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The map label for this gene is gatB

Identifier: 148824199

GI number: 148824199

Start: 3378973

End: 3380502

Strand: Reverse

Name: gatB

Synonym: TBFG_13024

Alternate gene names: 148824199

Gene position: 3380502-3378973 (Counterclockwise)

Preceding gene: 148824200

Following gene: 148824197

Centisome position: 76.41

GC content: 65.1

Gene sequence:

>1530_bases
ATGACTGTTGCTGCCGGGGCAGCCAAGGCCGCAGGGGCTGAACTGCTCGATTACGACGAGGTCGTCGCGCGCTTCCAGCC
GGTACTCGGCCTCGAGGTGCACGTCGAGCTGTCCACTGCGACCAAGATGTTCTGTGGCTGCACCACCACATTCGGCGGCG
AGCCAAACACCCAGGTGTGTCCGGTGTGTCTGGGCTTGCCCGGTTCGCTGCCGGTGCTCAACCGGGCCGCCGTGGAGTCG
GCGATCCGCATCGGGCTGGCGCTGAACTGCGAGATCGTACCCTGGTGTCGCTTCGCCCGGAAGAACTACTTCTACCCCGA
CATGCCCAAGAACTACCAGATCTCACAGTACGACGAGCCGATCGCCATCAACGGCTACCTGGACGCGCCTTTGGAAGACG
GCACCACTTGGCGGGTGGAGATTGAGCGAGCTCACATGGAAGAAGACACCGGCAAGCTCACCCACATCGGCAGCGAGACG
GGCCGGATCCACGGTGCCACCGGTTCGCTGATCGACTACAACCGTGCCGGCGTGCCGCTCATCGAGATCGTCACCAAACC
CATCGTGGGCGCCGGTGCCCGGGCGCCGCAGATCGCCCGGTCCTATGTGACGGCGTTGCGGGATCTGCTGCGCGCATTAG
ATGTATCTGATGTCCGGATGGACCAGGGTTCGATGCGCTGTGACGCCAACGTGTCGCTGAAGCCGGCCGGGACGACCGAA
TTCGGCACCCGGACCGAGACCAAGAACGTCAACTCGCTGAAGAGCGTCGAAGTCGCCGTCCGCTACGAAATGCAGCGCCA
GGGCGCTATTTTGGCGTCTGGTGGTCGGATCACCCAGGAAACCAGACACTTTCACGAGGCCGGTTACACCAGCGCGGGCC
GCACCAAGGAGACCGCCGAGGACTATCGGTACTTCCCGGAGCCGGATTTGGAGCCTGTCGCGCCCAGTCGCGAGCTGGTC
GAGCGACTACGCCAGACGATCCCCGAATTGCCGTGGTTGAGCCGCAGGAGGATTCAGCAGGAGTGGGGTGTTTCCGACGA
GGTGATGCGCGATCTGGTCAACGCCGGCGCCGTCGAATTGGTTGCTGCCACCGTCGAGCACGGCGCGTCCAGCGAGGCGG
CGCGCGCCTGGTGGGGAAACTTCCTGGCGCAAAAGGCCAACGAGGCCGGCATCGGGCTGGACGAACTGGCCATCACTCCT
GCCCAGGTCGCAGCCGTGGTGGCATTGGTCGATGAGGGCAAGCTGTCCAACAGCTTGGCCCGCCAAGTCGTGGAGGGTGT
GCTGGCCGGTGAAGGTGAGCCCGAACAGGTGATGACTGCGAGAGGGTTGGCGTTGGTCCGCGACGACTCGTTGACCCAGG
CCGCGGTCGACGAGGCCCTGGCCGCAAATCCTGATGTGGCGGACAAGATTCGCGGCGGCAAGGTGGCCGCGGCCGGCGCG
ATTGTCGGTGCGGTGATGAAGGCGACCCGCGGACAGGCCGACGCGGCCCGAGTGCGCGAACTCGTTCTAGAGGCCTGCGG
GCAGGGTTAG

Upstream 100 bases:

>100_bases
CGGATGCGGTTCGCAAACTCAAACTGGTGCCGCAGAGCCGTTACGACGACGCCGCCGCCTTCTTCGGTTGACGTCGGGTG
CTCAAACTAGGATCGCGGCT

Downstream 100 bases:

>100_bases
TTGCCGTCCGCGGGTAGCGGTTCATCACCGTCAGGGGAGTTGCGACGATACACGTTGAGTGTCCCGATGGTGGCCGCGCG
GTCGTACGCATGGCGGAATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTVAAGAAKAAGAELLDYDEVVARFQPVLGLEVHVELSTATKMFCGCTTTFGGEPNTQVCPVCLGLPGSLPVLNRAAVES
AIRIGLALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEPIAINGYLDAPLEDGTTWRVEIERAHMEEDTGKLTHIGSET
GRIHGATGSLIDYNRAGVPLIEIVTKPIVGAGARAPQIARSYVTALRDLLRALDVSDVRMDQGSMRCDANVSLKPAGTTE
FGTRTETKNVNSLKSVEVAVRYEMQRQGAILASGGRITQETRHFHEAGYTSAGRTKETAEDYRYFPEPDLEPVAPSRELV
ERLRQTIPELPWLSRRRIQQEWGVSDEVMRDLVNAGAVELVAATVEHGASSEAARAWWGNFLAQKANEAGIGLDELAITP
AQVAAVVALVDEGKLSNSLARQVVEGVLAGEGEPEQVMTARGLALVRDDSLTQAAVDEALAANPDVADKIRGGKVAAAGA
IVGAVMKATRGQADAARVRELVLEACGQG

Sequences:

>Translated_509_residues
MTVAAGAAKAAGAELLDYDEVVARFQPVLGLEVHVELSTATKMFCGCTTTFGGEPNTQVCPVCLGLPGSLPVLNRAAVES
AIRIGLALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEPIAINGYLDAPLEDGTTWRVEIERAHMEEDTGKLTHIGSET
GRIHGATGSLIDYNRAGVPLIEIVTKPIVGAGARAPQIARSYVTALRDLLRALDVSDVRMDQGSMRCDANVSLKPAGTTE
FGTRTETKNVNSLKSVEVAVRYEMQRQGAILASGGRITQETRHFHEAGYTSAGRTKETAEDYRYFPEPDLEPVAPSRELV
ERLRQTIPELPWLSRRRIQQEWGVSDEVMRDLVNAGAVELVAATVEHGASSEAARAWWGNFLAQKANEAGIGLDELAITP
AQVAAVVALVDEGKLSNSLARQVVEGVLAGEGEPEQVMTARGLALVRDDSLTQAAVDEALAANPDVADKIRGGKVAAAGA
IVGAVMKATRGQADAARVRELVLEACGQG
>Mature_508_residues
TVAAGAAKAAGAELLDYDEVVARFQPVLGLEVHVELSTATKMFCGCTTTFGGEPNTQVCPVCLGLPGSLPVLNRAAVESA
IRIGLALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEPIAINGYLDAPLEDGTTWRVEIERAHMEEDTGKLTHIGSETG
RIHGATGSLIDYNRAGVPLIEIVTKPIVGAGARAPQIARSYVTALRDLLRALDVSDVRMDQGSMRCDANVSLKPAGTTEF
GTRTETKNVNSLKSVEVAVRYEMQRQGAILASGGRITQETRHFHEAGYTSAGRTKETAEDYRYFPEPDLEPVAPSRELVE
RLRQTIPELPWLSRRRIQQEWGVSDEVMRDLVNAGAVELVAATVEHGASSEAARAWWGNFLAQKANEAGIGLDELAITPA
QVAAVVALVDEGKLSNSLARQVVEGVLAGEGEPEQVMTARGLALVRDDSLTQAAVDEALAANPDVADKIRGGKVAAAGAI
VGAVMKATRGQADAARVRELVLEACGQG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=503, Percent_Identity=34.9900596421471, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI115534094, Length=293, Percent_Identity=42.6621160409556, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319391, Length=316, Percent_Identity=32.9113924050633, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI45550808, Length=507, Percent_Identity=30.9664694280079, Blast_Score=218, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_MYCBO (P64200)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856679.1
- ProteinModelPortal:   P64200
- SMR:   P64200
- EnsemblBacteria:   EBMYCT00000017228
- GeneID:   1092922
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3034c
- GeneTree:   EBGT00050000017594
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   MBOV233413:MB3034C-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54633; Mature: 54502

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01234 GATB; PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAAGAAKAAGAELLDYDEVVARFQPVLGLEVHVELSTATKMFCGCTTTFGGEPNTQVC
CCCCCCCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCC
PVCLGLPGSLPVLNRAAVESAIRIGLALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEP
CEEECCCCCCCHHHHHHHHHHHHHCEEECCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCC
IAINGYLDAPLEDGTTWRVEIERAHMEEDTGKLTHIGSETGRIHGATGSLIDYNRAGVPL
EEEECEECCCCCCCCEEEEEEEHHHCCCCCCCEEEECCCCCEEECCCCCEEECCCCCCHH
IEIVTKPIVGAGARAPQIARSYVTALRDLLRALDVSDVRMDQGSMRCDANVSLKPAGTTE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCCEECCCEEECCCCCCC
FGTRTETKNVNSLKSVEVAVRYEMQRQGAILASGGRITQETRHFHEAGYTSAGRTKETAE
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCCCCCCHHHHH
DYRYFPEPDLEPVAPSRELVERLRQTIPELPWLSRRRIQQEWGVSDEVMRDLVNAGAVEL
HCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHH
VAATVEHGASSEAARAWWGNFLAQKANEAGIGLDELAITPAQVAAVVALVDEGKLSNSLA
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHH
RQVVEGVLAGEGEPEQVMTARGLALVRDDSLTQAAVDEALAANPDVADKIRGGKVAAAGA
HHHHHHHHCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCHHHHH
IVGAVMKATRGQADAARVRELVLEACGQG
HHHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVAAGAAKAAGAELLDYDEVVARFQPVLGLEVHVELSTATKMFCGCTTTFGGEPNTQVC
CCCCCCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCC
PVCLGLPGSLPVLNRAAVESAIRIGLALNCEIVPWCRFARKNYFYPDMPKNYQISQYDEP
CEEECCCCCCCHHHHHHHHHHHHHCEEECCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCC
IAINGYLDAPLEDGTTWRVEIERAHMEEDTGKLTHIGSETGRIHGATGSLIDYNRAGVPL
EEEECEECCCCCCCCEEEEEEEHHHCCCCCCCEEEECCCCCEEECCCCCEEECCCCCCHH
IEIVTKPIVGAGARAPQIARSYVTALRDLLRALDVSDVRMDQGSMRCDANVSLKPAGTTE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCCEECCCEEECCCCCCC
FGTRTETKNVNSLKSVEVAVRYEMQRQGAILASGGRITQETRHFHEAGYTSAGRTKETAE
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCCCCCCHHHHH
DYRYFPEPDLEPVAPSRELVERLRQTIPELPWLSRRRIQQEWGVSDEVMRDLVNAGAVEL
HCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHH
VAATVEHGASSEAARAWWGNFLAQKANEAGIGLDELAITPAQVAAVVALVDEGKLSNSLA
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHH
RQVVEGVLAGEGEPEQVMTARGLALVRDDSLTQAAVDEALAANPDVADKIRGGKVAAAGA
HHHHHHHHCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCHHHHH
IVGAVMKATRGQADAARVRELVLEACGQG
HHHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972