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The map label for this gene is nfdA [H]

Identifier: 148660321

GI number: 148660321

Start: 644199

End: 645803

Strand: Direct

Name: nfdA [H]

Synonym: MRA_0559

Alternate gene names: 148660321

Gene position: 644199-645803 (Clockwise)

Preceding gene: 148660309

Following gene: 148660322

Centisome position: 14.57

GC content: 67.35

Gene sequence:

>1605_bases
ATGGCCGATGCAGACCTCGTCATGACCGGAACCGTGCTCACCGTCGACGATGCGCGGCCAACGGCCGAGGCGATCGCGGT
CGCCGACGGCCGGGTCATTGCCGTCGGTGACCGGTCCGAGGTTGCCGGCCTGGTTGGCGCCAACACCCGGGTCATCGATC
TGGGTGCCGGGTGCGTCATGCCAGGATTTGTTGAGGCACACGGCCATCCGCTACTGGAGGCGGTCGTGCTGTCGGACCGG
TTCGTCGATATCCGTCCGGTGACGATGCGGGACGCGGACGACGTCGTTGCCGCGATCCGCGGCGAGGTTGCACGGCGCGG
CCCGGCCGGCGCCTATCTGGTCGGCTGGGATCCGCTGCTGCAGTCCGGTCTTGGCGAGCCGACGCTGACCTGGCTCGACA
GCCTCGCGCCGAACGGGCCGCTGGTGATCATCCACAACTCCGGACACAAGGCTTACTTCAACTCGCACGCCGCCTGGCTC
AATGGGCTCACCCGAGACACCGCGGATCCCAAGGGCGCGAAGTATGGCCGCGACGGCAATGGCGAACTCGACGGCACCGC
CGAGGAAATCGGCGCGATTCTTCCGCTTTTGGCCGGTGTAGCCGACCCCAGCAACTTCGGTGCCATGCTGCGCGCCGAGT
GTGCTCGGCTCAACCGTGCCGGCCTGACCACATGCTCGGAGATGGCTTTTGACCCAGGGTATCGGCCGATGGTCGAGGCG
GTGCGCGCCGAACTGACGGTCCGGCTGTGCACCTACGAGATCTCCAATGCGCGGATGTGCACCGATGCGACGCCTGGGCA
AGGTGACGACATGCTGCGCCAGGTGGGCATCAAGATCTGGGTGGACGGCTCGCCGTGGGTCGGCAATATCGATCTGACCT
TTCCCTACCTGGACACCCCCGCCACCCGTGCCATCGGTGTACCGCCCGGTTCCCGCGGGTGCGCCAATTACACCCGTGAA
CAGTTGGCCGAAATCGTCGGGGCCTACTTTCCGCGGGGCTGGCAGATCGCCTGTCACGTGCACGGCGACGGCGGTGTGGA
CACCATCCTCGACGTCTACGAAGAGGCACTGCGCCGCAATCCTCGAGACGATCACCGGCTGCGGCTCGAACACGTCGGGG
CCATCCGGCCCGACCAACTGCGGCGCGCCGCCGAACTCGGTGTCACCTGCAGCATCTTCGTCGACCAGATCCATTACTGG
GGCGATGTGATCGTCGATGACCTGTTCGGGGCACAGCGCGGGTCCCGGTGGATGCCGGCTGGATCCGCGGTGGCCGCCGG
CATGCGTATCTCGCTGCACAACGACCCGCCCGTCACACCGGAGGAGCCACTGCGCAACATCAGCGTGGCCGCAACCCGGG
TGGCGCCCAGTGGCCGGGTGCTGGCACCGGAGGAGCGCCTGACGGTCGAGCAGGCGATTCGCGCGCAGACCATCGATGCC
GCCTGGCAACTGTTCGCTGAGGACGCGATCGGCTCGCTTCAGGTCGGCAAGTACGCGGATATGGTGGTGCTGTCGGCGGA
TCCCCGGACGGTGCCGCCAGAGCAGATCGCCGACCTGGCGGTGCGGGCGACGTTTCTGGCCGGTCGCCAGGTTTATCGGC
GGTGA

Upstream 100 bases:

>100_bases
GCATCGTCGCCGGCGGTGCTCATCTGGCAAGACTAGAACGTGTTGCAATTTGGATCTGCCGTGCCCTCGTAATCTCGAAG
GATCACTACGCTTGGAGCCC

Downstream 100 bases:

>100_bases
TACCCGTGCTGCCCCCCCTAGAAGCCCTGCTGGACCGCCTGTATGTGGTGGCCCTGCCGATGCGAGTGCGTTTCCGCGGC
ATCACCACCCGTGAAGTGGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MADADLVMTGTVLTVDDARPTAEAIAVADGRVIAVGDRSEVAGLVGANTRVIDLGAGCVMPGFVEAHGHPLLEAVVLSDR
FVDIRPVTMRDADDVVAAIRGEVARRGPAGAYLVGWDPLLQSGLGEPTLTWLDSLAPNGPLVIIHNSGHKAYFNSHAAWL
NGLTRDTADPKGAKYGRDGNGELDGTAEEIGAILPLLAGVADPSNFGAMLRAECARLNRAGLTTCSEMAFDPGYRPMVEA
VRAELTVRLCTYEISNARMCTDATPGQGDDMLRQVGIKIWVDGSPWVGNIDLTFPYLDTPATRAIGVPPGSRGCANYTRE
QLAEIVGAYFPRGWQIACHVHGDGGVDTILDVYEEALRRNPRDDHRLRLEHVGAIRPDQLRRAAELGVTCSIFVDQIHYW
GDVIVDDLFGAQRGSRWMPAGSAVAAGMRISLHNDPPVTPEEPLRNISVAATRVAPSGRVLAPEERLTVEQAIRAQTIDA
AWQLFAEDAIGSLQVGKYADMVVLSADPRTVPPEQIADLAVRATFLAGRQVYRR

Sequences:

>Translated_534_residues
MADADLVMTGTVLTVDDARPTAEAIAVADGRVIAVGDRSEVAGLVGANTRVIDLGAGCVMPGFVEAHGHPLLEAVVLSDR
FVDIRPVTMRDADDVVAAIRGEVARRGPAGAYLVGWDPLLQSGLGEPTLTWLDSLAPNGPLVIIHNSGHKAYFNSHAAWL
NGLTRDTADPKGAKYGRDGNGELDGTAEEIGAILPLLAGVADPSNFGAMLRAECARLNRAGLTTCSEMAFDPGYRPMVEA
VRAELTVRLCTYEISNARMCTDATPGQGDDMLRQVGIKIWVDGSPWVGNIDLTFPYLDTPATRAIGVPPGSRGCANYTRE
QLAEIVGAYFPRGWQIACHVHGDGGVDTILDVYEEALRRNPRDDHRLRLEHVGAIRPDQLRRAAELGVTCSIFVDQIHYW
GDVIVDDLFGAQRGSRWMPAGSAVAAGMRISLHNDPPVTPEEPLRNISVAATRVAPSGRVLAPEERLTVEQAIRAQTIDA
AWQLFAEDAIGSLQVGKYADMVVLSADPRTVPPEQIADLAVRATFLAGRQVYRR
>Mature_533_residues
ADADLVMTGTVLTVDDARPTAEAIAVADGRVIAVGDRSEVAGLVGANTRVIDLGAGCVMPGFVEAHGHPLLEAVVLSDRF
VDIRPVTMRDADDVVAAIRGEVARRGPAGAYLVGWDPLLQSGLGEPTLTWLDSLAPNGPLVIIHNSGHKAYFNSHAAWLN
GLTRDTADPKGAKYGRDGNGELDGTAEEIGAILPLLAGVADPSNFGAMLRAECARLNRAGLTTCSEMAFDPGYRPMVEAV
RAELTVRLCTYEISNARMCTDATPGQGDDMLRQVGIKIWVDGSPWVGNIDLTFPYLDTPATRAIGVPPGSRGCANYTREQ
LAEIVGAYFPRGWQIACHVHGDGGVDTILDVYEEALRRNPRDDHRLRLEHVGAIRPDQLRRAAELGVTCSIFVDQIHYWG
DVIVDDLFGAQRGSRWMPAGSAVAAGMRISLHNDPPVTPEEPLRNISVAATRVAPSGRVLAPEERLTVEQAIRAQTIDAA
WQLFAEDAIGSLQVGKYADMVVLSADPRTVPPEQIADLAVRATFLAGRQVYRR

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 57341; Mature: 57209

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADADLVMTGTVLTVDDARPTAEAIAVADGRVIAVGDRSEVAGLVGANTRVIDLGAGCVM
CCCCCEEEEEEEEEECCCCCCHHEEEEECCEEEEECCHHHHHHEECCCEEEEEECCCCCC
PGFVEAHGHPLLEAVVLSDRFVDIRPVTMRDADDVVAAIRGEVARRGPAGAYLVGWDPLL
CCHHHHCCCHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECHHHH
QSGLGEPTLTWLDSLAPNGPLVIIHNSGHKAYFNSHAAWLNGLTRDTADPKGAKYGRDGN
HCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCC
GELDGTAEEIGAILPLLAGVADPSNFGAMLRAECARLNRAGLTTCSEMAFDPGYRPMVEA
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHH
VRAELTVRLCTYEISNARMCTDATPGQGDDMLRQVGIKIWVDGSPWVGNIDLTFPYLDTP
HHHHHEEEEEEEEECCCEEECCCCCCCCHHHHHHCCEEEEECCCCCEEEEEEEECCCCCC
ATRAIGVPPGSRGCANYTREQLAEIVGAYFPRGWQIACHVHGDGGVDTILDVYEEALRRN
CCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
PRDDHRLRLEHVGAIRPDQLRRAAELGVTCSIFVDQIHYWGDVIVDDLFGAQRGSRWMPA
CCHHHEEEHHHCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GSAVAAGMRISLHNDPPVTPEEPLRNISVAATRVAPSGRVLAPEERLTVEQAIRAQTIDA
CCHHHCCEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCEECCHHHHHHHHHHHHHHHHH
AWQLFAEDAIGSLQVGKYADMVVLSADPRTVPPEQIADLAVRATFLAGRQVYRR
HHHHHHHHCCCCEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADADLVMTGTVLTVDDARPTAEAIAVADGRVIAVGDRSEVAGLVGANTRVIDLGAGCVM
CCCCEEEEEEEEEECCCCCCHHEEEEECCEEEEECCHHHHHHEECCCEEEEEECCCCCC
PGFVEAHGHPLLEAVVLSDRFVDIRPVTMRDADDVVAAIRGEVARRGPAGAYLVGWDPLL
CCHHHHCCCHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECHHHH
QSGLGEPTLTWLDSLAPNGPLVIIHNSGHKAYFNSHAAWLNGLTRDTADPKGAKYGRDGN
HCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCC
GELDGTAEEIGAILPLLAGVADPSNFGAMLRAECARLNRAGLTTCSEMAFDPGYRPMVEA
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHH
VRAELTVRLCTYEISNARMCTDATPGQGDDMLRQVGIKIWVDGSPWVGNIDLTFPYLDTP
HHHHHEEEEEEEEECCCEEECCCCCCCCHHHHHHCCEEEEECCCCCEEEEEEEECCCCCC
ATRAIGVPPGSRGCANYTREQLAEIVGAYFPRGWQIACHVHGDGGVDTILDVYEEALRRN
CCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
PRDDHRLRLEHVGAIRPDQLRRAAELGVTCSIFVDQIHYWGDVIVDDLFGAQRGSRWMPA
CCHHHEEEHHHCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GSAVAAGMRISLHNDPPVTPEEPLRNISVAATRVAPSGRVLAPEERLTVEQAIRAQTIDA
CCHHHCCEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCEECCHHHHHHHHHHHHHHHHH
AWQLFAEDAIGSLQVGKYADMVVLSADPRTVPPEQIADLAVRATFLAGRQVYRR
HHHHHHHHCCCCEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA