Click here to switch to the map view.

The map label for this gene is fgd1

Identifier: 148660172

GI number: 148660172

Start: 492093

End: 493103

Strand: Direct

Name: fgd1

Synonym: MRA_0413

Alternate gene names: 148660172

Gene position: 492093-493103 (Clockwise)

Preceding gene: 148660170

Following gene: 148660173

Centisome position: 11.13

GC content: 64.09

Gene sequence:

>1011_bases
GTGGCTGAACTGAAGCTAGGTTACAAAGCATCGGCCGAACAATTCGCACCGCGCGAGCTCGTCGAACTAGCCGTCGCCGC
CGAAGCCCACGGCATGGACAGCGCGACCGTCAGCGACCATTTTCAGCCTTGGCGCCACCAGGGCGGCCATGCCCCGTTCT
CGCTGTCCTGGATGACCGCTGTCGGCGAACGTACCAACCGGCTGCTGCTGGGCACTTCGGTGCTGACCCCCACCTTCCGC
TACAACCCCGCCGTCATCGCTCAGGCTTTCGCCACCATGGGATGCCTGTACCCGAACCGTGTTTTCCTTGGCGTGGGCAC
CGGTGAGGCGCTGAACGAAATCGCCACCGGATACGAGGGCGCCTGGCCGGAGTTCAAGGAGCGGTTCGCCCGGCTGCGTG
AATCGGTGGGGCTAATGCGGCAGCTGTGGAGCGGTGACCGCGTCGACTTTGACGGCGACTATTACCGGCTCAAGGGTGCC
TCGATCTACGACGTGCCCGACGGGGGCGTGCCCGTCTACATCGCCGCCGGCGGCCCGGCGGTGGCCAAGTACGCCGGCCG
CGCCGGTGACGGCTTCATCTGTACGTCCGGCAAGGGCGAGGAGCTCTACACCGAGAAGCTGATGCCGGCGGTACGAGAAG
GCGCCGCTGCCGCTGACCGATCCGTCGACGGCATCGACAAGATGATCGAAATCAAGATCTCCTACGACCCCGACCCGGAG
CTGGCATTGAACAACACCCGGTTTTGGGCGCCGCTGTCGTTGACAGCTGAGCAGAAGCACAGCATCGACGACCCGATCGA
GATGGAGAAGGCCGCCGATGCGCTGCCAATCGAACAGATCGCCAAGCGCTGGATCGTGGCGTCGGACCCCGACGAAGCCG
TCGAAAAGGTAGGTCAATACGTGACATGGGGCCTGAACCACCTGGTATTTCACGCACCAGGACATGACCAGCGCCGGTTT
CTGGAGCTCTTCCAGTCGGACCTGGCACCCAGGTTGCGGCGACTTGGCTGA

Upstream 100 bases:

>100_bases
CGGGTGCCGCGGGTCGCGACCATGGGATATGGAGCGATCGCGAGCGCGGCGAAGCCGGGCGTGGCGGGTCGCGTTTATGG
CATAGGAGTAGAAAGAACTG

Downstream 100 bases:

>100_bases
CTCCTCGGCGATCTACCTCGCCGCACCAGAATCGCAGACGGGTAAGTCGACGATTGCACTGGGGCTTTTGCACCGACTGA
CCGCGATGGTCGCCAAAGTC

Product: putative f420-dependent glucose-6-phosphate dehydrogenase Fgd1

Products: NA

Alternate protein names: F420_G6P_DH

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MAELKLGYKASAEQFAPRELVELAVAAEAHGMDSATVSDHFQPWRHQGGHAPFSLSWMTAVGERTNRLLLGTSVLTPTFR
YNPAVIAQAFATMGCLYPNRVFLGVGTGEALNEIATGYEGAWPEFKERFARLRESVGLMRQLWSGDRVDFDGDYYRLKGA
SIYDVPDGGVPVYIAAGGPAVAKYAGRAGDGFICTSGKGEELYTEKLMPAVREGAAAADRSVDGIDKMIEIKISYDPDPE
LALNNTRFWAPLSLTAEQKHSIDDPIEMEKAADALPIEQIAKRWIVASDPDEAVEKVGQYVTWGLNHLVFHAPGHDQRRF
LELFQSDLAPRLRRLG

Sequences:

>Translated_336_residues
MAELKLGYKASAEQFAPRELVELAVAAEAHGMDSATVSDHFQPWRHQGGHAPFSLSWMTAVGERTNRLLLGTSVLTPTFR
YNPAVIAQAFATMGCLYPNRVFLGVGTGEALNEIATGYEGAWPEFKERFARLRESVGLMRQLWSGDRVDFDGDYYRLKGA
SIYDVPDGGVPVYIAAGGPAVAKYAGRAGDGFICTSGKGEELYTEKLMPAVREGAAAADRSVDGIDKMIEIKISYDPDPE
LALNNTRFWAPLSLTAEQKHSIDDPIEMEKAADALPIEQIAKRWIVASDPDEAVEKVGQYVTWGLNHLVFHAPGHDQRRF
LELFQSDLAPRLRRLG
>Mature_335_residues
AELKLGYKASAEQFAPRELVELAVAAEAHGMDSATVSDHFQPWRHQGGHAPFSLSWMTAVGERTNRLLLGTSVLTPTFRY
NPAVIAQAFATMGCLYPNRVFLGVGTGEALNEIATGYEGAWPEFKERFARLRESVGLMRQLWSGDRVDFDGDYYRLKGAS
IYDVPDGGVPVYIAAGGPAVAKYAGRAGDGFICTSGKGEELYTEKLMPAVREGAAAADRSVDGIDKMIEIKISYDPDPEL
ALNNTRFWAPLSLTAEQKHSIDDPIEMEKAADALPIEQIAKRWIVASDPDEAVEKVGQYVTWGLNHLVFHAPGHDQRRFL
ELFQSDLAPRLRRLG

Specific function: Involved in the bioreductive activation of the prodrug PA-824 (nitroimidazo-oxazine) developed for anti-tuberculosis therapy against both replicating and persistent bacteria. It does not interact directly with PA-824 but, rather, provides reduced F420 to

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the F420-dependent glucose-6-phosphate dehydrogenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FGD1_MYCTU (P96253)

Other databases:

- EMBL:   BX842573
- EMBL:   AE000516
- PIR:   E70628
- RefSeq:   NP_214921.1
- RefSeq:   NP_334830.1
- PDB:   3B4Y
- PDB:   3C8N
- PDBsum:   3B4Y
- PDBsum:   3C8N
- ProteinModelPortal:   P96253
- SMR:   P96253
- EnsemblBacteria:   EBMYCT00000001611
- EnsemblBacteria:   EBMYCT00000069765
- GeneID:   886418
- GeneID:   923683
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0420
- KEGG:   mtu:Rv0407
- TIGR:   MT0420
- TubercuList:   Rv0407
- GeneTree:   EBGT00070000031894
- HOGENOM:   HBG564988
- OMA:   VDFEGEY
- ProtClustDB:   CLSK871799
- BioCyc:   MetaCyc:MONOMER-15448
- InterPro:   IPR019945
- InterPro:   IPR019944
- InterPro:   IPR011251
- InterPro:   IPR016048
- Gene3D:   G3DSA:3.20.20.30
- TIGRFAMs:   TIGR03554
- TIGRFAMs:   TIGR03557

Pfam domain/function: PF00296 Bac_luciferase; SSF51679 Luciferase_like

EC number: =1.1.99.34

Molecular weight: Translated: 36989; Mature: 36858

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00683 RHODANESE_2

Important sites: BINDING 39-39 BINDING 76-76 BINDING 112-112 BINDING 195-195 BINDING 198-198 BINDING 283-283

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKLGYKASAEQFAPRELVELAVAAEAHGMDSATVSDHFQPWRHQGGHAPFSLSWMTA
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCCCCCCEEEHHHHH
VGERTNRLLLGTSVLTPTFRYNPAVIAQAFATMGCLYPNRVFLGVGTGEALNEIATGYEG
HHHCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCC
AWPEFKERFARLRESVGLMRQLWSGDRVDFDGDYYRLKGASIYDVPDGGVPVYIAAGGPA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEECCCCCEEEEEECCCHH
VAKYAGRAGDGFICTSGKGEELYTEKLMPAVREGAAAADRSVDGIDKMIEIKISYDPDPE
HHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHCCCHHCCCEEEEEEEEECCCCC
LALNNTRFWAPLSLTAEQKHSIDDPIEMEKAADALPIEQIAKRWIVASDPDEAVEKVGQY
EEECCCEEEEEEECCCHHHCCCCCCHHHHHHHCCCCHHHHHHHCEECCCHHHHHHHHHHH
VTWGLNHLVFHAPGHDQRRFLELFQSDLAPRLRRLG
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AELKLGYKASAEQFAPRELVELAVAAEAHGMDSATVSDHFQPWRHQGGHAPFSLSWMTA
CCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCCCCCCEEEHHHHH
VGERTNRLLLGTSVLTPTFRYNPAVIAQAFATMGCLYPNRVFLGVGTGEALNEIATGYEG
HHHCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCC
AWPEFKERFARLRESVGLMRQLWSGDRVDFDGDYYRLKGASIYDVPDGGVPVYIAAGGPA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCEEEECCCCCEEEEEECCCHH
VAKYAGRAGDGFICTSGKGEELYTEKLMPAVREGAAAADRSVDGIDKMIEIKISYDPDPE
HHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHCCCHHCCCEEEEEEEEECCCCC
LALNNTRFWAPLSLTAEQKHSIDDPIEMEKAADALPIEQIAKRWIVASDPDEAVEKVGQY
EEECCCEEEEEEECCCHHHCCCCCCHHHHHHHCCCCHHHHHHHCEECCCHHHHHHHHHHH
VTWGLNHLVFHAPGHDQRRFLELFQSDLAPRLRRLG
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036