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The map label for this gene is tag [H]

Identifier: 148827832

GI number: 148827832

Start: 1195178

End: 1195834

Strand: Reverse

Name: tag [H]

Synonym: CGSHiGG_06550

Alternate gene names: 148827832

Gene position: 1195834-1195178 (Counterclockwise)

Preceding gene: 148827833

Following gene: 148827830

Centisome position: 63.37

GC content: 38.51

Gene sequence:

>657_bases
ATGAAACTCACTGAAGCTATGACGCCGACCAAGCACCCTCGATCCACTTGGCTCGGAATATATCAAAACGTTTTATTTTG
TCAAGTTTTAAATATGCAACAAATAACCAGATGCCCTTGGGTTGGCGAACAACCTATTTATATTGATTATCATGATAAGG
AATGGGGAAAGCCTGAATTCGACAGCCAAAAGCTATTTGAAAAAATTTGTTTGGAAGGGCAGCAAGCAGGGCTTTCGTGG
ATTACGGTTTTGAAAAAACGTGAATCTTACCGTGTAGCCTTTCATCAATTTGATCCGAAAAAAATCGCAACAATGACCGC
ACTTGATATTGATGATTGTATGAAAAATGCAGGATTAATTCGCCATCGAGCCAAGTTAGAAGCCATTGTCAAAAATGCGA
AAGCTTATTTAGAGATGGAAAAGTGCGGTGAAAATTTCAGTGATTTTATTTGGTCATTTGTAAATCATAAACCGATTGTT
AATGATGTGCCTGATTTGCGAACTGTACCTGCTAAAACAGAAGCATCTAAAGCACTTTCTAAAGCCTTAAAAAAACGTGG
TTTCGTCTTTGTTGGCGAAACCACGTGTTATGCTTTTATGCAGTCCATGGGGTTAGTGGACGATCATTTAAATGATTGTC
CTTGCAAAACTCGCTAG

Upstream 100 bases:

>100_bases
AAGAAATTTTATTGTGAAGGTGGAAGAAAAATACTGGGGGAAAATTTTGATCAAAAAGGACTGAAACATTTTCCTTACTC
CTAAAAAGCGAAAAGCCTCA

Downstream 100 bases:

>100_bases
TGTTTATGTTGATCTCGCTCCCATAAATCGGCGTATTTTACAAAGGTTGAATGTGCTGATAACACCGCTAATAAAAATCC
TTGTTTACCATCTAAAAAAC

Product: DNA-3-methyladenine glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKLTEAMTPTKHPRSTWLGIYQNVLFCQVLNMQQITRCPWVGEQPIYIDYHDKEWGKPEFDSQKLFEKICLEGQQAGLSW
ITVLKKRESYRVAFHQFDPKKIATMTALDIDDCMKNAGLIRHRAKLEAIVKNAKAYLEMEKCGENFSDFIWSFVNHKPIV
NDVPDLRTVPAKTEASKALSKALKKRGFVFVGETTCYAFMQSMGLVDDHLNDCPCKTR

Sequences:

>Translated_218_residues
MKLTEAMTPTKHPRSTWLGIYQNVLFCQVLNMQQITRCPWVGEQPIYIDYHDKEWGKPEFDSQKLFEKICLEGQQAGLSW
ITVLKKRESYRVAFHQFDPKKIATMTALDIDDCMKNAGLIRHRAKLEAIVKNAKAYLEMEKCGENFSDFIWSFVNHKPIV
NDVPDLRTVPAKTEASKALSKALKKRGFVFVGETTCYAFMQSMGLVDDHLNDCPCKTR
>Mature_218_residues
MKLTEAMTPTKHPRSTWLGIYQNVLFCQVLNMQQITRCPWVGEQPIYIDYHDKEWGKPEFDSQKLFEKICLEGQQAGLSW
ITVLKKRESYRVAFHQFDPKKIATMTALDIDDCMKNAGLIRHRAKLEAIVKNAKAYLEMEKCGENFSDFIWSFVNHKPIV
NDVPDLRTVPAKTEASKALSKALKKRGFVFVGETTCYAFMQSMGLVDDHLNDCPCKTR

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=181, Percent_Identity=62.9834254143646, Blast_Score=252, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 25115; Mature: 25115

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTEAMTPTKHPRSTWLGIYQNVLFCQVLNMQQITRCPWVGEQPIYIDYHDKEWGKPEF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCEEEEECCCCCCCCCC
DSQKLFEKICLEGQQAGLSWITVLKKRESYRVAFHQFDPKKIATMTALDIDDCMKNAGLI
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHCCHHHHHHHHHHH
RHRAKLEAIVKNAKAYLEMEKCGENFSDFIWSFVNHKPIVNDVPDLRTVPAKTEASKALS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
KALKKRGFVFVGETTCYAFMQSMGLVDDHLNDCPCKTR
HHHHHCCCEEEEHHHHHHHHHHCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MKLTEAMTPTKHPRSTWLGIYQNVLFCQVLNMQQITRCPWVGEQPIYIDYHDKEWGKPEF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCEEEEECCCCCCCCCC
DSQKLFEKICLEGQQAGLSWITVLKKRESYRVAFHQFDPKKIATMTALDIDDCMKNAGLI
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHCCHHHHHHHHHHH
RHRAKLEAIVKNAKAYLEMEKCGENFSDFIWSFVNHKPIVNDVPDLRTVPAKTEASKALS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
KALKKRGFVFVGETTCYAFMQSMGLVDDHLNDCPCKTR
HHHHHCCCEEEEHHHHHHHHHHCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]