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The map label for this gene is murI

Identifier: 148661127

GI number: 148661127

Start: 1506385

End: 1507200

Strand: Direct

Name: murI

Synonym: MRA_1346

Alternate gene names: 148661127

Gene position: 1506385-1507200 (Clockwise)

Preceding gene: 148661126

Following gene: 148661128

Centisome position: 34.08

GC content: 66.05

Gene sequence:

>816_bases
ATGAATTCGCCGTTGGCGCCCGTCGGAGTCTTTGATTCCGGCGTCGGGGGACTGACGGTCGCGCGGGCCATCATCGACCA
ACTGCCCGACGAGGACATCGTCTACGTCGGCGACACCGGTAACGGCCCGTACGGTCCGCTGACCATCCCGGAGATCCGGG
CGCACGCGCTGGCCATCGGCGACGATCTGGTCGGCCGAGGCGTCAAGGCGTTGGTGATCGCCTGCAACTCGGCGTCGTCG
GCGTGCCTGCGGGATGCTCGCGAGCGCTACCAGGTGCCCGTCGTCGAAGTGATACTGCCGGCGGTGCGGCGTGCGGTGGC
CGCCACCCGCAACGGCCGCATCGGGGTAATCGGCACGCGGGCGACCATCACTTCACACGCCTATCAGGACGCGTTCGCTG
CGGCCCGCGACACCGAAATCACCGCGGTGGCTTGCCCTCGCTTCGTGGACTTCGTCGAGCGCGGCGTCACCAGCGGTCGT
CAGGTGCTCGGTCTGGCGCAGGGCTACCTGGAACCGCTGCAGCGCGCCGAGGTCGACACGCTAGTGCTGGGCTGTACGCA
CTATCCACTGCTGTCCGGACTGATTCAACTGGCGATGGGCGAGAACGTCACGCTGGTCTCCAGCGCCGAGGAGACCGCTA
AGGAAGTGGTCCGGGTGCTCACCGAGATCGACTTATTGCGTCCGCATGACGCGCCGCCGGCAACTCGGATATTTGAAGCT
ACGGGCGACCCCGAAGCGTTTACCAAATTGGCCGCACGATTCCTGGGTCCGGTGCTCGGTGGTGTGCAACCCGTTCACCC
ATCGCGCATTCATTAG

Upstream 100 bases:

>100_bases
GTGCGGTTGCTGGCGTCGTGGCGGCGTATCTGTTGTCCGCTCCGGAGCGTAAGGCCCGTGCACTGAAAAGGGCCGGCGCG
CGTTCCGGGCATCCGAAGTT

Downstream 100 bases:

>100_bases
GCCATGGAAGAGATTCTCGTCACCGAATGCGTCGATGTATTCCGCATCGTTGTATCGGGCATGGCACAGTAGTGTCCGTG
CGGATAACCGTGCTCGGATG

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNSPLAPVGVFDSGVGGLTVARAIIDQLPDEDIVYVGDTGNGPYGPLTIPEIRAHALAIGDDLVGRGVKALVIACNSASS
ACLRDARERYQVPVVEVILPAVRRAVAATRNGRIGVIGTRATITSHAYQDAFAAARDTEITAVACPRFVDFVERGVTSGR
QVLGLAQGYLEPLQRAEVDTLVLGCTHYPLLSGLIQLAMGENVTLVSSAEETAKEVVRVLTEIDLLRPHDAPPATRIFEA
TGDPEAFTKLAARFLGPVLGGVQPVHPSRIH

Sequences:

>Translated_271_residues
MNSPLAPVGVFDSGVGGLTVARAIIDQLPDEDIVYVGDTGNGPYGPLTIPEIRAHALAIGDDLVGRGVKALVIACNSASS
ACLRDARERYQVPVVEVILPAVRRAVAATRNGRIGVIGTRATITSHAYQDAFAAARDTEITAVACPRFVDFVERGVTSGR
QVLGLAQGYLEPLQRAEVDTLVLGCTHYPLLSGLIQLAMGENVTLVSSAEETAKEVVRVLTEIDLLRPHDAPPATRIFEA
TGDPEAFTKLAARFLGPVLGGVQPVHPSRIH
>Mature_271_residues
MNSPLAPVGVFDSGVGGLTVARAIIDQLPDEDIVYVGDTGNGPYGPLTIPEIRAHALAIGDDLVGRGVKALVIACNSASS
ACLRDARERYQVPVVEVILPAVRRAVAATRNGRIGVIGTRATITSHAYQDAFAAARDTEITAVACPRFVDFVERGVTSGR
QVLGLAQGYLEPLQRAEVDTLVLGCTHYPLLSGLIQLAMGENVTLVSSAEETAKEVVRVLTEIDLLRPHDAPPATRIFEA
TGDPEAFTKLAARFLGPVLGGVQPVHPSRIH

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=237, Percent_Identity=33.7552742616034, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_MYCBO (P63636)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855027.1
- ProteinModelPortal:   P63636
- SMR:   P63636
- EnsemblBacteria:   EBMYCT00000016297
- GeneID:   1090663
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1373
- GeneTree:   EBGT00050000016063
- HOGENOM:   HBG645102
- OMA:   CGHIPYG
- ProtClustDB:   PRK00865
- BioCyc:   MBOV233413:MB1373-MONOMER
- BRENDA:   5.1.1.3
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 28643; Mature: 28643

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPLAPVGVFDSGVGGLTVARAIIDQLPDEDIVYVGDTGNGPYGPLTIPEIRAHALAIG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHC
DDLVGRGVKALVIACNSASSACLRDARERYQVPVVEVILPAVRRAVAATRNGRIGVIGTR
HHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
ATITSHAYQDAFAAARDTEITAVACPRFVDFVERGVTSGRQVLGLAQGYLEPLQRAEVDT
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHH
LVLGCTHYPLLSGLIQLAMGENVTLVSSAEETAKEVVRVLTEIDLLRPHDAPPATRIFEA
EEECCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEC
TGDPEAFTKLAARFLGPVLGGVQPVHPSRIH
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNSPLAPVGVFDSGVGGLTVARAIIDQLPDEDIVYVGDTGNGPYGPLTIPEIRAHALAIG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHC
DDLVGRGVKALVIACNSASSACLRDARERYQVPVVEVILPAVRRAVAATRNGRIGVIGTR
HHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
ATITSHAYQDAFAAARDTEITAVACPRFVDFVERGVTSGRQVLGLAQGYLEPLQRAEVDT
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHH
LVLGCTHYPLLSGLIQLAMGENVTLVSSAEETAKEVVRVLTEIDLLRPHDAPPATRIFEA
EEECCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEC
TGDPEAFTKLAARFLGPVLGGVQPVHPSRIH
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972