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The map label for this gene is betA [C]

Identifier: 148660259

GI number: 148660259

Start: 582799

End: 584688

Strand: Reverse

Name: betA [C]

Synonym: MRA_0499

Alternate gene names: 148660259

Gene position: 584688-582799 (Counterclockwise)

Preceding gene: 148660260

Following gene: 148660251

Centisome position: 13.23

GC content: 71.9

Gene sequence:

>1890_bases
ATGAGCCGGCTCGCCGATCGGGCTAAGTCCTATCCGTTGGCCTCGTTCGGCGCCGCGCTGCTGCCGCCGGAGCTCGGCGG
CCCGTTGCCGGCTCAATTCGTGCAGCGCGTTGACCGCTACGTCACACGACTGCCGGCGACCTCACGATTCGCGGTGCGCG
CCGGGCTGGCTTCACTGGCGGCGGCCAGCTACCTCACCACCGGCCGGTCGCTACCGCGACTGCACCCGGACGAGCGGGCA
CGGGTGCTGCACCGGATCGCCGCACTGAGCCCGGAAGTCGCTGCGGCCGTGGAGGGGTTGAAGGCGATCGTGCTGTTGGC
CAACGGCGCCGACACCTACGCGCACGAATTGCTCGCCCGCGCCCAGGAACATGACGCGGCGCGCCCCGACGCGGAGTTGA
CCGTCATCTTGTCGGCGGACAGCCCATCCGTCACCAGGGCCGATGCGGTGGTCGTCGGCTCCGGGGCGGGCGGCGCGATG
GTCGCGCGCACGCTGGCCCGGGCCGGCCTTGACGTGGTCGTGCTCGAAGAGGGACGGCGCTGGACGGTCGAGGAGTTCCG
TAGCACCCACCCGGTCGACCGTTATGCCGGGTTGTACCGTGGCGCCGGGGCCACCGTCGCGCTCGGGCGACCGGCGGTGG
TGCTGCCGATGGGCCGGGCGGTGGGCGGCACCACCGTCGTCAACTCCGGCACCTGCTTTCGGCCTTCACTTGCCGTGCAG
CGGCGCTGGCGTGACGAGTTCGGTCTCGGCCTGGCCGACCCCGACCAGCTGGGACGTCGTCTTGACGATGCGGAGCAAAC
ACTGCGGGTTGCGCCGGTGCCGCTGGAGATCATGGGCCGCAACGGGCGCCTGTTGCTGCAGGCCGCCAAGTCGCTGGGCT
GGCGGGCGGCGCCCATCCCGCGCAATGCGCCGGGGTGCCGTGGCTGTTGCCAGTGCGCGATCGGCTGCCCCAGCAACGCC
AAGTTCGGCGTGCACCTCAACGCCCTGCCGCAAGCCTGCGCGGCCGGCGCGCGGATCATCTCCTGGGCGCGGGTCGAACG
GATCCTGCACCGCGCCGGGCGGGCCTATGGCGTGCGAGCCCGCCGGCCCGACGGCACCACACTCGACGTGCTGGCCGACG
CGGTCGTCGTCGCCGCCGGTGCGACGGAGACGCCAGGACTGTTGCGGCGCAGCGGTCTTGGTGGGCACCCGCGACTGGGC
CACAACCTTGCGCTGCACCCGGCAACTATGCTGGCCGGGCTCTTCGACGACGACGTCTTCGCGTGGCGCGGGGTGCTGCA
GAGCGCGGCGGTTCACGAGTTTCACGAATCCGACGGCGTGCTGATCGAGGCCACCTCCACACCGCCGGGCATGGGGTCGA
TGGTCTTCCCCGGCTACGGGGCCGAGCTGCTCCGCTGGCTCGACCGGGCGCCGCAGATCGCAACATTCGGGGCGATGGTG
GCCGATCGGGGCGTCGGCACGGTGCGGTCGGTGCGCGGCGAGACGGTGGTGCGCTATGACATAGCTCCAGGTGAGATCGC
CAAGCTACGGGTCGCGCTGCAGGCCATTGGCCGGCTGCTCTTTGCCGCCGGCGCGGTGGAGGTGCTGACCGGTATCCCCG
GCGCCCCACCGATGCGGTCCCTGCCCGAGCTGCAGGATGTGCTGCGCCGAGCCAACCCCAGAAGCCTGCACCTGGCCGCC
TTCCATCCGACGGGCACCGCGGCCGCCGGCGCCGACGAGCAGCTTTGTCCGGTCGACGCGACGGGCCGGCTGCGCGGCGT
CGAGGGCGTGTGGGTTGCCGACGCCTCGATCCTGCCCAGCTGCCCGGAAGTGAACCCGCAGCTGTCCATCATGGCGATGG
CACTGGCCGTAGCCGACCAGACAGTCGCCAAGGTTGTCGGCGTCCGCTAG

Upstream 100 bases:

>100_bases
ATGTGGCCCGCGCGGAGTGGCCGCTGCACGCGATGGCCGCTGCCATCTTCGCAACGTATCCGTTCTTCATAAAGCTCGGC
CGCCGGCTCGGGCGCCGGAT

Downstream 100 bases:

>100_bases
CCCTCGAGTTTGTAGCCCAGCCCGCGCACCGTCACCAAGTGAACCGGGTTAGCCGGGTCGGCTTCGATCTTGGAGCGCAG
CCGCTTGACATGGACGTCGA

Product: GMC-type oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSRLADRAKSYPLASFGAALLPPELGGPLPAQFVQRVDRYVTRLPATSRFAVRAGLASLAAASYLTTGRSLPRLHPDERA
RVLHRIAALSPEVAAAVEGLKAIVLLANGADTYAHELLARAQEHDAARPDAELTVILSADSPSVTRADAVVVGSGAGGAM
VARTLARAGLDVVVLEEGRRWTVEEFRSTHPVDRYAGLYRGAGATVALGRPAVVLPMGRAVGGTTVVNSGTCFRPSLAVQ
RRWRDEFGLGLADPDQLGRRLDDAEQTLRVAPVPLEIMGRNGRLLLQAAKSLGWRAAPIPRNAPGCRGCCQCAIGCPSNA
KFGVHLNALPQACAAGARIISWARVERILHRAGRAYGVRARRPDGTTLDVLADAVVVAAGATETPGLLRRSGLGGHPRLG
HNLALHPATMLAGLFDDDVFAWRGVLQSAAVHEFHESDGVLIEATSTPPGMGSMVFPGYGAELLRWLDRAPQIATFGAMV
ADRGVGTVRSVRGETVVRYDIAPGEIAKLRVALQAIGRLLFAAGAVEVLTGIPGAPPMRSLPELQDVLRRANPRSLHLAA
FHPTGTAAAGADEQLCPVDATGRLRGVEGVWVADASILPSCPEVNPQLSIMAMALAVADQTVAKVVGVR

Sequences:

>Translated_629_residues
MSRLADRAKSYPLASFGAALLPPELGGPLPAQFVQRVDRYVTRLPATSRFAVRAGLASLAAASYLTTGRSLPRLHPDERA
RVLHRIAALSPEVAAAVEGLKAIVLLANGADTYAHELLARAQEHDAARPDAELTVILSADSPSVTRADAVVVGSGAGGAM
VARTLARAGLDVVVLEEGRRWTVEEFRSTHPVDRYAGLYRGAGATVALGRPAVVLPMGRAVGGTTVVNSGTCFRPSLAVQ
RRWRDEFGLGLADPDQLGRRLDDAEQTLRVAPVPLEIMGRNGRLLLQAAKSLGWRAAPIPRNAPGCRGCCQCAIGCPSNA
KFGVHLNALPQACAAGARIISWARVERILHRAGRAYGVRARRPDGTTLDVLADAVVVAAGATETPGLLRRSGLGGHPRLG
HNLALHPATMLAGLFDDDVFAWRGVLQSAAVHEFHESDGVLIEATSTPPGMGSMVFPGYGAELLRWLDRAPQIATFGAMV
ADRGVGTVRSVRGETVVRYDIAPGEIAKLRVALQAIGRLLFAAGAVEVLTGIPGAPPMRSLPELQDVLRRANPRSLHLAA
FHPTGTAAAGADEQLCPVDATGRLRGVEGVWVADASILPSCPEVNPQLSIMAMALAVADQTVAKVVGVR
>Mature_628_residues
SRLADRAKSYPLASFGAALLPPELGGPLPAQFVQRVDRYVTRLPATSRFAVRAGLASLAAASYLTTGRSLPRLHPDERAR
VLHRIAALSPEVAAAVEGLKAIVLLANGADTYAHELLARAQEHDAARPDAELTVILSADSPSVTRADAVVVGSGAGGAMV
ARTLARAGLDVVVLEEGRRWTVEEFRSTHPVDRYAGLYRGAGATVALGRPAVVLPMGRAVGGTTVVNSGTCFRPSLAVQR
RWRDEFGLGLADPDQLGRRLDDAEQTLRVAPVPLEIMGRNGRLLLQAAKSLGWRAAPIPRNAPGCRGCCQCAIGCPSNAK
FGVHLNALPQACAAGARIISWARVERILHRAGRAYGVRARRPDGTTLDVLADAVVVAAGATETPGLLRRSGLGGHPRLGH
NLALHPATMLAGLFDDDVFAWRGVLQSAAVHEFHESDGVLIEATSTPPGMGSMVFPGYGAELLRWLDRAPQIATFGAMVA
DRGVGTVRSVRGETVVRYDIAPGEIAKLRVALQAIGRLLFAAGAVEVLTGIPGAPPMRSLPELQDVLRRANPRSLHLAAF
HPTGTAAAGADEQLCPVDATGRLRGVEGVWVADASILPSCPEVNPQLSIMAMALAVADQTVAKVVGVR

Specific function: Can Catalyze The Oxidation Of Choline To Betaine Aldehyde And Betaine Aldehyde To Glycine Betaine At The Same Rate. [C]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y492_MYCTU (Q11157)

Other databases:

- EMBL:   BX842573
- EMBL:   AE000516
- EMBL:   AE000516
- PIR:   G70744
- RefSeq:   NP_215006.2
- RefSeq:   NP_334920.1
- RefSeq:   NP_334921.1
- ProteinModelPortal:   Q11157
- EnsemblBacteria:   EBMYCT00000000947
- EnsemblBacteria:   EBMYCT00000084082
- GeneID:   887199
- GeneID:   923878
- GeneID:   923879
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv0492c
- TIGR:   MT0511
- TIGR:   MT0512
- TubercuList:   Rv0492c
- GeneTree:   EBGT00050000015738
- HOGENOM:   HBG569846
- ProtClustDB:   CLSK799713
- GO:   GO:0005829
- InterPro:   IPR000172
- InterPro:   IPR007867

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 66155; Mature: 66024

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00623 GMC_OXRED_1; PS00624 GMC_OXRED_2; PS00198 4FE4S_FERREDOXIN ; PS00211 ABC_TRANSPORTER_1

Important sites: ACT_SITE 562-562

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLADRAKSYPLASFGAALLPPELGGPLPAQFVQRVDRYVTRLPATSRFAVRAGLASLA
CCHHHHHHHCCCCHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AASYLTTGRSLPRLHPDERARVLHRIAALSPEVAAAVEGLKAIVLLANGADTYAHELLAR
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCEEEEEEECCCHHHHHHHHHH
AQEHDAARPDAELTVILSADSPSVTRADAVVVGSGAGGAMVARTLARAGLDVVVLEEGRR
HHHHCCCCCCCEEEEEEECCCCCCEECCEEEEECCCCHHHHHHHHHHCCCEEEEECCCCE
WTVEEFRSTHPVDRYAGLYRGAGATVALGRPAVVLPMGRAVGGTTVVNSGTCFRPSLAVQ
ECHHHHHCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCEEECCCCCCCCHHHHH
RRWRDEFGLGLADPDQLGRRLDDAEQTLRVAPVPLEIMGRNGRLLLQAAKSLGWRAAPIP
HHHHHHHCCCCCCHHHHHHHHCHHHHHHEECCCCCEEECCCCHHHHHHHHHCCCCCCCCC
RNAPGCRGCCQCAIGCPSNAKFGVHLNALPQACAAGARIISWARVERILHRAGRAYGVRA
CCCCCCHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RRPDGTTLDVLADAVVVAAGATETPGLLRRSGLGGHPRLGHNLALHPATMLAGLFDDDVF
CCCCCCCHHHHHHHHHEECCCCCCCHHHHHCCCCCCCCCCCCEEECHHHHHHHHCCCHHH
AWRGVLQSAAVHEFHESDGVLIEATSTPPGMGSMVFPGYGAELLRWLDRAPQIATFGAMV
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
ADRGVGTVRSVRGETVVRYDIAPGEIAKLRVALQAIGRLLFAAGAVEVLTGIPGAPPMRS
HHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LPELQDVLRRANPRSLHLAAFHPTGTAAAGADEQLCPVDATGRLRGVEGVWVADASILPS
CHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
CPEVNPQLSIMAMALAVADQTVAKVVGVR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRLADRAKSYPLASFGAALLPPELGGPLPAQFVQRVDRYVTRLPATSRFAVRAGLASLA
CHHHHHHHCCCCHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AASYLTTGRSLPRLHPDERARVLHRIAALSPEVAAAVEGLKAIVLLANGADTYAHELLAR
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCEEEEEEECCCHHHHHHHHHH
AQEHDAARPDAELTVILSADSPSVTRADAVVVGSGAGGAMVARTLARAGLDVVVLEEGRR
HHHHCCCCCCCEEEEEEECCCCCCEECCEEEEECCCCHHHHHHHHHHCCCEEEEECCCCE
WTVEEFRSTHPVDRYAGLYRGAGATVALGRPAVVLPMGRAVGGTTVVNSGTCFRPSLAVQ
ECHHHHHCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCEEECCCCCCCCHHHHH
RRWRDEFGLGLADPDQLGRRLDDAEQTLRVAPVPLEIMGRNGRLLLQAAKSLGWRAAPIP
HHHHHHHCCCCCCHHHHHHHHCHHHHHHEECCCCCEEECCCCHHHHHHHHHCCCCCCCCC
RNAPGCRGCCQCAIGCPSNAKFGVHLNALPQACAAGARIISWARVERILHRAGRAYGVRA
CCCCCCHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RRPDGTTLDVLADAVVVAAGATETPGLLRRSGLGGHPRLGHNLALHPATMLAGLFDDDVF
CCCCCCCHHHHHHHHHEECCCCCCCHHHHHCCCCCCCCCCCCEEECHHHHHHHHCCCHHH
AWRGVLQSAAVHEFHESDGVLIEATSTPPGMGSMVFPGYGAELLRWLDRAPQIATFGAMV
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
ADRGVGTVRSVRGETVVRYDIAPGEIAKLRVALQAIGRLLFAAGAVEVLTGIPGAPPMRS
HHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LPELQDVLRRANPRSLHLAAFHPTGTAAAGADEQLCPVDATGRLRGVEGVWVADASILPS
CHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
CPEVNPQLSIMAMALAVADQTVAKVVGVR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036