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The map label for this gene is pfkA

Identifier: 148824200

GI number: 148824200

Start: 3380532

End: 3381563

Strand: Reverse

Name: pfkA

Synonym: TBFG_13025

Alternate gene names: 148824200

Gene position: 3381563-3380532 (Counterclockwise)

Preceding gene: 148824201

Following gene: 148824199

Centisome position: 76.43

GC content: 65.5

Gene sequence:

>1032_bases
ATGCGGATTGGAGTTCTTACCGGAGGCGGCGACTGTCCCGGCCTTAACGCCGTCATCCGTGCGGTGGTGCGTACCTGCCA
CGCCCGATACGGCTCGTCGGTGGTCGGATTTCAGAACGGCTTTCGCGGGCTGCTGGAGAACCGCCGTGTTCAACTGCACA
ACGACGACCGCAATGACCGGCTGCTGGCCAAGGGCGGCACGATGCTGGGCACGGCCCGGGTGCACCCCGACAAACTGCGG
GCGGGGCTGCCCCAAATCATGCAGACCTTGGACGACAACGGGATCGACGTCCTGATCCCGATCGGCGGTGAGGGTACGCT
GACCGCCGCGAGTTGGCTCTCCGAAGAGAATGTGCCGGTGGTCGGGGTGCCAAAGACGATCGACAACGACATCGACTGCA
CCGATGTGACCTTCGGCCACGACACCGCGTTGACCGTGGCCACCGAGGCCATCGACCGGCTGCACAGCACCGCCGAATCC
CACGAGCGGGTGATGCTGGTGGAGGTGATGGGTAGGCATGCCGGCTGGATCGCGCTGAACGCCGGGCTGGCCTCCGGTGC
TCACATGACCCTGATTCCTGAGCAGCCGTTCGACATCGAAGAGGTGTGCCGACTGGTCAAAGGCCGTTTCCAGCGCGGGG
ACTCGCATTTCATCTGCGTGGTCGCCGAAGGCGCCAAACCGGCTCCGGGGACGATCATGCTGCGCGAGGGTGGTCTCGAC
GAGTTCGGGCACGAGCGTTTCACAGGTGTAGCGGCGCAGCTGGCGGTCGAGGTCGAGAAGCGCATCAACAAGGATGTCCG
GGTGACGGTGTTGGGCCACATCCAGCGGGGTGGTACTCCGACCGCCTACGACCGAGTGCTGGCCACCCGATTCGGGGTGA
ACGCCGCCGACGCCGCGCATGCGGGCGAGTACGGCCAGATGGTGACGTTGCGCGGCCAGGACATCGGTCGGGTTCCGCTG
GCGGATGCGGTTCGCAAACTCAAACTGGTGCCGCAGAGCCGTTACGACGACGCCGCCGCCTTCTTCGGTTGA

Upstream 100 bases:

>100_bases
TTTAGCGGCGAGCAGACACAGACTCGCATCCGGCGCCCCTCGCCCGTGCGATTCTGTGTCTGCTCGCGCCAGGTCGGCGG
CCGCGCGGCAAGATGAGGGT

Downstream 100 bases:

>100_bases
CGTCGGGTGCTCAAACTAGGATCGCGGCTATGACTGTTGCTGCCGGGGCAGCCAAGGCCGCAGGGGCTGAACTGCTCGAT
TACGACGAGGTCGTCGCGCG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRIGVLTGGGDCPGLNAVIRAVVRTCHARYGSSVVGFQNGFRGLLENRRVQLHNDDRNDRLLAKGGTMLGTARVHPDKLR
AGLPQIMQTLDDNGIDVLIPIGGEGTLTAASWLSEENVPVVGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAES
HERVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPFDIEEVCRLVKGRFQRGDSHFICVVAEGAKPAPGTIMLREGGLD
EFGHERFTGVAAQLAVEVEKRINKDVRVTVLGHIQRGGTPTAYDRVLATRFGVNAADAAHAGEYGQMVTLRGQDIGRVPL
ADAVRKLKLVPQSRYDDAAAFFG

Sequences:

>Translated_343_residues
MRIGVLTGGGDCPGLNAVIRAVVRTCHARYGSSVVGFQNGFRGLLENRRVQLHNDDRNDRLLAKGGTMLGTARVHPDKLR
AGLPQIMQTLDDNGIDVLIPIGGEGTLTAASWLSEENVPVVGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAES
HERVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPFDIEEVCRLVKGRFQRGDSHFICVVAEGAKPAPGTIMLREGGLD
EFGHERFTGVAAQLAVEVEKRINKDVRVTVLGHIQRGGTPTAYDRVLATRFGVNAADAAHAGEYGQMVTLRGQDIGRVPL
ADAVRKLKLVPQSRYDDAAAFFG
>Mature_343_residues
MRIGVLTGGGDCPGLNAVIRAVVRTCHARYGSSVVGFQNGFRGLLENRRVQLHNDDRNDRLLAKGGTMLGTARVHPDKLR
AGLPQIMQTLDDNGIDVLIPIGGEGTLTAASWLSEENVPVVGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAES
HERVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPFDIEEVCRLVKGRFQRGDSHFICVVAEGAKPAPGTIMLREGGLD
EFGHERFTGVAAQLAVEVEKRINKDVRVTVLGHIQRGGTPTAYDRVLATRFGVNAADAAHAGEYGQMVTLRGQDIGRVPL
ADAVRKLKLVPQSRYDDAAAFFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI48762920, Length=375, Percent_Identity=35.4666666666667, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI266453768, Length=375, Percent_Identity=34.6666666666667, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI266453748, Length=375, Percent_Identity=34.6666666666667, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI4505749, Length=375, Percent_Identity=34.6666666666667, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI266453619, Length=375, Percent_Identity=34.6666666666667, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI11321601, Length=375, Percent_Identity=33.3333333333333, Blast_Score=171, Evalue=9e-43,
Organism=Escherichia coli, GI1790350, Length=338, Percent_Identity=38.1656804733728, Blast_Score=215, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI25147584, Length=367, Percent_Identity=31.3351498637602, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17558788, Length=380, Percent_Identity=30.7894736842105, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI72003601, Length=308, Percent_Identity=26.6233766233766, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321679, Length=365, Percent_Identity=34.5205479452055, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323861, Length=364, Percent_Identity=34.6153846153846, Blast_Score=165, Evalue=8e-42,
Organism=Drosophila melanogaster, GI28573326, Length=375, Percent_Identity=33.8666666666667, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24652337, Length=375, Percent_Identity=34.1333333333333, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI17647809, Length=376, Percent_Identity=34.5744680851064, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_MYCBO (P65691)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856680.1
- ProteinModelPortal:   P65691
- SMR:   P65691
- EnsemblBacteria:   EBMYCT00000014970
- GeneID:   1093228
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3035c
- GeneTree:   EBGT00050000017326
- HOGENOM:   HBG405044
- OMA:   MVALRGT
- ProtClustDB:   PRK03202
- BioCyc:   MBOV233413:MB3035C-MONOMER
- BRENDA:   2.7.1.11
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02483

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 36881; Mature: 36881

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 128-128 BINDING 163-163 BINDING 267-267 BINDING 273-273 BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVLTGGGDCPGLNAVIRAVVRTCHARYGSSVVGFQNGFRGLLENRRVQLHNDDRNDR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHCCEEEEECCCCCCE
LLAKGGTMLGTARVHPDKLRAGLPQIMQTLDDNGIDVLIPIGGEGTLTAASWLSEENVPV
EEECCCCEEEEECCCHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCE
VGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAESHERVMLVEVMGRHAGWIALN
EECCHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEE
AGLASGAHMTLIPEQPFDIEEVCRLVKGRFQRGDSHFICVVAEGAKPAPGTIMLREGGLD
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCCC
EFGHERFTGVAAQLAVEVEKRINKDVRVTVLGHIQRGGTPTAYDRVLATRFGVNAADAAH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHC
AGEYGQMVTLRGQDIGRVPLADAVRKLKLVPQSRYDDAAAFFG
CCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure
MRIGVLTGGGDCPGLNAVIRAVVRTCHARYGSSVVGFQNGFRGLLENRRVQLHNDDRNDR
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHCCEEEEECCCCCCE
LLAKGGTMLGTARVHPDKLRAGLPQIMQTLDDNGIDVLIPIGGEGTLTAASWLSEENVPV
EEECCCCEEEEECCCHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCE
VGVPKTIDNDIDCTDVTFGHDTALTVATEAIDRLHSTAESHERVMLVEVMGRHAGWIALN
EECCHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEE
AGLASGAHMTLIPEQPFDIEEVCRLVKGRFQRGDSHFICVVAEGAKPAPGTIMLREGGLD
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCCC
EFGHERFTGVAAQLAVEVEKRINKDVRVTVLGHIQRGGTPTAYDRVLATRFGVNAADAAH
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHC
AGEYGQMVTLRGQDIGRVPLADAVRKLKLVPQSRYDDAAAFFG
CCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972