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The map label for this gene is waaA [H]

Identifier: 148827830

GI number: 148827830

Start: 1193071

End: 1194354

Strand: Reverse

Name: waaA [H]

Synonym: CGSHiGG_06540

Alternate gene names: 148827830

Gene position: 1194354-1193071 (Counterclockwise)

Preceding gene: 148827832

Following gene: 148827829

Centisome position: 63.29

GC content: 39.72

Gene sequence:

>1284_bases
ATGTGGCGTTTTTTTTATACCAGCTTGCTTTTAATTTGCCAGCCGTTGATATTGTGTTTTATTGGCTTGCTCAGTGTTAA
ATCTCCACGTTATCGCCAGCGTTTGGCTGAACGTTATGGTTTTTATGGTAATGCGCCTTGTCCACCACCACAAGGGATCT
TTATCCACGCAGCCTCCGTAGGGGAAGTGATTGCGGCGACACCGCTGGTTCGCCAATTGCAACAAGATTATCCGCATTTA
TCCATTACTTTTACAACTTTTACTCCCACAGGTTCAGAACGCGTAAAAGCCACTTTTGGAAACAGTGTTTTTCATTATTA
TTTACCCTTTGATTTACCTTTTTCGATCCATCGTTTTATTAATTTTGTACAACCTAAATTATGTATCGTGATGGAAACAG
AACTTTGGCCTAATCTTATTCATCAATTATTTTTACGCAATATCCCATTTGTTATCGCCAATGCACGTTTATCCGCGCGT
TCTGCACATCGTTACGGAAAAATAAAAGCACGCTTACAAACAATGTGGTCGCAAATTAGCTTGATTGCAGCACAAGATCA
TATTAGCGGAAAACGCTATGCCACTTTGGGATATCCAAAGGAAAAATTGAATATCACGGGCAATATTAAATATGATCTCT
CGATCACTGATGAATTACGTGAAAAGATTGATGATTTACGATCTCTTTGGGTAAAAAATCGTCCAATTTGGATTGCAGCC
AGTACGCATAACGGAGAAGATGAAATCATTTTAAAATCTCACCGCGCTTTGTTAGCAAAATACCCAAATTTATTGTTATT
GCTCGTTCCTCGCCATCCAGAGCGTTTTAATGTCGTGGCTGATTTACTCAAAAAGGAAAAATTCCAATTTATTCGTCGTT
CCACAAATGAATTACCAAATGAAAATACCCAAGTGATTTTGGGCGACAGTATGGGCGAGCTTATGTTGATGTATGGCATT
TCCGATATTGCTTTTGTGGGGGGAAGCCTTGTGAAACACGGGGGACACAATCCACTGGAACCTCTTGCGTTTAAAATGCC
AGTGATTACTGGCAAGCATACTTTTAATTTCCCTGAGATATTTAGAATGTTGGTGGAAGTGCAAGGTGTGTTGGAAGTTA
ATTCAACCGCAGATGCTTTGGAACGTGCGGTAGAAGCCTTATTAAATTCAAAAGAGGCTAGCGAACGTTTAGGCAATGCT
GGCTATGAAGTGCTGATGGAAAACCGTGGTGCATTACAGCGTTTGTTAGATTTATTAAAACCTTATTTGGAGCGTAATGT
ATGA

Upstream 100 bases:

>100_bases
TCATTTTTTACAATCATCGCAACGCTAATTGTAGGCATATTTTCAATCCTTTTTTTGCTATACTAGCCGAAATTTTTTCA
AGTATAACCGAATTTCATTT

Downstream 100 bases:

>100_bases
CGAGCGTGATTTATCCTGGCACTTTTGACCCGATTACGAACGGTCATTTAGATATAATTGAAAGAAGTGCGGTAATTTTT
CCTCGTGTTTTAGTGGCGGT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MWRFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNAPCPPPQGIFIHAASVGEVIAATPLVRQLQQDYPHL
SITFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFINFVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSAR
SAHRYGKIKARLQTMWSQISLIAAQDHISGKRYATLGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAA
STHNGEDEIILKSHRALLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPNENTQVILGDSMGELMLMYGI
SDIAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEIFRMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNA
GYEVLMENRGALQRLLDLLKPYLERNV

Sequences:

>Translated_427_residues
MWRFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNAPCPPPQGIFIHAASVGEVIAATPLVRQLQQDYPHL
SITFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFINFVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSAR
SAHRYGKIKARLQTMWSQISLIAAQDHISGKRYATLGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAA
STHNGEDEIILKSHRALLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPNENTQVILGDSMGELMLMYGI
SDIAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEIFRMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNA
GYEVLMENRGALQRLLDLLKPYLERNV
>Mature_427_residues
MWRFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNAPCPPPQGIFIHAASVGEVIAATPLVRQLQQDYPHL
SITFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFINFVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSAR
SAHRYGKIKARLQTMWSQISLIAAQDHISGKRYATLGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAA
STHNGEDEIILKSHRALLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPNENTQVILGDSMGELMLMYGI
SDIAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEIFRMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNA
GYEVLMENRGALQRLLDLLKPYLERNV

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers a single molecule of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=423, Percent_Identity=51.063829787234, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48715; Mature: 48715

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNAPCPPPQGIFIHAASV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCH
GEVIAATPLVRQLQQDYPHLSITFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFI
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHH
NFVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSARSAHRYGKIKARLQTMWSQIS
HHCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LIAAQDHISGKRYATLGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAA
HHEEHHCCCCCEEEECCCCHHHEEEEEEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEE
STHNGEDEIILKSHRALLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPN
CCCCCCCCEEEHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ENTQVILGDSMGELMLMYGISDIAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEI
CCCEEEECCCHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCCHHEECCEEECCCCCCHHHH
FRMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNAGYEVLMENRGALQRLLDLLK
HHHHHHHHHHHEECCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCHHHHHHHHHHHH
PYLERNV
HHHHCCC
>Mature Secondary Structure
MWRFFYTSLLLICQPLILCFIGLLSVKSPRYRQRLAERYGFYGNAPCPPPQGIFIHAASV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCH
GEVIAATPLVRQLQQDYPHLSITFTTFTPTGSERVKATFGNSVFHYYLPFDLPFSIHRFI
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCEEEEEECCCCCHHHHHHH
NFVQPKLCIVMETELWPNLIHQLFLRNIPFVIANARLSARSAHRYGKIKARLQTMWSQIS
HHCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LIAAQDHISGKRYATLGYPKEKLNITGNIKYDLSITDELREKIDDLRSLWVKNRPIWIAA
HHEEHHCCCCCEEEECCCCHHHEEEEEEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEE
STHNGEDEIILKSHRALLAKYPNLLLLLVPRHPERFNVVADLLKKEKFQFIRRSTNELPN
CCCCCCCCEEEHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ENTQVILGDSMGELMLMYGISDIAFVGGSLVKHGGHNPLEPLAFKMPVITGKHTFNFPEI
CCCEEEECCCHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCCHHEECCEEECCCCCCHHHH
FRMLVEVQGVLEVNSTADALERAVEALLNSKEASERLGNAGYEVLMENRGALQRLLDLLK
HHHHHHHHHHHEECCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCHHHHHHHHHHHH
PYLERNV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952982; 7542800 [H]