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The map label for this gene is gltX

Identifier: 148824182

GI number: 148824182

Start: 3360514

End: 3361986

Strand: Reverse

Name: gltX

Synonym: TBFG_13007

Alternate gene names: 148824182

Gene position: 3361986-3360514 (Counterclockwise)

Preceding gene: 148824183

Following gene: 148824180

Centisome position: 75.99

GC content: 66.67

Gene sequence:

>1473_bases
GTGACCGCCACGGAAACAGTCCGGGTCCGATTCTGCCCTTCACCCACCGGAACTCCGCACGTCGGTCTGGTCCGCACCGC
GCTGTTCAACTGGGCCTACGCCCGGCACACCGGTGGCACCTTCGTGTTCCGCATCGAGGACACCGACGCCCAACGCGACA
GCGAGGAAAGCTATCTGGCGCTGCTGGACGCGCTGCGCTGGCTTGGCCTGGATTGGGATGAGGGGCCCGAGGTGGGTGGG
CCGTACGGGCCGTACCGGCAGTCGCAGCGCGCCGAAATCTACCGTGACGTGCTCGCCCGACTGCTCGCCGCGGGCGAGGC
CTACCACGCTTTCTCCACACCCGAGGAAGTGGAGGCCCGTCATGTCGCGGCCGGGCGCAATCCCAAGCTGGGTTACGACA
ATTTCGATCGTCACTTGACCGACGCGCAGCGTGCGGCGTACCTGGCGGAGGGCCGCCAGCCGGTGGTGCGGCTGCGGATG
CCCGATGACGACCTCGCTTGGAATGACCTGGTGCGCGGGCCCGTGACGTTCGCGGCTGGCTCCGTACCCGACTTCGCGTT
GACCCGAGCCAGCGGTGATCCGTTATACACCTTGGTCAACCCGTGCGACGACGCGCTGATGAAGATCACCCATGTGTTGC
GTGGCGAGGACCTGCTGCCGTCGACGCCGCGCCAGCTGGCGCTACATCAGGCGTTGATCAGGATCGGGGTAGCTGAGCGG
ATTCCGAAATTCGCGCACCTCCCAACGGTCTTGGGGGAGGGCACCAAGAAGCTCTCGAAGCGCGATCCGCAGTCGAACCT
GTTTGCCCACCGCGACCGCGGGTTCATCCCCGAAGGCCTGCTGAACTACCTCGCGTTGCTCGGCTGGTCCATAGCCGACG
ACCACGACCTGTTCGGTCTCGACGAGATGGTGGCCGCGTTCGACGTTGCCGACGTCAACTCCAGCCCGGCCCGGTTCGAC
CAGAAGAAGGCCGACGCGCTGAACGCCGAGCACATCCGGATGCTCGACGTGGGCGACTTCACGGTCAGGCTGCGCGACCA
TCTGGATACCCACGGCCATCACATCGCGTTGGATGAGGCGGCATTCGCCGCCGCCGCCGAGTTGGTGCAGACCCGCATCG
TGGTGCTCGGTGACGCGTGGGAGCTGTTGAAGTTCTTCAACGACGATCAGTATGTGATCGATCCCAAGGCCGCGGCCAAG
GAGCTAGGCCCAGACGGGGCCGCGGTGCTCGACGCGGCCCTGGCCGCGCTGACAAGTGTGACGGACTGGACGGCACCCCT
GATCGAGGCGGCCCTTAAGGACGCCTTGATCGAAGGTTTGGCGCTCAAACCCCGCAAGGCGTTCAGCCCCATCCGGGTGG
CCGCCACCGGCACGACCGTCAGCCCGCCGTTGTTTGAATCGCTGGAGCTGCTCGGTCGTGACCGCAGCATGCAGCGGCTG
CGCGCGGCGCGCCAGCTGGTCGGGCACGCGTGA

Upstream 100 bases:

>100_bases
CACCCGCGGGAGTCGGTCCCATCGAGGACGGCGACACCGTCTCGATCACCATCGAGGGAATCGGCACCCTCACCAATCCC
GTAGTCCGCAAAGGAAAGCC

Downstream 100 bases:

>100_bases
GCCTTGTGTGGCGAATCTTTGGTAGTCTGCACGTCGGCCCATAACAGCTGGCGGCAGCGGTTTTAGGCAGCCCATGGGGT
ATGGTGTAATTGGCAACACA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLALLDALRWLGLDWDEGPEVGG
PYGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEARHVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRM
PDDDLAWNDLVRGPVTFAAGSVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAER
IPKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGLDEMVAAFDVADVNSSPARFD
QKKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEAAFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAK
ELGPDGAAVLDAALAALTSVTDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRL
RAARQLVGHA

Sequences:

>Translated_490_residues
MTATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLALLDALRWLGLDWDEGPEVGG
PYGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEARHVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRM
PDDDLAWNDLVRGPVTFAAGSVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAER
IPKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGLDEMVAAFDVADVNSSPARFD
QKKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEAAFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAK
ELGPDGAAVLDAALAALTSVTDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRL
RAARQLVGHA
>Mature_489_residues
TATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLALLDALRWLGLDWDEGPEVGGP
YGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEARHVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRMP
DDDLAWNDLVRGPVTFAAGSVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAERI
PKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGLDEMVAAFDVADVNSSPARFDQ
KKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEAAFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAKE
LGPDGAAVLDAALAALTSVTDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRLR
AARQLVGHA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=331, Percent_Identity=38.0664652567976, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1788743, Length=488, Percent_Identity=34.4262295081967, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI226510925, Length=265, Percent_Identity=29.811320754717, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71993394, Length=495, Percent_Identity=29.4949494949495, Blast_Score=204, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=503, Percent_Identity=30.2186878727634, Blast_Score=208, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24665321, Length=496, Percent_Identity=32.0564516129032, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_MYCBO (P0A637)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856661.1
- ProteinModelPortal:   P0A637
- SMR:   P0A637
- EnsemblBacteria:   EBMYCT00000015176
- GeneID:   1094008
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3016c
- GeneTree:   EBGT00050000016746
- HOGENOM:   HBG628189
- OMA:   LPYVMGQ
- ProtClustDB:   PRK01406
- BioCyc:   MBOV233413:MB3016C-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53865; Mature: 53733

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 260-260

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLA
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
LLDALRWLGLDWDEGPEVGGPYGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEAR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
HVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRMPDDDLAWNDLVRGPVTFAAG
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHHCCC
SVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAER
CCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IPKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGL
CCHHHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCH
DEMVAAFDVADVNSSPARFDQKKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEA
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHCCCCCCEEEEHHH
AFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAKELGPDGAAVLDAALAALTSV
HHHHHHHHHHHHHEEECCHHHHHHHHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHHHH
TDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRL
HHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHH
RAARQLVGHA
HHHHHHHCCC
>Mature Secondary Structure 
TATETVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGTFVFRIEDTDAQRDSEESYLA
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
LLDALRWLGLDWDEGPEVGGPYGPYRQSQRAEIYRDVLARLLAAGEAYHAFSTPEEVEAR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHH
HVAAGRNPKLGYDNFDRHLTDAQRAAYLAEGRQPVVRLRMPDDDLAWNDLVRGPVTFAAG
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHHCCC
SVPDFALTRASGDPLYTLVNPCDDALMKITHVLRGEDLLPSTPRQLALHQALIRIGVAER
CCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IPKFAHLPTVLGEGTKKLSKRDPQSNLFAHRDRGFIPEGLLNYLALLGWSIADDHDLFGL
CCHHHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCH
DEMVAAFDVADVNSSPARFDQKKADALNAEHIRMLDVGDFTVRLRDHLDTHGHHIALDEA
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHCCCCCCEEEEHHH
AFAAAAELVQTRIVVLGDAWELLKFFNDDQYVIDPKAAAKELGPDGAAVLDAALAALTSV
HHHHHHHHHHHHHEEECCHHHHHHHHCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHHHH
TDWTAPLIEAALKDALIEGLALKPRKAFSPIRVAATGTTVSPPLFESLELLGRDRSMQRL
HHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHH
RAARQLVGHA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972