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The map label for this gene is rho

Identifier: 148661085

GI number: 148661085

Start: 1454514

End: 1456322

Strand: Direct

Name: rho

Synonym: MRA_1305

Alternate gene names: 148661085

Gene position: 1454514-1456322 (Clockwise)

Preceding gene: 148661084

Following gene: 148661086

Centisome position: 32.91

GC content: 65.51

Gene sequence:

>1809_bases
GTGACCGATACGGACCTCATTACGGCTGGCGAAAGTACCGACGGCAAGCCGTCGGATGCCGCTGCCACAGATCCCCCAGA
CCTCAACGCCGACGAGCCGGCCGGCTCGCTGGCCACCATGGTGCTGCCCGAACTGCGTGCGCTGGCTAATCGAGCCGGCG
TGAAGGGAACATCGGGTATGCGGAAGAACGAACTGATCGCTGCGATTGAGGAGATCAGGCGACAGGCCAACGGCGCCCCA
GCCGTTGACCGGTCGGCTCAAGAGCACGACAAGGGCGACCGGCCGCCCAGTTCCGAGGCACCGGCCACCCAGGGGGAACA
GACCCCGACCGAACAGATCGATTCCCAAAGCCAACAGGTCCGCCCGGAGCGGCGCAGCGCCACCCGTGAAGCGGGACCCT
CCGGCTCCGGTGAGCGTGCGGGCACAGCCGCAGACGACACCGACAACCGCCAAGGCGGTCAACAGGACGCCAAGACCGAG
GAGCGTGGCACCGACGCGGGTGGCGACCAAGGGGGTGACCAGCAGGCTTCGGGCGGTCAGCAGGCGCGCGGCGACGAGGA
CGGAGAAGCGCGTCAGGGCCGGCGCGGACGCCGGTTCCGCGATCGGCGGCGCCGCGGTGAACGATCCGGCGACGGCGCCG
AGGCTGAACTGCGTGAGGACGACGTCGTCCAGCCGGTAGCCGGCATACTCGACGTCCTGGACAACTACGCGTTTGTGCGC
ACCTCCGGCTACCTACCCGGTCCGCACGACGTGTATGTGTCGATGAACATGGTGCGCAAGAACGGCATGCGCCGTGGTGA
TGCGGTGACCGGTGCGGTGCGGGTGCCCAAGGAAGGGGAGCAACCCAACCAGCGGCAGAAGTTCAACCCGCTGGTCCGCC
TGGACAGCATCAACGGCGGATCGGTCGAAGACGCCAAGAAGCGGCCCGAGTTCGGCAAACTGACGCCGTTGTACCCCAAC
CAGCGGCTTCGTCTGGAAACCAGTACCGAGCGGCTGACCACCCGGGTCATCGACCTCATCATGCCGATCGGCAAGGGTCA
ACGCGCGTTGATTGTGTCGCCGCCCAAAGCGGGCAAGACAACGATCCTGCAGGACATCGCCAACGCGATCACCAGGAACA
ACCCGGAATGCCACCTCATGGTCGTGCTCGTCGACGAGCGGCCTGAGGAGGTCACCGATATGCAGCGCTCGGTCAAAGGC
GAGGTCATCGCTTCAACTTTCGACCGGCCGCCGTCGGACCACACGTCGGTCGCCGAGCTGGCGATCGAACGCGCCAAGCG
GCTGGTGGAGCAAGGCAAGGACGTCGTGGTGCTGCTCGATTCAATCACCCGGCTAGGCCGCGCTTACAACAACGCGTCGC
CGGCGTCGGGCCGGATCCTGTCCGGTGGTGTCGATTCCACGGCGTTGTACCCGCCCAAGCGCTTCCTGGGGGCCGCGCGC
AACATCGAAGAGGGCGGGTCGCTGACCATCATCGCCACTGCGATGGTCGAGACCGGGTCCACTGGTGACACGGTCATTTT
CGAGGAGTTCAAGGGCACCGGCAACGCCGAGCTCAAGCTGGACCGCAAGATCGCCGAGCGGCGGGTTTTCCCTGCGGTCG
ACGTGAACCCTTCTGGAACCCGCAAGGACGAGCTACTGCTGTCGCCCGACGAGTTCGCTATTGTGCACAAGCTGCGCCGC
GTGCTATCGGGCCTGGATTCCCACCAGGCCATCGACCTGCTGATGTCGCAGCTGCGTAAGACGAAGAACAACTACGAATT
CCTTGTTCAGGTGTCCAAGACCACGCCAGGGTCCATGGACAGCGACTGA

Upstream 100 bases:

>100_bases
CGATCACCGTTGCAAAGTCGATGATTGGCGCACTCGGCGATTTGGCTGACTGCAACAAAACCCCGTATGACGTGATCAGC
GGGGGAAGGAAAGGAAATCC

Downstream 100 bases:

>100_bases
TCCGGCGAGACGGCTCGCCGGGAATGTCCGCACGCATCTCGGTGTTTGGGGTGATAGCGGTTGACCTGGCATAATCGATG
CTCAACGAGTTGGAACCGGA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MTDTDLITAGESTDGKPSDAAATDPPDLNADEPAGSLATMVLPELRALANRAGVKGTSGMRKNELIAAIEEIRRQANGAP
AVDRSAQEHDKGDRPPSSEAPATQGEQTPTEQIDSQSQQVRPERRSATREAGPSGSGERAGTAADDTDNRQGGQQDAKTE
ERGTDAGGDQGGDQQASGGQQARGDEDGEARQGRRGRRFRDRRRRGERSGDGAEAELREDDVVQPVAGILDVLDNYAFVR
TSGYLPGPHDVYVSMNMVRKNGMRRGDAVTGAVRVPKEGEQPNQRQKFNPLVRLDSINGGSVEDAKKRPEFGKLTPLYPN
QRLRLETSTERLTTRVIDLIMPIGKGQRALIVSPPKAGKTTILQDIANAITRNNPECHLMVVLVDERPEEVTDMQRSVKG
EVIASTFDRPPSDHTSVAELAIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPPKRFLGAAR
NIEEGGSLTIIATAMVETGSTGDTVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGTRKDELLLSPDEFAIVHKLRR
VLSGLDSHQAIDLLMSQLRKTKNNYEFLVQVSKTTPGSMDSD

Sequences:

>Translated_602_residues
MTDTDLITAGESTDGKPSDAAATDPPDLNADEPAGSLATMVLPELRALANRAGVKGTSGMRKNELIAAIEEIRRQANGAP
AVDRSAQEHDKGDRPPSSEAPATQGEQTPTEQIDSQSQQVRPERRSATREAGPSGSGERAGTAADDTDNRQGGQQDAKTE
ERGTDAGGDQGGDQQASGGQQARGDEDGEARQGRRGRRFRDRRRRGERSGDGAEAELREDDVVQPVAGILDVLDNYAFVR
TSGYLPGPHDVYVSMNMVRKNGMRRGDAVTGAVRVPKEGEQPNQRQKFNPLVRLDSINGGSVEDAKKRPEFGKLTPLYPN
QRLRLETSTERLTTRVIDLIMPIGKGQRALIVSPPKAGKTTILQDIANAITRNNPECHLMVVLVDERPEEVTDMQRSVKG
EVIASTFDRPPSDHTSVAELAIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPPKRFLGAAR
NIEEGGSLTIIATAMVETGSTGDTVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGTRKDELLLSPDEFAIVHKLRR
VLSGLDSHQAIDLLMSQLRKTKNNYEFLVQVSKTTPGSMDSD
>Mature_601_residues
TDTDLITAGESTDGKPSDAAATDPPDLNADEPAGSLATMVLPELRALANRAGVKGTSGMRKNELIAAIEEIRRQANGAPA
VDRSAQEHDKGDRPPSSEAPATQGEQTPTEQIDSQSQQVRPERRSATREAGPSGSGERAGTAADDTDNRQGGQQDAKTEE
RGTDAGGDQGGDQQASGGQQARGDEDGEARQGRRGRRFRDRRRRGERSGDGAEAELREDDVVQPVAGILDVLDNYAFVRT
SGYLPGPHDVYVSMNMVRKNGMRRGDAVTGAVRVPKEGEQPNQRQKFNPLVRLDSINGGSVEDAKKRPEFGKLTPLYPNQ
RLRLETSTERLTTRVIDLIMPIGKGQRALIVSPPKAGKTTILQDIANAITRNNPECHLMVVLVDERPEEVTDMQRSVKGE
VIASTFDRPPSDHTSVAELAIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPPKRFLGAARN
IEEGGSLTIIATAMVETGSTGDTVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGTRKDELLLSPDEFAIVHKLRRV
LSGLDSHQAIDLLMSQLRKTKNNYEFLVQVSKTTPGSMDSD

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family

Homologues:

Organism=Escherichia coli, GI1790217, Length=408, Percent_Identity=51.9607843137255, Blast_Score=418, Evalue=1e-118,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RHO_MYCBO (P66029)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_854983.1
- ProteinModelPortal:   P66029
- SMR:   P66029
- EnsemblBacteria:   EBMYCT00000016608
- GeneID:   1090638
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1329
- GeneTree:   EBGT00050000014996
- HOGENOM:   HBG335262
- OMA:   KNNPECH
- ProtClustDB:   PRK12678
- BioCyc:   MBOV233413:MB1329-MONOMER
- HAMAP:   MF_01884
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00382
- SMART:   SM00357
- SMART:   SM00959
- TIGRFAMs:   TIGR00767

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind; SSF68912 Rho_N

EC number: NA

Molecular weight: Translated: 65134; Mature: 65003

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTDLITAGESTDGKPSDAAATDPPDLNADEPAGSLATMVLPELRALANRAGVKGTSGM
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
RKNELIAAIEEIRRQANGAPAVDRSAQEHDKGDRPPSSEAPATQGEQTPTEQIDSQSQQV
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHC
RPERRSATREAGPSGSGERAGTAADDTDNRQGGQQDAKTEERGTDAGGDQGGDQQASGGQ
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHCCCCCCCCCCCCCCCCCCC
QARGDEDGEARQGRRGRRFRDRRRRGERSGDGAEAELREDDVVQPVAGILDVLDNYAFVR
CCCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
TSGYLPGPHDVYVSMNMVRKNGMRRGDAVTGAVRVPKEGEQPNQRQKFNPLVRLDSINGG
ECCCCCCCCEEEEEHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHCCCCEEEECCCCC
SVEDAKKRPEFGKLTPLYPNQRLRLETSTERLTTRVIDLIMPIGKGQRALIVSPPKAGKT
CCHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHH
TILQDIANAITRNNPECHLMVVLVDERPEEVTDMQRSVKGEVIASTFDRPPSDHTSVAEL
HHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHH
AIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPPKRFLGAAR
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHC
NIEEGGSLTIIATAMVETGSTGDTVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGT
CCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCC
RKDELLLSPDEFAIVHKLRRVLSGLDSHQAIDLLMSQLRKTKNNYEFLVQVSKTTPGSMD
CCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
SD
CC
>Mature Secondary Structure 
TDTDLITAGESTDGKPSDAAATDPPDLNADEPAGSLATMVLPELRALANRAGVKGTSGM
CCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
RKNELIAAIEEIRRQANGAPAVDRSAQEHDKGDRPPSSEAPATQGEQTPTEQIDSQSQQV
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHC
RPERRSATREAGPSGSGERAGTAADDTDNRQGGQQDAKTEERGTDAGGDQGGDQQASGGQ
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHCCCCCCCCCCCCCCCCCCC
QARGDEDGEARQGRRGRRFRDRRRRGERSGDGAEAELREDDVVQPVAGILDVLDNYAFVR
CCCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEE
TSGYLPGPHDVYVSMNMVRKNGMRRGDAVTGAVRVPKEGEQPNQRQKFNPLVRLDSINGG
ECCCCCCCCEEEEEHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHCCCCEEEECCCCC
SVEDAKKRPEFGKLTPLYPNQRLRLETSTERLTTRVIDLIMPIGKGQRALIVSPPKAGKT
CCHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHH
TILQDIANAITRNNPECHLMVVLVDERPEEVTDMQRSVKGEVIASTFDRPPSDHTSVAEL
HHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHH
AIERAKRLVEQGKDVVVLLDSITRLGRAYNNASPASGRILSGGVDSTALYPPKRFLGAAR
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHC
NIEEGGSLTIIATAMVETGSTGDTVIFEEFKGTGNAELKLDRKIAERRVFPAVDVNPSGT
CCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCC
RKDELLLSPDEFAIVHKLRRVLSGLDSHQAIDLLMSQLRKTKNNYEFLVQVSKTTPGSMD
CCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
SD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972