Click here to switch to the map view.

The map label for this gene is thrB

Identifier: 148661084

GI number: 148661084

Start: 1453307

End: 1454257

Strand: Direct

Name: thrB

Synonym: MRA_1304

Alternate gene names: 148661084

Gene position: 1453307-1454257 (Clockwise)

Preceding gene: 148661083

Following gene: 148661085

Centisome position: 32.88

GC content: 63.83

Gene sequence:

>951_bases
ATGGTGACTCAAGCATTGTTGCCTTCTGGGCTGGTGGCCAGTGCGGTGGTGGCGGCGTCCAGTGCAAACCTGGGCCCGGG
CTTCGACAGTGTCGGTTTGGCGCTGAGTCTCTACGACGAGATCATCGTCGAGACAACAGATTCCGGCTTGACGGTGACTG
TAGACGGCGAGGGCGGCGACCAGGTGCCGCTGGGCCCCGAGCACCTCGTGGTCCGCGCCGTGCAGCACGGGTTACAGGCA
GCGGGGGTCAGCGCCGCCGGCCTGGCGGTGCGCTGCCGCAACGCCATCCCGCACTCCCGCGGCCTCGGCTCCTCCGCGGC
AGCAGTTGTGGGCGGTCTTGCGGCCGTTAACGGTCTTGTCGTACAAACGGATTCGTCACCATCGAGCGATGCTGAGCTGA
TTCAGTTGGCTTCGGAGTTCGAGGGTCATCCCGACAACGCGGCGGCCGCGGTTTTGGGTGGTGCCGTGGTTTCGTGGACT
GACCACAGTGGTGACCGGCCCAACTATTCGGCCGTATCACTGCGGCTTCATCCCGATATCCGCCTGTTCACTGCGATTCC
CGAGCAGCGTTCGTCGACCGCGGAAACGCGGGTGCTATTGCCCGCGCAGGTTAGTCACGACGACGCACGGTTCAATGTCA
GTCGCGCGGCGCTGCTGGTGGTTGCGCTCACCGAACGGCCCGATCTGCTGATGGCGGCCACCGAAGATCTGCTTCATCAG
CCGCAACGTGCCGCGGCAATGACAGCCTCCGCGGAATATCTTCGGCTGTTGCGGCGTCATAACGTGGCAGCAGCACTGTC
CGGGGCAGGTCCTTCGTTGATCGCCCTGAGTACAGATTCAGAGTTGCCGACCGACGCCGTGGAGTTCGGAGCCGCAAAGG
GATTTGCCGTTACCGAGCTGACTGTTGGCGAGGCGGTTCGCTGGAGCCCGACAGTAAGAGTTCCCGGTTAA

Upstream 100 bases:

>100_bases
CGATCGCAAGCGCGGCGGAGCCGGGTGCGGCGGGTCGGCACGGTTTGGATTGGGTGGCGATCGCAAGCGCGGCGGAGCCG
GGTGCGGCGGGTCGGCACGC

Downstream 100 bases:

>100_bases
TCCGCAAGGTTGCGGGGGTTTGCTTGCTTCCGGCCAGGAAGCGGGCTATCCTCGGAGCCGTCCAGCAATCGCAGCATCTG
CATACGTACTGCCTTGCCGC

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MVTQALLPSGLVASAVVAASSANLGPGFDSVGLALSLYDEIIVETTDSGLTVTVDGEGGDQVPLGPEHLVVRAVQHGLQA
AGVSAAGLAVRCRNAIPHSRGLGSSAAAVVGGLAAVNGLVVQTDSSPSSDAELIQLASEFEGHPDNAAAAVLGGAVVSWT
DHSGDRPNYSAVSLRLHPDIRLFTAIPEQRSSTAETRVLLPAQVSHDDARFNVSRAALLVVALTERPDLLMAATEDLLHQ
PQRAAAMTASAEYLRLLRRHNVAAALSGAGPSLIALSTDSELPTDAVEFGAAKGFAVTELTVGEAVRWSPTVRVPG

Sequences:

>Translated_316_residues
MVTQALLPSGLVASAVVAASSANLGPGFDSVGLALSLYDEIIVETTDSGLTVTVDGEGGDQVPLGPEHLVVRAVQHGLQA
AGVSAAGLAVRCRNAIPHSRGLGSSAAAVVGGLAAVNGLVVQTDSSPSSDAELIQLASEFEGHPDNAAAAVLGGAVVSWT
DHSGDRPNYSAVSLRLHPDIRLFTAIPEQRSSTAETRVLLPAQVSHDDARFNVSRAALLVVALTERPDLLMAATEDLLHQ
PQRAAAMTASAEYLRLLRRHNVAAALSGAGPSLIALSTDSELPTDAVEFGAAKGFAVTELTVGEAVRWSPTVRVPG
>Mature_316_residues
MVTQALLPSGLVASAVVAASSANLGPGFDSVGLALSLYDEIIVETTDSGLTVTVDGEGGDQVPLGPEHLVVRAVQHGLQA
AGVSAAGLAVRCRNAIPHSRGLGSSAAAVVGGLAAVNGLVVQTDSSPSSDAELIQLASEFEGHPDNAAAAVLGGAVVSWT
DHSGDRPNYSAVSLRLHPDIRLFTAIPEQRSSTAETRVLLPAQVSHDDARFNVSRAALLVVALTERPDLLMAATEDLLHQ
PQRAAAMTASAEYLRLLRRHNVAAALSGAGPSLIALSTDSELPTDAVEFGAAKGFAVTELTVGEAVRWSPTVRVPG

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=281, Percent_Identity=28.8256227758007, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321814, Length=337, Percent_Identity=31.4540059347181, Blast_Score=119, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_MYCBO (P65225)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_854982.1
- ProteinModelPortal:   P65225
- SMR:   P65225
- EnsemblBacteria:   EBMYCT00000015809
- GeneID:   1090616
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1328
- GeneTree:   EBGT00050000016145
- HOGENOM:   HBG646290
- OMA:   FDCLGAA
- ProtClustDB:   PRK01212
- BioCyc:   MBOV233413:MB1328-MONOMER
- BRENDA:   2.7.1.39
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 32347; Mature: 32347

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00627 GHMP_KINASES_ATP; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTQALLPSGLVASAVVAASSANLGPGFDSVGLALSLYDEIIVETTDSGLTVTVDGEGGD
CCCHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEEEEECCCCC
QVPLGPEHLVVRAVQHGLQAAGVSAAGLAVRCRNAIPHSRGLGSSAAAVVGGLAAVNGLV
CCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEEHHCCCCCCCCCCHHHHHHHHHHHHCCEE
VQTDSSPSSDAELIQLASEFEGHPDNAAAAVLGGAVVSWTDHSGDRPNYSAVSLRLHPDI
EEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCCCCCCCCEEEEEEECCCE
RLFTAIPEQRSSTAETRVLLPAQVSHDDARFNVSRAALLVVALTERPDLLMAATEDLLHQ
EEEEECCCCCCCCCCEEEEEECCCCCCCCCEEHHHEEEEEEEECCCCCCHHHHHHHHHHC
PQRAAAMTASAEYLRLLRRHNVAAALSGAGPSLIALSTDSELPTDAVEFGAAKGFAVTEL
CHHHHHHHHHHHHHHHHHHCCHHEEECCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEE
TVGEAVRWSPTVRVPG
ECCCCEECCCCEECCC
>Mature Secondary Structure
MVTQALLPSGLVASAVVAASSANLGPGFDSVGLALSLYDEIIVETTDSGLTVTVDGEGGD
CCCHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEECCCCEEEEEECCCCC
QVPLGPEHLVVRAVQHGLQAAGVSAAGLAVRCRNAIPHSRGLGSSAAAVVGGLAAVNGLV
CCCCCHHHHHHHHHHHHHHHCCCCHHHHEEEEHHCCCCCCCCCCHHHHHHHHHHHHCCEE
VQTDSSPSSDAELIQLASEFEGHPDNAAAAVLGGAVVSWTDHSGDRPNYSAVSLRLHPDI
EEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCCCCCCCCEEEEEEECCCE
RLFTAIPEQRSSTAETRVLLPAQVSHDDARFNVSRAALLVVALTERPDLLMAATEDLLHQ
EEEEECCCCCCCCCCEEEEEECCCCCCCCCEEHHHEEEEEEEECCCCCCHHHHHHHHHHC
PQRAAAMTASAEYLRLLRRHNVAAALSGAGPSLIALSTDSELPTDAVEFGAAKGFAVTEL
CHHHHHHHHHHHHHHHHHHCCHHEEECCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEE
TVGEAVRWSPTVRVPG
ECCCCEECCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972