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The map label for this gene is thrA

Identifier: 148661082

GI number: 148661082

Start: 1450685

End: 1452010

Strand: Direct

Name: thrA

Synonym: MRA_1302

Alternate gene names: 148661082

Gene position: 1450685-1452010 (Clockwise)

Preceding gene: 148661081

Following gene: 148661083

Centisome position: 32.82

GC content: 65.38

Gene sequence:

>1326_bases
GTGCCCGGTGACGAAAAGCCGGTCGGCGTAGCGGTACTCGGTTTGGGCAACGTCGGCAGCGAGGTTGTCCGCATCATCGA
GAACAGCGCCGAGGATCTCGCGGCTCGTGTCGGTGCCCCATTGGTCCTGCGGGGCATCGGCGTGCGCCGCGTGACGACCG
ATCGCGGCGTGCCGATCGAATTGTTGACCGACGACATTGAAGAGCTCGTGGCCCGCGAGGATGTCGATATCGTGGTGGAA
GTGATGGGGCCGGTGGAACCGTCGCGCAAGGCGATCCTGGGCGCCCTTGAGCGCGGCAAGTCCGTCGTTACGGCGAACAA
GGCTTTACTCGCCACCTCCACCGGCGAATTGGCACAGGCCGCCGAAAGCGCCCATGTTGATCTGTATTTCGAGGCGGCCG
TGGCGGGCGCCATTCCGGTCATCCGTCCGCTCACCCAGTCGCTGGCCGGCGACACGGTGCTGCGAGTGGCCGGGATCGTC
AACGGCACCACCAACTACATCCTCTCGGCGATGGACAGCACCGGCGCTGACTATGCCAGCGCCCTGGCCGACGCAAGTGC
GCTGGGCTATGCGGAGGCTGATCCCACCGCAGACGTCGAAGGCTACGACGCCGCGGCCAAGGCAGCGATCCTGGCATCCA
TTGCCTTCCACACCCGGGTGACCGCAGACGACGTGTATCGCGAAGGCATCACCAAGGTCACTCCGGCCGACTTCGGATCC
GCGCACGCGCTGGGTTGCACCATCAAACTGCTGTCGATCTGTGAGCGCATAACCACCGACGAAGGTTCGCAGCGGGTATC
GGCCCGCGTCTATCCGGCCCTGGTACCTCTGTCGCATCCGCTTGCCGCGGTCAACGGCGCGTTCAATGCCGTGGTGGTCG
AGGCCGAGGCCGCGGGCCGGCTGATGTTCTACGGCCAGGGCGCGGGCGGCGCGCCGACCGCCTCTGCGGTGACCGGTGAC
CTAGTGATGGCCGCCCGCAACCGGGTACTCGGCAGCCGCGGCCCCCGTGAGTCTAAATACGCTCAACTTCCGGTGGCACC
AATGGGTTTCATTGAAACGCGCTATTACGTCAGCATGAACGTCGCCGACAAGCCGGGCGTCTTGTCCGCGGTGGCGGCGG
AATTCGCCAAACGCGAGGTGAGCATCGCCGAGGTGCGCCAGGAGGGCGTTGTGGACGAAGGTGGTCGACGGGTGGGAGCC
CGAATCGTGGTGGTCACGCACCTCGCCACTGACGCCGCACTCTCGGAAACCGTTGATGCACTGGACGACTTGGATGTCGT
GCAGGGTGTGTCCAGCGTGATACGACTGGAAGGAACCGGCTTATGA

Upstream 100 bases:

>100_bases
CGGCCGTCCCGCTGTGGTAGCGGTGCACGCGGGCAACGCTCGCCTGGTCCTGCGTCGGGAGACGGTCGACGATTTGCTGA
GTTTGGAAGTGAGGTGACCC

Downstream 100 bases:

>100_bases
CCGTCCCGCCGACGGCCACTCACCAGCCGTGGCCGGGAGTGATTGCCGCGTACCGTGACCGGCTGCCGGTGGGTGACGAC
TGGACTCCGGTGACCCTGCT

Product: homoserine dehydrogenase

Products: NA

Alternate protein names: HDH

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MPGDEKPVGVAVLGLGNVGSEVVRIIENSAEDLAARVGAPLVLRGIGVRRVTTDRGVPIELLTDDIEELVAREDVDIVVE
VMGPVEPSRKAILGALERGKSVVTANKALLATSTGELAQAAESAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIV
NGTTNYILSAMDSTGADYASALADASALGYAEADPTADVEGYDAAAKAAILASIAFHTRVTADDVYREGITKVTPADFGS
AHALGCTIKLLSICERITTDEGSQRVSARVYPALVPLSHPLAAVNGAFNAVVVEAEAAGRLMFYGQGAGGAPTASAVTGD
LVMAARNRVLGSRGPRESKYAQLPVAPMGFIETRYYVSMNVADKPGVLSAVAAEFAKREVSIAEVRQEGVVDEGGRRVGA
RIVVVTHLATDAALSETVDALDDLDVVQGVSSVIRLEGTGL

Sequences:

>Translated_441_residues
MPGDEKPVGVAVLGLGNVGSEVVRIIENSAEDLAARVGAPLVLRGIGVRRVTTDRGVPIELLTDDIEELVAREDVDIVVE
VMGPVEPSRKAILGALERGKSVVTANKALLATSTGELAQAAESAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIV
NGTTNYILSAMDSTGADYASALADASALGYAEADPTADVEGYDAAAKAAILASIAFHTRVTADDVYREGITKVTPADFGS
AHALGCTIKLLSICERITTDEGSQRVSARVYPALVPLSHPLAAVNGAFNAVVVEAEAAGRLMFYGQGAGGAPTASAVTGD
LVMAARNRVLGSRGPRESKYAQLPVAPMGFIETRYYVSMNVADKPGVLSAVAAEFAKREVSIAEVRQEGVVDEGGRRVGA
RIVVVTHLATDAALSETVDALDDLDVVQGVSSVIRLEGTGL
>Mature_440_residues
PGDEKPVGVAVLGLGNVGSEVVRIIENSAEDLAARVGAPLVLRGIGVRRVTTDRGVPIELLTDDIEELVAREDVDIVVEV
MGPVEPSRKAILGALERGKSVVTANKALLATSTGELAQAAESAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIVN
GTTNYILSAMDSTGADYASALADASALGYAEADPTADVEGYDAAAKAAILASIAFHTRVTADDVYREGITKVTPADFGSA
HALGCTIKLLSICERITTDEGSQRVSARVYPALVPLSHPLAAVNGAFNAVVVEAEAAGRLMFYGQGAGGAPTASAVTGDL
VMAARNRVLGSRGPRESKYAQLPVAPMGFIETRYYVSMNVADKPGVLSAVAAEFAKREVSIAEVRQEGVVDEGGRRVGAR
IVVVTHLATDAALSETVDALDDLDVVQGVSSVIRLEGTGL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0460

COG function: function code E; Homoserine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain

Homologues:

Organism=Escherichia coli, GI1786183, Length=249, Percent_Identity=29.718875502008, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790376, Length=360, Percent_Identity=25.5555555555556, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHOM_MYCBO (P63630)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_854980.1
- ProteinModelPortal:   P63630
- SMR:   P63630
- EnsemblBacteria:   EBMYCT00000015753
- GeneID:   1090613
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1326
- GeneTree:   EBGT00050000016352
- HOGENOM:   HBG527379
- OMA:   GIPIIEP
- ProtClustDB:   PRK06349
- BioCyc:   MBOV233413:MB1326-MONOMER
- BRENDA:   1.1.1.3
- InterPro:   IPR002912
- InterPro:   IPR005106
- InterPro:   IPR016204
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000098

Pfam domain/function: PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3

EC number: =1.1.1.3

Molecular weight: Translated: 45553; Mature: 45422

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01042 HOMOSER_DHGENASE

Important sites: ACT_SITE 207-207 BINDING 107-107 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGDEKPVGVAVLGLGNVGSEVVRIIENSAEDLAARVGAPLVLRGIGVRRVTTDRGVPIE
CCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHCCCEEEECCCEEEEECCCCCCHH
LLTDDIEELVAREDVDIVVEVMGPVEPSRKAILGALERGKSVVTANKALLATSTGELAQA
HHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCEEEEECCHHHHHH
AESAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIVNGTTNYILSAMDSTGADYAS
HHCCCEEEEEEHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHH
ALADASALGYAEADPTADVEGYDAAAKAAILASIAFHTRVTADDVYREGITKVTPADFGS
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCC
AHALGCTIKLLSICERITTDEGSQRVSARVYPALVPLSHPLAAVNGAFNAVVVEAEAAGR
CHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCEECCCCCCHHHHCCCCEEEEEEECCCCE
LMFYGQGAGGAPTASAVTGDLVMAARNRVLGSRGPRESKYAQLPVAPMGFIETRYYVSMN
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
VADKPGVLSAVAAEFAKREVSIAEVRQEGVVDEGGRRVGARIVVVTHLATDAALSETVDA
CCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEECEEEEEEEEHHHHHHHHHHHHH
LDDLDVVQGVSSVIRLEGTGL
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PGDEKPVGVAVLGLGNVGSEVVRIIENSAEDLAARVGAPLVLRGIGVRRVTTDRGVPIE
CCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHCCCEEEECCCEEEEECCCCCCHH
LLTDDIEELVAREDVDIVVEVMGPVEPSRKAILGALERGKSVVTANKALLATSTGELAQA
HHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCEEEEECCHHHHHH
AESAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIVNGTTNYILSAMDSTGADYAS
HHCCCEEEEEEHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHH
ALADASALGYAEADPTADVEGYDAAAKAAILASIAFHTRVTADDVYREGITKVTPADFGS
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCC
AHALGCTIKLLSICERITTDEGSQRVSARVYPALVPLSHPLAAVNGAFNAVVVEAEAAGR
CHHHHHHHHHHHHHHHHCCCCCCHHHHHEECCEECCCCCCHHHHCCCCEEEEEEECCCCE
LMFYGQGAGGAPTASAVTGDLVMAARNRVLGSRGPRESKYAQLPVAPMGFIETRYYVSMN
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
VADKPGVLSAVAAEFAKREVSIAEVRQEGVVDEGGRRVGARIVVVTHLATDAALSETVDA
CCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEECEEEEEEEEHHHHHHHHHHHHH
LDDLDVVQGVSSVIRLEGTGL
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972