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The map label for this gene is dhaA [H]

Identifier: 148823774

GI number: 148823774

Start: 2916956

End: 2917858

Strand: Direct

Name: dhaA [H]

Synonym: TBFG_12599

Alternate gene names: 148823774

Gene position: 2916956-2917858 (Clockwise)

Preceding gene: 148823772

Following gene: 148823776

Centisome position: 65.93

GC content: 63.57

Gene sequence:

>903_bases
ATGACAGCATTCGGCGTCGAGCCCTACGGGCAGCCGAAGTACCTAGAAATCGCCGGGAAGCGCATGGCGTATATCGACGA
AGGCAAGGGTGACGCCATCGTCTTTCAGCACGGCAACCCCACGTCGTCTTACTTGTGGCGCAACATCATGCCGCACTTGG
AAGGGCTGGGCCGGCTGGTGGCCTGCGATCTGATCGGGATGGGCGCGTCGGACAAGCTCAGCCCATCGGGACCCGACCGC
TATAGCTATGGCGAGCAACGAGACTTTTTGTTCGCGCTCTGGGATGCGCTCGACCTCGGCGACCACGTGGTACTGGTGCT
GCACGACTGGGGCTCGGCGCTCGGCTTCGACTGGGCTAACCAGCATCGCGACCGAGTGCAGGGGATCGCGTTCATGGAAG
CGATCGTCACCCCGATGACGTGGGCGGACTGGCCGCCGGCCGTGCGGGGTGTGTTCCAGGGTTTCCGATCGCCTCAAGGC
GAGCCAATGGCGTTGGAGCACAACATCTTTGTCGAACGGGTGCTGCCCGGGGCGATCCTGCGACAGCTCAGCGACGAGGA
AATGAACCACTATCGGCGGCCATTCGTGAACGGCGGCGAGGACCGTCGCCCCACGTTGTCGTGGCCACGAAACCTTCCAA
TCGACGGTGAGCCCGCCGAGGTCGTCGCGTTGGTCAACGAGTACCGGAGCTGGCTCGAGGAAACCGACATGCCGAAACTG
TTCATCAACGCCGAGCCCGGCGCGATCATCACCGGCCGCATCCGTGACTATGTCAGGAGCTGGCCCAACCAGACCGAAAT
CACAGTGCCCGGCGTGCATTTCGTTCAGGAGGACAGCCCAGAGGAAATCGGTGCGGCCATAGCACAGTTCGTCCGGCGGC
TCCGGTCGGCGGCCGGCGTCTGA

Upstream 100 bases:

>100_bases
TTCGAACAACCGTTCTATACTTTGTCAACGCTGGCCGCTACCGAGCGCCGCACAGGATGTGATATGCCATCTCTGCCCGC
ACAGACAGGAGCCAGGCCTT

Downstream 100 bases:

>100_bases
CCGCAACCGGGCCTCATGCTAGGCCACCGGCGACCGACGGACTTCCCGCGCGAGCCGCTCCAAAAGCCTCAGCCGCTCGG
GGTGGTCGGCTCGTCAAACG

Product: haloalkane dehalogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTAFGVEPYGQPKYLEIAGKRMAYIDEGKGDAIVFQHGNPTSSYLWRNIMPHLEGLGRLVACDLIGMGASDKLSPSGPDR
YSYGEQRDFLFALWDALDLGDHVVLVLHDWGSALGFDWANQHRDRVQGIAFMEAIVTPMTWADWPPAVRGVFQGFRSPQG
EPMALEHNIFVERVLPGAILRQLSDEEMNHYRRPFVNGGEDRRPTLSWPRNLPIDGEPAEVVALVNEYRSWLEETDMPKL
FINAEPGAIITGRIRDYVRSWPNQTEITVPGVHFVQEDSPEEIGAAIAQFVRRLRSAAGV

Sequences:

>Translated_300_residues
MTAFGVEPYGQPKYLEIAGKRMAYIDEGKGDAIVFQHGNPTSSYLWRNIMPHLEGLGRLVACDLIGMGASDKLSPSGPDR
YSYGEQRDFLFALWDALDLGDHVVLVLHDWGSALGFDWANQHRDRVQGIAFMEAIVTPMTWADWPPAVRGVFQGFRSPQG
EPMALEHNIFVERVLPGAILRQLSDEEMNHYRRPFVNGGEDRRPTLSWPRNLPIDGEPAEVVALVNEYRSWLEETDMPKL
FINAEPGAIITGRIRDYVRSWPNQTEITVPGVHFVQEDSPEEIGAAIAQFVRRLRSAAGV
>Mature_299_residues
TAFGVEPYGQPKYLEIAGKRMAYIDEGKGDAIVFQHGNPTSSYLWRNIMPHLEGLGRLVACDLIGMGASDKLSPSGPDRY
SYGEQRDFLFALWDALDLGDHVVLVLHDWGSALGFDWANQHRDRVQGIAFMEAIVTPMTWADWPPAVRGVFQGFRSPQGE
PMALEHNIFVERVLPGAILRQLSDEEMNHYRRPFVNGGEDRRPTLSWPRNLPIDGEPAEVVALVNEYRSWLEETDMPKLF
INAEPGAIITGRIRDYVRSWPNQTEITVPGVHFVQEDSPEEIGAAIAQFVRRLRSAAGV

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 33729; Mature: 33597

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFGVEPYGQPKYLEIAGKRMAYIDEGKGDAIVFQHGNPTSSYLWRNIMPHLEGLGRLV
CCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
ACDLIGMGASDKLSPSGPDRYSYGEQRDFLFALWDALDLGDHVVLVLHDWGSALGFDWAN
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCHHH
QHRDRVQGIAFMEAIVTPMTWADWPPAVRGVFQGFRSPQGEPMALEHNIFVERVLPGAIL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCHHHH
RQLSDEEMNHYRRPFVNGGEDRRPTLSWPRNLPIDGEPAEVVALVNEYRSWLEETDMPKL
HHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
FINAEPGAIITGRIRDYVRSWPNQTEITVPGVHFVQEDSPEEIGAAIAQFVRRLRSAAGV
EEECCCCEEEEHHHHHHHHCCCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAFGVEPYGQPKYLEIAGKRMAYIDEGKGDAIVFQHGNPTSSYLWRNIMPHLEGLGRLV
CCCCCCCCCCCCEEEECCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
ACDLIGMGASDKLSPSGPDRYSYGEQRDFLFALWDALDLGDHVVLVLHDWGSALGFDWAN
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHCCCHHH
QHRDRVQGIAFMEAIVTPMTWADWPPAVRGVFQGFRSPQGEPMALEHNIFVERVLPGAIL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCHHHH
RQLSDEEMNHYRRPFVNGGEDRRPTLSWPRNLPIDGEPAEVVALVNEYRSWLEETDMPKL
HHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
FINAEPGAIITGRIRDYVRSWPNQTEITVPGVHFVQEDSPEEIGAAIAQFVRRLRSAAGV
EEECCCCEEEEHHHHHHHHCCCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]