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The map label for this gene is pafA [H]

Identifier: 148823311

GI number: 148823311

Start: 2371358

End: 2372716

Strand: Reverse

Name: pafA [H]

Synonym: TBFG_12132

Alternate gene names: 148823311

Gene position: 2372716-2371358 (Counterclockwise)

Preceding gene: 148823312

Following gene: 148823310

Centisome position: 53.63

GC content: 63.65

Gene sequence:

>1359_bases
GTGCAGCGTCGAATCATGGGCATCGAAACCGAGTTCGGTGTCACCTGCACATTTCACGGTCACCGTCGGCTGTCCCCGGA
CGAGGTGGCCCGCTACCTGTTTCGCCGTGTGGTGTCCTGGGGCCGCAGCTCCAACGTCTTCCTGCGTAACGGTGCGCGCC
TGTATCTCGACGTGGGCAGTCATCCCGAGTACGCCACCGCCGAATGCGACAGCCTGGTGCAGCTGGTCACCCATGACCGG
GCCGGTGAATGGGTGCTGGAAGACCTGCTCGTTGACGCCGAGCAGCGGCTGGCCGACGAGGGCATCGGCGGCGACATCTA
CCTGTTCAAGAACAACACCGATTCGGCGGGCAACTCCTACGGCTGCCACGAAAACTACCTGATCGTGCGGGCCGGTGAGT
TCTCCCGGATCTCCGACGTGCTGCTGCCCTTCCTGGTCACCCGCCAGCTGATCTGCGGGGCCGGCAAGGTGCTGCAGACC
CCCAAGGCCGCCACCTACTGCTTAAGCCAACGCGCCGAACACATTTGGGAGGGCGTCTCTAGCGCCACGACCCGATCCCG
CCCCATTATCAACACCCGCGACGAGCCACATGCCGACGCCGAGAAGTACCGGCGGCTGCACGTCATCGTCGGCGACTCCA
ACATGTCCGAGACCACCACCATGCTCAAGGTGGGCACCGCGGCGCTCGTGCTGGAAATGATCGAAAGTGGAGTCGCCTTC
CGTGATTTTTCCCTGGATAACCCGATCCGCGCCATCCGTGAGGTTAGCCATGATGTCACCGGTCGCCGGCCGGTGCGGTT
GGCCGGAGGCCGTCAGGCCAGCGCGCTGGACATCCAGCGGGAGTACTACACCCGCGCAGTCGAGCACTTGCAGACTCGGG
AGCCCAACGCGCAGATCGAGCAGGTCGTCGACCTGTGGGGCCGCCAACTCGATGCCGTCGAAAGCCAGGATTTCGCCAAG
GTCGACACCGAAATCGACTGGGTGATCAAACGCAAGCTGTTTCAGCGTTATCAGGACCGCTATGACATGGAGTTGTCCCA
CCCGAAGATCGCTCAGCTGGATCTGGCCTACCACGACATCAAGCGCGGACGCGGGATCTTTGATCTCCTGCAGCGCAAAG
GGTTGGCCGCGCGCGTCACCACCGACGAAGAAATCGCGGAGGCCGTCGATCAGCCCCCACAGACCACCCGGGCCCGGCTG
CGCGGTGAGTTCATCAGCGCCGCGCAGGAGGCGGGACGTGATTTCACCGTCGACTGGGTACATCTCAAACTCAACGACCA
GGCGCAGCGCACCGTGCTGTGCAAAGACCCGTTCCGGGCGGTCGATGAGCGGGTTAAGCGGCTGATCGCGAGCATGTAG

Upstream 100 bases:

>100_bases
CGGCGGGCTGCTGTTCGGAGTCCCCGGCCCGTCCGGCCCGGACGGGTAACGGGCGTGGCTATCGCGCCGCCGCCAACCAA
CAACCAGTACCCTCAAATAC

Downstream 100 bases:

>100_bases
CACCACCAATGGCGACCTCGAAAGTCGAACGGCTGGTCAATCTCGTCATAGCCTTGCTGTCCACTCGTGGCTACATCACT
GCGGAGAAGATCAGGTCCAG

Product: hypothetical protein

Products: NA

Alternate protein names: Proteasome accessory factor A; Pup-conjugating enzyme [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATAECDSLVQLVTHDR
AGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQT
PKAATYCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSETTTMLKVGTAALVLEMIESGVAF
RDFSLDNPIRAIREVSHDVTGRRPVRLAGGRQASALDIQREYYTRAVEHLQTREPNAQIEQVVDLWGRQLDAVESQDFAK
VDTEIDWVIKRKLFQRYQDRYDMELSHPKIAQLDLAYHDIKRGRGIFDLLQRKGLAARVTTDEEIAEAVDQPPQTTRARL
RGEFISAAQEAGRDFTVDWVHLKLNDQAQRTVLCKDPFRAVDERVKRLIASM

Sequences:

>Translated_452_residues
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATAECDSLVQLVTHDR
AGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQT
PKAATYCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSETTTMLKVGTAALVLEMIESGVAF
RDFSLDNPIRAIREVSHDVTGRRPVRLAGGRQASALDIQREYYTRAVEHLQTREPNAQIEQVVDLWGRQLDAVESQDFAK
VDTEIDWVIKRKLFQRYQDRYDMELSHPKIAQLDLAYHDIKRGRGIFDLLQRKGLAARVTTDEEIAEAVDQPPQTTRARL
RGEFISAAQEAGRDFTVDWVHLKLNDQAQRTVLCKDPFRAVDERVKRLIASM
>Mature_452_residues
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATAECDSLVQLVTHDR
AGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQT
PKAATYCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSETTTMLKVGTAALVLEMIESGVAF
RDFSLDNPIRAIREVSHDVTGRRPVRLAGGRQASALDIQREYYTRAVEHLQTREPNAQIEQVVDLWGRQLDAVESQDFAK
VDTEIDWVIKRKLFQRYQDRYDMELSHPKIAQLDLAYHDIKRGRGIFDLLQRKGLAARVTTDEEIAEAVDQPPQTTRARL
RGEFISAAQEAGRDFTVDWVHLKLNDQAQRTVLCKDPFRAVDERVKRLIASM

Specific function: Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup- conjugating enzyme subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022279
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 51385; Mature: 51385

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGS
CCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCC
HPEYATAECDSLVQLVTHDRAGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSY
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQTPKAATYCLSQRAEHIWEGVS
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHH
SATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSETTTMLKVGTAALVLEMIESGVAF
HHCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
RDFSLDNPIRAIREVSHDVTGRRPVRLAGGRQASALDIQREYYTRAVEHLQTREPNAQIE
EECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
QVVDLWGRQLDAVESQDFAKVDTEIDWVIKRKLFQRYQDRYDMELSHPKIAQLDLAYHDI
HHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KRGRGIFDLLQRKGLAARVTTDEEIAEAVDQPPQTTRARLRGEFISAAQEAGRDFTVDWV
HHCCCHHHHHHCCCCEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
HLKLNDQAQRTVLCKDPFRAVDERVKRLIASM
EEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGS
CCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCC
HPEYATAECDSLVQLVTHDRAGEWVLEDLLVDAEQRLADEGIGGDIYLFKNNTDSAGNSY
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQTPKAATYCLSQRAEHIWEGVS
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHH
SATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMSETTTMLKVGTAALVLEMIESGVAF
HHCCCCCCCCCCCCCCCCCHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
RDFSLDNPIRAIREVSHDVTGRRPVRLAGGRQASALDIQREYYTRAVEHLQTREPNAQIE
EECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
QVVDLWGRQLDAVESQDFAKVDTEIDWVIKRKLFQRYQDRYDMELSHPKIAQLDLAYHDI
HHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KRGRGIFDLLQRKGLAARVTTDEEIAEAVDQPPQTTRARLRGEFISAAQEAGRDFTVDWV
HHCCCHHHHHHCCCCEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
HLKLNDQAQRTVLCKDPFRAVDERVKRLIASM
EEEECCCCCCEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA