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The map label for this gene is ilvD [H]

Identifier: 148827928

GI number: 148827928

Start: 1324715

End: 1326553

Strand: Direct

Name: ilvD [H]

Synonym: CGSHiGG_07165

Alternate gene names: 148827928

Gene position: 1324715-1326553 (Clockwise)

Preceding gene: 148827927

Following gene: 148827931

Centisome position: 70.2

GC content: 43.34

Gene sequence:

>1839_bases
ATGCCCAAACTACGTTCAGCGACCAGCACACAAGGTCGTAATATGGCAGGCGCACGTGCCTTATGGCGTGCAACAGGAAT
GAAAGAAAATGACTTTGGTAAACCGATTATTGCGGTGGTGAACTCTTTCACGCAATTCGTACCAGGGCATGTGCATTTAA
AAGATATGGGACAGCTTGTTGCCGCAGAAATTGAAAAAGCGGGCGGCGTAGCAAAAGAATTCAATACCATTGCGGTGGAT
GATGGTATCGCAATGGGGCACGGTGGAATGCTTTATTCTTTGCCAAGCCGTGATTTAATCGCTGATAGCGTGGAATATAT
GGTCAATGCGCACTGTGCTGATGCGATGGTGTGTATTTCCAACTGTGACAAAATCACTCCAGGAATGTTGATGGCTGCAA
TGCGTTTAAATATTCCAACTATCTTCGTTTCAGGCGGCCCGATGGAAGCAGGTAAAACTAAACTTTCTGATCAATTAATC
CGATTAGATTTAGTAGATGCGATGATTGAGGCAGCGGATCCGAATGTATCAGATGAACGTATTGATGCCATTGAACGCAG
TGCGTGTCCAACTTGTGGTTCATGCTCAGGTATGTTTACCGCAAACTCTATGAATTGCCTTACTGAAGCCTTAGGTTTAT
CTTTACCAGGCAATGGTTCCATGTTAGCTACTCATGCTGACCGCAAAGAATTATTCTTAAAAGCAGGTCGTCAAATTGTC
GAGCTTTGCAAACGCTATTATGAACAAGATGATGCAAGCGTATTGCCTCGTTCAATCGGTACTTTTGATGCCTTTGAAAA
TGCAATGAGCCTCGATATCGCAATGGGTGGTTCAAGTAACACTGTTTTACACTTATTAGCGGCAGCACAAGAAGCAGGCA
TGGATTTTAAAATGGAAGATATTGACCGTTTATCGCGCAAAGTGCCTTGTTTAAGCAAAATTGCACCAAACACCAATAAA
TATCATATGGAAGACGTACACCGTGCAGGCGGTATTATGGGATTGTTGGGTGAATTAGATCGTGCTGGATTAATTCATAA
AAATACACACACCGTGCTTGGAATGAGCATGGGAGAGCAACTAGACCAATACGATATTATTCGCAATCAAGATGAAGAAT
TACATAAATTCTTCCGTGCAGGCCCTGCAGGTATCCGCACAACTCAAGCATTCTCACAAGATTGCCGTTGGGATACCGTT
GATAATGATCGTGTAAACGGTTGTATTCGCAACAAAGAAAATGCAATTTCGCAAGAAGGTGGCTTAGCCGTATTATTCGG
TAATCTCGCTGAAGACGGATGTATCGTTAAAACCGCTGGTGTAGATGAATCTATTTGGAAATTCACTGGCACAGCAATCG
TATTTGAAAGCCAAGAAGATGCGGTTGCAGGTATTTTAGGTGGCAAAGTCAAAGAAGGTCATGTTGTTGTTATCCGTTAC
GAAGGGCCTAAAGGCGGACCAGGTATGCAAGAAATGTTATACCCAACCAGTTACTTAAAATCGATGGGCTTGGGCAAAAA
ATGCGCTTTATTAACGGATGGTCGCTTCTCTGGCGGTACATCAGGCTTGTCTATTGGACATGCTTCACCAGAAGCTGCTT
CTGGCGGTGCAATCGGCTTAGTACGCGATGGCGATATTATCAACATTGATATTCCAAATCGTGCAATTAACTTAGAAATC
AGTAATGATGAACTCGCAACACGCAGAGCCGAACAAGATCAAAAAGGTTGGCAGCCAGCTAACCGTGAACGTGAAGTCTC
TTTCGCTTTAAAAGTATTCGGTCACTTTGCGACCTCAGCGGACAAAGGTGCGGTACGCGATAAAACATTATTAAAATAA

Upstream 100 bases:

>100_bases
TCTGAAGATCTAGAAAATATCTTGCCAGCTGCTATTTTAGAAAACTTTCTACAATATAAGTAATAATATTTACCTTAAAT
TTTACTCGGAGAGATTATTT

Downstream 100 bases:

>100_bases
TTTAACGGTTCCCCTCTTTCATAAGAGGGGATAAAACAAAGGAAAAACTAACCGCACTTTATCATCAAAACAAACACTAT
GAAAAATCTACTCACCAATC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MPKLRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFTQFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVD
DGIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
RLDLVDAMIEAADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRKELFLKAGRQIV
ELCKRYYEQDDASVLPRSIGTFDAFENAMSLDIAMGGSSNTVLHLLAAAQEAGMDFKMEDIDRLSRKVPCLSKIAPNTNK
YHMEDVHRAGGIMGLLGELDRAGLIHKNTHTVLGMSMGEQLDQYDIIRNQDEELHKFFRAGPAGIRTTQAFSQDCRWDTV
DNDRVNGCIRNKENAISQEGGLAVLFGNLAEDGCIVKTAGVDESIWKFTGTAIVFESQEDAVAGILGGKVKEGHVVVIRY
EGPKGGPGMQEMLYPTSYLKSMGLGKKCALLTDGRFSGGTSGLSIGHASPEAASGGAIGLVRDGDIINIDIPNRAINLEI
SNDELATRRAEQDQKGWQPANREREVSFALKVFGHFATSADKGAVRDKTLLK

Sequences:

>Translated_612_residues
MPKLRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFTQFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVD
DGIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
RLDLVDAMIEAADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRKELFLKAGRQIV
ELCKRYYEQDDASVLPRSIGTFDAFENAMSLDIAMGGSSNTVLHLLAAAQEAGMDFKMEDIDRLSRKVPCLSKIAPNTNK
YHMEDVHRAGGIMGLLGELDRAGLIHKNTHTVLGMSMGEQLDQYDIIRNQDEELHKFFRAGPAGIRTTQAFSQDCRWDTV
DNDRVNGCIRNKENAISQEGGLAVLFGNLAEDGCIVKTAGVDESIWKFTGTAIVFESQEDAVAGILGGKVKEGHVVVIRY
EGPKGGPGMQEMLYPTSYLKSMGLGKKCALLTDGRFSGGTSGLSIGHASPEAASGGAIGLVRDGDIINIDIPNRAINLEI
SNDELATRRAEQDQKGWQPANREREVSFALKVFGHFATSADKGAVRDKTLLK
>Mature_611_residues
PKLRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFTQFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDD
GIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLIR
LDLVDAMIEAADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRKELFLKAGRQIVE
LCKRYYEQDDASVLPRSIGTFDAFENAMSLDIAMGGSSNTVLHLLAAAQEAGMDFKMEDIDRLSRKVPCLSKIAPNTNKY
HMEDVHRAGGIMGLLGELDRAGLIHKNTHTVLGMSMGEQLDQYDIIRNQDEELHKFFRAGPAGIRTTQAFSQDCRWDTVD
NDRVNGCIRNKENAISQEGGLAVLFGNLAEDGCIVKTAGVDESIWKFTGTAIVFESQEDAVAGILGGKVKEGHVVVIRYE
GPKGGPGMQEMLYPTSYLKSMGLGKKCALLTDGRFSGGTSGLSIGHASPEAASGGAIGLVRDGDIINIDIPNRAINLEIS
NDELATRRAEQDQKGWQPANREREVSFALKVFGHFATSADKGAVRDKTLLK

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=614, Percent_Identity=77.6872964169381, Blast_Score=1011, Evalue=0.0,
Organism=Escherichia coli, GI2367371, Length=560, Percent_Identity=30.3571428571429, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1788157, Length=542, Percent_Identity=29.3357933579336, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI1786464, Length=523, Percent_Identity=31.7399617590822, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6322476, Length=605, Percent_Identity=36.8595041322314, Blast_Score=346, Evalue=5e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 65815; Mature: 65684

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFTQFVPGHVHLKDMGQLV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCIS
HHHHHHHCCCHHHCCEEEECCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLIRLDLVDAMIEAADPNVSDER
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
IDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRKELFLKAGRQIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHH
ELCKRYYEQDDASVLPRSIGTFDAFENAMSLDIAMGGSSNTVLHLLAAAQEAGMDFKMED
HHHHHHHCCCCHHHCCHHCCCHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHH
IDRLSRKVPCLSKIAPNTNKYHMEDVHRAGGIMGLLGELDRAGLIHKNTHTVLGMSMGEQ
HHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCEECCCCEEEECHHHHH
LDQYDIIRNQDEELHKFFRAGPAGIRTTQAFSQDCRWDTVDNDRVNGCIRNKENAISQEG
HHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHCCCC
GLAVLFGNLAEDGCIVKTAGVDESIWKFTGTAIVFESQEDAVAGILGGKVKEGHVVVIRY
CEEEEECCCCCCCEEEEECCCCHHHHHEECCEEEEECCCCHHHHHHCCCCCCCCEEEEEE
EGPKGGPGMQEMLYPTSYLKSMGLGKKCALLTDGRFSGGTSGLSIGHASPEAASGGAIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
VRDGDIINIDIPNRAINLEISNDELATRRAEQDQKGWQPANREREVSFALKVFGHFATSA
EECCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
DKGAVRDKTLLK
CCCCCCCHHCCC
>Mature Secondary Structure 
PKLRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFTQFVPGHVHLKDMGQLV
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCIS
HHHHHHHCCCHHHCCEEEECCCEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLIRLDLVDAMIEAADPNVSDER
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
IDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSMLATHADRKELFLKAGRQIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHH
ELCKRYYEQDDASVLPRSIGTFDAFENAMSLDIAMGGSSNTVLHLLAAAQEAGMDFKMED
HHHHHHHCCCCHHHCCHHCCCHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHH
IDRLSRKVPCLSKIAPNTNKYHMEDVHRAGGIMGLLGELDRAGLIHKNTHTVLGMSMGEQ
HHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCEECCCCEEEECHHHHH
LDQYDIIRNQDEELHKFFRAGPAGIRTTQAFSQDCRWDTVDNDRVNGCIRNKENAISQEG
HHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHCCCC
GLAVLFGNLAEDGCIVKTAGVDESIWKFTGTAIVFESQEDAVAGILGGKVKEGHVVVIRY
CEEEEECCCCCCCEEEEECCCCHHHHHEECCEEEEECCCCHHHHHHCCCCCCCCEEEEEE
EGPKGGPGMQEMLYPTSYLKSMGLGKKCALLTDGRFSGGTSGLSIGHASPEAASGGAIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
VRDGDIINIDIPNRAINLEISNDELATRRAEQDQKGWQPANREREVSFALKVFGHFATSA
EECCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
DKGAVRDKTLLK
CCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA