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The map label for this gene is tpiA

Identifier: 148827853

GI number: 148827853

Start: 1216412

End: 1217203

Strand: Direct

Name: tpiA

Synonym: CGSHiGG_06675

Alternate gene names: 148827853

Gene position: 1216412-1217203 (Clockwise)

Preceding gene: 148827851

Following gene: 148827857

Centisome position: 64.46

GC content: 42.8

Gene sequence:

>792_bases
ATGGCACGTCGTCCTTTAGTTATGGGTAACTGGAAATTAAACGGTAGCAAAGCATTCACGAAAGAATTAATCGAGGGATT
AAAAGCGGAATTACATGATGTAACAGGTTGTGATGTAGCAATTGCCCCCCCCGTTATGTATTTAGGTACGGCTGAAGCAG
CACTTTCTGGTTGTGGTTGCAGCTGTGGCGGTAAAAGTGTGATTCAATTAGGTGCACAAAACGTAGATATCAATGTGAAA
GGTGCATTTACTGGTGATATTTCTAGCGAAATGTTAAAAGATTTCGGTGCAAAATATATCATTATTGGCCACTCTGAACG
CCGCACTTATCATAAAGAAAGCGACGAATTTGTGGCGAAAAAATTTGGTGCATTAAAAGAAGCTGGTTTAGTGCCAGTAT
TATGTATTGGTGAATCTGAAGCAGAAAACGAAGCAGGCAAAACAGAAGAAGTATGTGCGCGTCAAATTGATGCAGTCATC
AATGCATTAGGCGTAGAAGCATTCAACGGTGCAGTGATCGCATACGAACCAATCTGGGCAATTGGCACGGGTAAATCTGC
AACTCCAGCACAAGCACAAGCCGTTCATGCATTTATCCGTGGTCACATCGCAGCGAAATCACAAGCCGTGGCAGAGCAAG
TAATTATTCAATACGGCGGTTCTGTAAATGATGCAAATGCCGCTGAATTATTTACTCAGCCAGACATCGATGGTGCATTA
GTGGGTGGCGCATCATTAAAAGCCCCTGCATTTGCAGTAATTGTTAAAGCAGCAGCTGCAGCGAAAAACTAA

Upstream 100 bases:

>100_bases
TCTGTGAGAATTTTTTTAATTTTATGATCTTCTGCAAAGAATAGGGGCGAAATTTTGGTATAGTAAGCAAGTTAATTTTA
TCAACTATGGAGAAACAAAA

Downstream 100 bases:

>100_bases
TTTTCAGTTAATTAGAATAACAACAAAAGTGCGGTAAATTTTTACCGCACTTTTTTATATCTTATATTTTTGATGAATGG
GTTAGTAAGTGGTATCAATC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGCSCGGKSVIQLGAQNVDINVK
GAFTGDISSEMLKDFGAKYIIIGHSERRTYHKESDEFVAKKFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
NALGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGAL
VGGASLKAPAFAVIVKAAAAAKN

Sequences:

>Translated_263_residues
MARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGCSCGGKSVIQLGAQNVDINVK
GAFTGDISSEMLKDFGAKYIIIGHSERRTYHKESDEFVAKKFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
NALGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGAL
VGGASLKAPAFAVIVKAAAAAKN
>Mature_262_residues
ARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGCSCGGKSVIQLGAQNVDINVKG
AFTGDISSEMLKDFGAKYIIIGHSERRTYHKESDEFVAKKFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVIN
ALGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGALV
GGASLKAPAFAVIVKAAAAAKN

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=257, Percent_Identity=45.136186770428, Blast_Score=186, Evalue=1e-47,
Organism=Homo sapiens, GI226529917, Length=257, Percent_Identity=45.136186770428, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1790353, Length=261, Percent_Identity=72.4137931034483, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17536593, Length=258, Percent_Identity=47.6744186046512, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320255, Length=259, Percent_Identity=44.4015444015444, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28572008, Length=258, Percent_Identity=47.2868217054264, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28572006, Length=258, Percent_Identity=47.2868217054264, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI28572004, Length=258, Percent_Identity=47.2868217054264, Blast_Score=199, Evalue=1e-51,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_HAEI8 (Q4QMN8)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248369.1
- HSSP:   P50921
- ProteinModelPortal:   Q4QMN8
- SMR:   Q4QMN8
- STRING:   Q4QMN8
- GeneID:   3430110
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0800
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q4QMN8
- ProtClustDB:   PRK00042
- BioCyc:   HINF281310:NTHI0800-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27273; Mature: 27142

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 104-104 ACT_SITE 176-176 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGC
CCCCCEEEECEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCHHHHHHCCCC
SCGGKSVIQLGAQNVDINVKGAFTGDISSEMLKDFGAKYIIIGHSERRTYHKESDEFVAK
CCCCHHHHHCCCCCEEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
KFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVINALGVEAFNGAVIAYEPIWA
HHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEE
IGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGAL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEE
VGGASLKAPAFAVIVKAAAAAKN
ECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARRPLVMGNWKLNGSKAFTKELIEGLKAELHDVTGCDVAIAPPVMYLGTAEAALSGCGC
CCCCEEEECEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCHHHHHHCCCC
SCGGKSVIQLGAQNVDINVKGAFTGDISSEMLKDFGAKYIIIGHSERRTYHKESDEFVAK
CCCCHHHHHCCCCCEEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
KFGALKEAGLVPVLCIGESEAENEAGKTEEVCARQIDAVINALGVEAFNGAVIAYEPIWA
HHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEE
IGTGKSATPAQAQAVHAFIRGHIAAKSQAVAEQVIIQYGGSVNDANAAELFTQPDIDGAL
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEE
VGGASLKAPAFAVIVKAAAAAKN
ECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA