Click here to switch to the map view.

The map label for this gene is dcuB [H]

Identifier: 148827937

GI number: 148827937

Start: 1336520

End: 1337842

Strand: Direct

Name: dcuB [H]

Synonym: CGSHiGG_07220

Alternate gene names: 148827937

Gene position: 1336520-1337842 (Clockwise)

Preceding gene: 148827936

Following gene: 148827939

Centisome position: 70.82

GC content: 40.74

Gene sequence:

>1323_bases
ATGTCTGCAATGTTTTTACTACAATTTGCCATCGTATTATTGTGTATCTTTGTGGGTGCGCGTGTAGGTGGTATCGGTTT
AGGTGTATTTGGTGGTTTAGGTCTTGCCATTCTTTCTTTTGGCTTTGGCTTGAAACCAGCAGGTTTACCTATTGATGTTA
TGTTTATGATTATGGCTGTAGTGGCAGCCGCGGCAGCAATGCAAGCAGCTGGCGGTTTAGACTACATGATCAAAATCGCA
ACCAAAATCTTACGTCGTAATCCTAAACACATTACGTTTATTGCGCCAGCAGTAACTTGGTTATTCACTCTCTTAGCGGG
TACTGGTCACGTGGCATATTCTGTATTGCCAGTTATCGCTGAAGTGAGCCGTCAAAACGGTATCCGTCCTGAACGCCCAA
TGTCAATGGCGGTAATTGCATCACAATTTGCTATCGTAGCAAGCCCAATCGCTGCAGCGGTGGTGGCTGTGGTTGCTTAC
CTTGAGCCACAAGGCATTCATCTTGGCGATGTTTTAATGGTAACCATTCCTTCAACTATTCTAGGACTTTTCCTTGCTTG
CTTATTTGTTAATAAAATGGGTAAAGAATTAAAAGATGATCCAGAATATCAACGTCGTTTGAATGATCCAAAATATACAG
ATATTTTTGCTTCTACCACTTCAGTCAAAGAAGTAGAAGTGAGCAAAACAGCTAAAATTTCTGTTTCTTTATTCTTATTT
GGTGCTTTACTTGTCGTGTTAATGGGGGCAGCTCCTTCTATTCGACCTGTTTTTGATGGAAAACCAATGGGAATGGCACA
TACCATTGAAATTACTATGCTTTCTATTGGTGCATTGATTATCTTAACTTGTAAACCAGATGGCACAGCGATTACCAAAG
GTTCTGTATTCCATGCAGGTATGCGCGCAGTAATCGCAATCTTCGGTATTGCTTGGTTAGGTGACACTTTAATGCAGGCA
CACATTACTGAAGTAAAAGAAATGGTGAAAGGCTTAGTGGAAACTGCACCATGGGCATTTGCTTTCGCGCTATTTGTACT
TTCAGTATTAGTAAACAGCCAAGGTGCAACAGTAGCGACATTGTTCCCTCTTGGTATCGCATTAGGTATCCCTGCACCTG
TATTAATCGGTGTATTTGTTGCTGTAAATGGTTACTTCTTCATCCCTAACTATGGTCCAATCATTGCATCTCTTGACTTC
GATACCACAGGTACAACCAAAATCGGTAAATACATTTTAAACCATAGCTTTATGCTTCCGGGTTTATTGAGCATGCTTTT
CTGCTTATTAATCGGTTTAGGTCTTTCTCACGTTATTCTTTAA

Upstream 100 bases:

>100_bases
TTATTGATCCAAGTTATGGAATCTCTACCTATTTGTTGCTATATTACACGCCGTTTCAACAAATAAAACACAGTGTTTTT
TTATTAACTTAGGGGGCAGT

Downstream 100 bases:

>100_bases
AATGTGATTCGATAACAAAGAACGGTGCTGAAATAGCACCGTTTTTGTTGTTCTAATATCAACTTAAAATAACTCAAAAA
ATAACCGCACTTTGATATAT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSAMFLLQFAIVLLCIFVGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAVVAAAAAMQAAGGLDYMIKIA
TKILRRNPKHITFIAPAVTWLFTLLAGTGHVAYSVLPVIAEVSRQNGIRPERPMSMAVIASQFAIVASPIAAAVVAVVAY
LEPQGIHLGDVLMVTIPSTILGLFLACLFVNKMGKELKDDPEYQRRLNDPKYTDIFASTTSVKEVEVSKTAKISVSLFLF
GALLVVLMGAAPSIRPVFDGKPMGMAHTIEITMLSIGALIILTCKPDGTAITKGSVFHAGMRAVIAIFGIAWLGDTLMQA
HITEVKEMVKGLVETAPWAFAFALFVLSVLVNSQGATVATLFPLGIALGIPAPVLIGVFVAVNGYFFIPNYGPIIASLDF
DTTGTTKIGKYILNHSFMLPGLLSMLFCLLIGLGLSHVIL

Sequences:

>Translated_440_residues
MSAMFLLQFAIVLLCIFVGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAVVAAAAAMQAAGGLDYMIKIA
TKILRRNPKHITFIAPAVTWLFTLLAGTGHVAYSVLPVIAEVSRQNGIRPERPMSMAVIASQFAIVASPIAAAVVAVVAY
LEPQGIHLGDVLMVTIPSTILGLFLACLFVNKMGKELKDDPEYQRRLNDPKYTDIFASTTSVKEVEVSKTAKISVSLFLF
GALLVVLMGAAPSIRPVFDGKPMGMAHTIEITMLSIGALIILTCKPDGTAITKGSVFHAGMRAVIAIFGIAWLGDTLMQA
HITEVKEMVKGLVETAPWAFAFALFVLSVLVNSQGATVATLFPLGIALGIPAPVLIGVFVAVNGYFFIPNYGPIIASLDF
DTTGTTKIGKYILNHSFMLPGLLSMLFCLLIGLGLSHVIL
>Mature_439_residues
SAMFLLQFAIVLLCIFVGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAVVAAAAAMQAAGGLDYMIKIAT
KILRRNPKHITFIAPAVTWLFTLLAGTGHVAYSVLPVIAEVSRQNGIRPERPMSMAVIASQFAIVASPIAAAVVAVVAYL
EPQGIHLGDVLMVTIPSTILGLFLACLFVNKMGKELKDDPEYQRRLNDPKYTDIFASTTSVKEVEVSKTAKISVSLFLFG
ALLVVLMGAAPSIRPVFDGKPMGMAHTIEITMLSIGALIILTCKPDGTAITKGSVFHAGMRAVIAIFGIAWLGDTLMQAH
ITEVKEMVKGLVETAPWAFAFALFVLSVLVNSQGATVATLFPLGIALGIPAPVLIGVFVAVNGYFFIPNYGPIIASLDFD
TTGTTKIGKYILNHSFMLPGLLSMLFCLLIGLGLSHVIL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=439, Percent_Identity=41.9134396355353, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1790565, Length=390, Percent_Identity=41.7948717948718, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 46542; Mature: 46410

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAMFLLQFAIVLLCIFVGARVGGIGLGVFGGLGLAILSFGFGLKPAGLPIDVMFMIMAV
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VAAAAAMQAAGGLDYMIKIATKILRRNPKHITFIAPAVTWLFTLLAGTGHVAYSVLPVIA
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHH
EVSRQNGIRPERPMSMAVIASQFAIVASPIAAAVVAVVAYLEPQGIHLGDVLMVTIPSTI
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
LGLFLACLFVNKMGKELKDDPEYQRRLNDPKYTDIFASTTSVKEVEVSKTAKISVSLFLF
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHEECCCCCHHEECCCHHHHHHHHHHH
GALLVVLMGAAPSIRPVFDGKPMGMAHTIEITMLSIGALIILTCKPDGTAITKGSVFHAG
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEHHEEEECEEEEEEECCCCCEEECCHHHHHH
MRAVIAIFGIAWLGDTLMQAHITEVKEMVKGLVETAPWAFAFALFVLSVLVNSQGATVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEHH
LFPLGIALGIPAPVLIGVFVAVNGYFFIPNYGPIIASLDFDTTGTTKIGKYILNHSFMLP
HHHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHH
GLLSMLFCLLIGLGLSHVIL
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]