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The map label for this gene is fadA4

Identifier: 148661112

GI number: 148661112

Start: 1487172

End: 1488341

Strand: Direct

Name: fadA4

Synonym: MRA_1331

Alternate gene names: 148661112

Gene position: 1487172-1488341 (Clockwise)

Preceding gene: 148661110

Following gene: 148661113

Centisome position: 33.65

GC content: 65.56

Gene sequence:

>1170_bases
GTGATTGTTGCTGGCGCGCGTACACCCATCGGCAAGTTGATGGGCTCCCTGAAGGATTTCAGCGCCAGCGAGCTGGGTGC
CATCGCCATTAAGGGCGCCCTGGAGAAGGCCAACGTGCCGGCGTCCTTGGTCGAGTACGTGATCATGGGCCAGGTGTTGA
CCGCGGGTGCCGGGCAAATGCCCGCACGGCAGGCGGCAGTGGCGGCCGGCATCGGTTGGGATGTCCCTGCGCTGACGATC
AACAAGATGTGCCTGTCCGGCATCGACGCAATCGCGCTGGCTGATCAACTCATTCGGGCCAGAGAGTTCGACGTGGTGGT
GGCCGGCGGTCAGGAGTCGATGACGAAGGCGCCCCACCTGTTGATGAATAGCCGGTCGGGTTACAAGTACGGCGACGTTA
CGGTTTTGGACCACATGGCCTACGACGGTCTGCACGACGTGTTCACCGATCAGCCGATGGGCGCGCTCACCGAGCAACGC
AACGACGTCGACATGTTCACCCGCTCCGAACAGGACGAGTACGCGGCTGCGTCCCACCAAAAGGCGGCCGCGGCATGGAA
GGACGGCGTATTCGCCGACGAGGTGATCCCGGTGAACATCCCGCAGCGCACGGGCGATCCACTGCAGTTCACCGAGGACG
AGGGGATCCGCGCCAACACCACCGCCGCCGCGCTGGCCGGTCTGAAGCCGGCGTTCCGTGGCGACGGCACCATCACCGCC
GGGTCGGCGTCACAGATCTCCGACGGTGCGGCCGCGGTGGTGGTCATGAACCAGGAAAAGGCCCAGGAACTGGGGCTGAC
CTGGCTAGCCGAGATCGGCGCCCACGGTGTGGTGGCCGGGCCGGATTCCACACTGCAATCGCAGCCGGCCAACGCGATCA
ACAAGGCGCTGGATCGCGAGGGCATCTCGGTGGACCAGCTCGACGTGGTGGAGATCAACGAGGCGTTCGCTGCGGTGGCA
TTGGCCTCGATACGCGAACTCGGGCTGAACCCCCAGATCGTCAACGTCAACGGTGGTGCGATTGCCGTCGGGCATCCCCT
CGGCATGTCAGGGACGCGAATCACGCTACATGCGGCGCTGCAGTTGGCACGCCGGGGATCGGGCGTCGGGGTTGCCGCAT
TGTGCGGGGCTGGCGGGCAGGGCGACGCACTGATATTGCGGGCCGGATAG

Upstream 100 bases:

>100_bases
TCGTCATCACACAACGGTAACCTGAAGGGAAAGAATCTGCTTCTCCGGGTCGGTCAGATCGGCTTTCGGGTGCGCTGAGG
AGGTAGTCATAACGACATCG

Downstream 100 bases:

>100_bases
CGGTTGAGGGGTCGGTGGCGGCCAGTGTGATCTTGGTCATACCAACCGATCGCGGTATGTCGGCTCCTGCCGCAGGGTCG
GCGCCACCGGGTGGATCGAT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MIVAGARTPIGKLMGSLKDFSASELGAIAIKGALEKANVPASLVEYVIMGQVLTAGAGQMPARQAAVAAGIGWDVPALTI
NKMCLSGIDAIALADQLIRAREFDVVVAGGQESMTKAPHLLMNSRSGYKYGDVTVLDHMAYDGLHDVFTDQPMGALTEQR
NDVDMFTRSEQDEYAAASHQKAAAAWKDGVFADEVIPVNIPQRTGDPLQFTEDEGIRANTTAAALAGLKPAFRGDGTITA
GSASQISDGAAAVVVMNQEKAQELGLTWLAEIGAHGVVAGPDSTLQSQPANAINKALDREGISVDQLDVVEINEAFAAVA
LASIRELGLNPQIVNVNGGAIAVGHPLGMSGTRITLHAALQLARRGSGVGVAALCGAGGQGDALILRAG

Sequences:

>Translated_389_residues
MIVAGARTPIGKLMGSLKDFSASELGAIAIKGALEKANVPASLVEYVIMGQVLTAGAGQMPARQAAVAAGIGWDVPALTI
NKMCLSGIDAIALADQLIRAREFDVVVAGGQESMTKAPHLLMNSRSGYKYGDVTVLDHMAYDGLHDVFTDQPMGALTEQR
NDVDMFTRSEQDEYAAASHQKAAAAWKDGVFADEVIPVNIPQRTGDPLQFTEDEGIRANTTAAALAGLKPAFRGDGTITA
GSASQISDGAAAVVVMNQEKAQELGLTWLAEIGAHGVVAGPDSTLQSQPANAINKALDREGISVDQLDVVEINEAFAAVA
LASIRELGLNPQIVNVNGGAIAVGHPLGMSGTRITLHAALQLARRGSGVGVAALCGAGGQGDALILRAG
>Mature_389_residues
MIVAGARTPIGKLMGSLKDFSASELGAIAIKGALEKANVPASLVEYVIMGQVLTAGAGQMPARQAAVAAGIGWDVPALTI
NKMCLSGIDAIALADQLIRAREFDVVVAGGQESMTKAPHLLMNSRSGYKYGDVTVLDHMAYDGLHDVFTDQPMGALTEQR
NDVDMFTRSEQDEYAAASHQKAAAAWKDGVFADEVIPVNIPQRTGDPLQFTEDEGIRANTTAAALAGLKPAFRGDGTITA
GSASQISDGAAAVVVMNQEKAQELGLTWLAEIGAHGVVAGPDSTLQSQPANAINKALDREGISVDQLDVVEINEAFAAVA
LASIRELGLNPQIVNVNGGAIAVGHPLGMSGTRITLHAALQLARRGSGVGVAALCGAGGQGDALILRAG

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=43.1876606683805, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI4557237, Length=388, Percent_Identity=39.9484536082474, Blast_Score=268, Evalue=9e-72,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=40.1028277634961, Blast_Score=258, Evalue=5e-69,
Organism=Homo sapiens, GI4501853, Length=392, Percent_Identity=37.2448979591837, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=31.2056737588652, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI194353979, Length=388, Percent_Identity=26.0309278350515, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI87082165, Length=386, Percent_Identity=44.8186528497409, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=44.5876288659794, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1787663, Length=394, Percent_Identity=37.3096446700508, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI48994986, Length=393, Percent_Identity=35.6234096692112, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788683, Length=429, Percent_Identity=31.4685314685315, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25147385, Length=387, Percent_Identity=40.3100775193798, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI133906874, Length=387, Percent_Identity=39.2764857881137, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=38.560411311054, Blast_Score=247, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI17535917, Length=396, Percent_Identity=34.8484848484849, Blast_Score=181, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=30.9692671394799, Blast_Score=165, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6325229, Length=395, Percent_Identity=41.2658227848101, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=35.8585858585859, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24655093, Length=389, Percent_Identity=43.1876606683805, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24640423, Length=387, Percent_Identity=40.8268733850129, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI17648125, Length=389, Percent_Identity=35.9897172236504, Blast_Score=212, Evalue=4e-55,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=31.8289786223278, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIL_MYCBO (P66927)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855012.1
- ProteinModelPortal:   P66927
- SMR:   P66927
- EnsemblBacteria:   EBMYCT00000017969
- GeneID:   1090649
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1358
- GeneTree:   EBGT00070000031751
- HOGENOM:   HBG370930
- OMA:   GNVAGPD
- ProtClustDB:   PRK05790
- BioCyc:   MBOV233413:MB1358-MONOMER
- BRENDA:   2.3.1.9
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.9

Molecular weight: Translated: 40081; Mature: 40081

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 84-84 ACT_SITE 345-345 ACT_SITE 375-375

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVAGARTPIGKLMGSLKDFSASELGAIAIKGALEKANVPASLVEYVIMGQVLTAGAGQM
CEECCCCCCHHHHHHHHHCCCCHHCCCEEEECHHHHCCCCHHHHHHHHHHHHHHCCCCCC
PARQAAVAAGIGWDVPALTINKMCLSGIDAIALADQLIRAREFDVVVAGGQESMTKAPHL
CHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHCCHH
LMNSRSGYKYGDVTVLDHMAYDGLHDVFTDQPMGALTEQRNDVDMFTRSEQDEYAAASHQ
EECCCCCCCCCCEEEEHHHHHCCHHHHHCCCCCHHHHHCCCCHHHHHCCCCHHHHHHHHH
KAAAAWKDGVFADEVIPVNIPQRTGDPLQFTEDEGIRANTTAAALAGLKPAFRGDGTITA
HHHHHHHCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCHHHHHHHCCCCCCCCCCCEEC
GSASQISDGAAAVVVMNQEKAQELGLTWLAEIGAHGVVAGPDSTLQSQPANAINKALDRE
CCCHHCCCCCEEEEEECHHHHHHHCHHHHHHHCCCCEEECCCCHHHCCCHHHHHHHHHHC
GISVDQLDVVEINEAFAAVALASIRELGLNPQIVNVNGGAIAVGHPLGMSGTRITLHAAL
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCEEHHHHHH
QLARRGSGVGVAALCGAGGQGDALILRAG
HHHHCCCCCCEEEEECCCCCCCEEEEECC
>Mature Secondary Structure
MIVAGARTPIGKLMGSLKDFSASELGAIAIKGALEKANVPASLVEYVIMGQVLTAGAGQM
CEECCCCCCHHHHHHHHHCCCCHHCCCEEEECHHHHCCCCHHHHHHHHHHHHHHCCCCCC
PARQAAVAAGIGWDVPALTINKMCLSGIDAIALADQLIRAREFDVVVAGGQESMTKAPHL
CHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHCCHH
LMNSRSGYKYGDVTVLDHMAYDGLHDVFTDQPMGALTEQRNDVDMFTRSEQDEYAAASHQ
EECCCCCCCCCCEEEEHHHHHCCHHHHHCCCCCHHHHHCCCCHHHHHCCCCHHHHHHHHH
KAAAAWKDGVFADEVIPVNIPQRTGDPLQFTEDEGIRANTTAAALAGLKPAFRGDGTITA
HHHHHHHCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCHHHHHHHCCCCCCCCCCCEEC
GSASQISDGAAAVVVMNQEKAQELGLTWLAEIGAHGVVAGPDSTLQSQPANAINKALDRE
CCCHHCCCCCEEEEEECHHHHHHHCHHHHHHHCCCCEEECCCCHHHCCCHHHHHHHHHHC
GISVDQLDVVEINEAFAAVALASIRELGLNPQIVNVNGGAIAVGHPLGMSGTRITLHAAL
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCEEHHHHHH
QLARRGSGVGVAALCGAGGQGDALILRAG
HHHHCCCCCCEEEEECCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972