bases
GATCAATTGTAATAAAAAAAAGTGCGGTTATAAATAACCGCACTTTTGACCACAATTTATTGATCCAAGTTATGGAATCT
CTACCTATTTGTTGCTATAT

Product: L-asparaginase II

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKLTKLALCTLFGLGVSIANAADLPNITILATGGTIAGSGQSSVNSAYKAGQLSIDTLIEAVPEMKNIANIKGEQIVKIG
SQDMNDEVWLKLAKAINAQCKSTDGFVITHGTDTMEETAYFLDLTVKCEKPVVLVGAMRPATEKSADGPLNLYNAVVVAA
DKKSSGRGVLVAMNNEVLGARDVTKTSTTAVQTFHSPNYGSLGYIHNSKVDYERSPESKHTINTPFNVEKLDSLPKVGII
YAYSNAPVEPLNALLNAGYQGIVSAGVGNGNVNAAHLDRLEKAAKDSVVVVRSSRVPTGYTTRDAEVDDSKYGFVASGTL
NPQKARVLLQLALTQTKDPKVIQQYFEDF

Sequences:

>Translated_349_residues
MKLTKLALCTLFGLGVSIANAADLPNITILATGGTIAGSGQSSVNSAYKAGQLSIDTLIEAVPEMKNIANIKGEQIVKIG
SQDMNDEVWLKLAKAINAQCKSTDGFVITHGTDTMEETAYFLDLTVKCEKPVVLVGAMRPATEKSADGPLNLYNAVVVAA
DKKSSGRGVLVAMNNEVLGARDVTKTSTTAVQTFHSPNYGSLGYIHNSKVDYERSPESKHTINTPFNVEKLDSLPKVGII
YAYSNAPVEPLNALLNAGYQGIVSAGVGNGNVNAAHLDRLEKAAKDSVVVVRSSRVPTGYTTRDAEVDDSKYGFVASGTL
NPQKARVLLQLALTQTKDPKVIQQYFEDF
>Mature_349_residues
MKLTKLALCTLFGLGVSIANAADLPNITILATGGTIAGSGQSSVNSAYKAGQLSIDTLIEAVPEMKNIANIKGEQIVKIG
SQDMNDEVWLKLAKAINAQCKSTDGFVITHGTDTMEETAYFLDLTVKCEKPVVLVGAMRPATEKSADGPLNLYNAVVVAA
DKKSSGRGVLVAMNNEVLGARDVTKTSTTAVQTFHSPNYGSLGYIHNSKVDYERSPESKHTINTPFNVEKLDSLPKVGII
YAYSNAPVEPLNALLNAGYQGIVSAGVGNGNVNAAHLDRLEKAAKDSVVVVRSSRVPTGYTTRDAEVDDSKYGFVASGTL
NPQKARVLLQLALTQTKDPKVIQQYFEDF

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family

Homologues:

Organism=Homo sapiens, GI122937331, Length=230, Percent_Identity=27.3913043478261, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789327, Length=345, Percent_Identity=69.2753623188406, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1788065, Length=282, Percent_Identity=29.7872340425532, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=248, Percent_Identity=27.8225806451613, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=248, Percent_Identity=27.8225806451613, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=248, Percent_Identity=27.8225806451613, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193207374, Length=248, Percent_Identity=27.8225806451613, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323189, Length=319, Percent_Identity=39.8119122257053, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6323187, Length=319, Percent_Identity=39.8119122257053, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6323186, Length=319, Percent_Identity=39.8119122257053, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6323184, Length=319, Percent_Identity=39.8119122257053, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320527, Length=332, Percent_Identity=35.5421686746988, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24645530, Length=240, Percent_Identity=29.1666666666667, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639609, Length=225, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASPG2_HAEIN (P43843)

Other databases:

- EMBL:   L42023
- PIR:   A64090
- RefSeq:   NP_438904.1
- ProteinModelPortal:   P43843
- SMR:   P43843
- GeneID:   949675
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0745
- NMPDR:   fig|71421.1.peg.713
- TIGR:   HI_0745
- HOGENOM:   HBG497495
- OMA:   QGVVITH
- ProtClustDB:   PRK11096
- BioCyc:   HINF71421:HI_0745-MONOMER
- BRENDA:   3.5.1.1
- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00520

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: =3.5.1.1

Molecular weight: Translated: 37213; Mature: 37213

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 35-35 BINDING 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKLALCTLFGLGVSIANAADLPNITILATGGTIAGSGQSSVNSAYKAGQLSIDTLIE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEECCCCHHHHHHHHCCCEEHHHHHH
AVPEMKNIANIKGEQIVKIGSQDMNDEVWLKLAKAINAQCKSTDGFVITHGTDTMEETAY
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCEE
FLDLTVKCEKPVVLVGAMRPATEKSADGPLNLYNAVVVAADKKSSGRGVLVAMNNEVLGA
EEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCEECC
RDVTKTSTTAVQTFHSPNYGSLGYIHNSKVDYERSPESKHTINTPFNVEKLDSLPKVGII
CCCCCCCHHEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEECCCCCHHHHHCCCCEEEE
YAYSNAPVEPLNALLNAGYQGIVSAGVGNGNVNAAHLDRLEKAAKDSVVVVRSSRVPTGY
EEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCC
TTRDAEVDDSKYGFVASGTLNPQKARVLLQLALTQTKDPKVIQQYFEDF
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKLTKLALCTLFGLGVSIANAADLPNITILATGGTIAGSGQSSVNSAYKAGQLSIDTLIE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEECCCCHHHHHHHHCCCEEHHHHHH
AVPEMKNIANIKGEQIVKIGSQDMNDEVWLKLAKAINAQCKSTDGFVITHGTDTMEETAY
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCEE
FLDLTVKCEKPVVLVGAMRPATEKSADGPLNLYNAVVVAADKKSSGRGVLVAMNNEVLGA
EEEEEEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCEECC
RDVTKTSTTAVQTFHSPNYGSLGYIHNSKVDYERSPESKHTINTPFNVEKLDSLPKVGII
CCCCCCCHHEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEECCCCCHHHHHCCCCEEEE
YAYSNAPVEPLNALLNAGYQGIVSAGVGNGNVNAAHLDRLEKAAKDSVVVVRSSRVPTGY
EEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCC
TTRDAEVDDSKYGFVASGTLNPQKARVLLQLALTQTKDPKVIQQYFEDF
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023