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The map label for this gene is lipW [H]

Identifier: 148659981

GI number: 148659981

Start: 261285

End: 262193

Strand: Reverse

Name: lipW [H]

Synonym: MRA_0225

Alternate gene names: 148659981

Gene position: 262193-261285 (Counterclockwise)

Preceding gene: 148659987

Following gene: 148659979

Centisome position: 5.93

GC content: 67.22

Gene sequence:

>909_bases
GTGTCCGGCAACGAAGTCCACCCCGACCTGCGTCGCATCGCCGTCGTCACCCCACGACAGCTGGTCGGTCCTCGCACCCT
GCCAGTCATGCGGGCATTGATCGTCGTCGCGGGGCTTCGAATGTCCCGTACACCCCCCGATATCGAGGTGCTCACCCTGG
AATCCGGGGTCGGTGTCCGGCTATACCGACCCGCCGGCAGCAACGAACCAGCGCCCGCGCTGCTGTGGATCCACGCCGGC
GGATACGTAATGGGCACCGCGCAACAGGACGATCGGCTCTGCCTCCGGTTCAGCAGCAGACTGGGCATCACTGTCGCATC
GGTGGACTACCGCCTGGCGCCGGAAAATCCGTATCCTGCCGCCCTGGGCGACTGCTACTCGGCGTTGACCTGGCTGGCCA
GCCTGCCGGCGGTGGACCCCGCGCGGGTGGCAATCGGCGGCGCTAGTGCCGGCGGCGGCCTCGCGGCGGCGCTGGCTCTG
CTTGCCCGCGACCGTGGCGGCATCACACCGGCATTTCAGTTGCTGGTGTACCCCATGCTCGACGACCGGCCGTCGATCGC
CCCCGCCAACCCGCACTACCGGCTATGGAACGGGCGCGCCAACCGGTTCGGCTGGCGGGCATATCTTGGCGATGCGGACG
CACGGGTCGCGGTCCCGGGACGGCGTGACGACCTAGGAGGGCTGGCGCCGGCATGGATCGGCGTCGGCACCCACGACCTG
CTCCACGACGAGGATCTGGCCTATGCCGAACGTCTGACAGCAGCCGGGGTGCCATGCCAGGTAGAAGTAGTCGAAGGCGC
CTTCCACGGTTTCGATCGTGTGGCCCCGAATGTTGGGGTCTCACAAAGGTTCTTCACTAGTCAGTGCAACAGTTTGCGTG
CCGCGCTGGCCCTTAGTAACCGAACCTAG

Upstream 100 bases:

>100_bases
TCACTCATCAGGCCACCATCCCGATCCTCGTTCGACTAACCGCAGCCTGCCAAAGCCATCGCTGTACCGAGGCGAAATTG
CGCCGACGCATACTGGACCG

Downstream 100 bases:

>100_bases
AATTGCAAGGCGCTAAAACGCCAGTCGAGCACCTGCCGATCGGGCTCACTCGCCGAATCGCTGACCGCGTAGACCAGTGA
CCGCAGTCCCAGCACACCCC

Product: putative esterase LipW

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSGNEVHPDLRRIAVVTPRQLVGPRTLPVMRALIVVAGLRMSRTPPDIEVLTLESGVGVRLYRPAGSNEPAPALLWIHAG
GYVMGTAQQDDRLCLRFSSRLGITVASVDYRLAPENPYPAALGDCYSALTWLASLPAVDPARVAIGGASAGGGLAAALAL
LARDRGGITPAFQLLVYPMLDDRPSIAPANPHYRLWNGRANRFGWRAYLGDADARVAVPGRRDDLGGLAPAWIGVGTHDL
LHDEDLAYAERLTAAGVPCQVEVVEGAFHGFDRVAPNVGVSQRFFTSQCNSLRAALALSNRT

Sequences:

>Translated_302_residues
MSGNEVHPDLRRIAVVTPRQLVGPRTLPVMRALIVVAGLRMSRTPPDIEVLTLESGVGVRLYRPAGSNEPAPALLWIHAG
GYVMGTAQQDDRLCLRFSSRLGITVASVDYRLAPENPYPAALGDCYSALTWLASLPAVDPARVAIGGASAGGGLAAALAL
LARDRGGITPAFQLLVYPMLDDRPSIAPANPHYRLWNGRANRFGWRAYLGDADARVAVPGRRDDLGGLAPAWIGVGTHDL
LHDEDLAYAERLTAAGVPCQVEVVEGAFHGFDRVAPNVGVSQRFFTSQCNSLRAALALSNRT
>Mature_301_residues
SGNEVHPDLRRIAVVTPRQLVGPRTLPVMRALIVVAGLRMSRTPPDIEVLTLESGVGVRLYRPAGSNEPAPALLWIHAGG
YVMGTAQQDDRLCLRFSSRLGITVASVDYRLAPENPYPAALGDCYSALTWLASLPAVDPARVAIGGASAGGGLAAALALL
ARDRGGITPAFQLLVYPMLDDRPSIAPANPHYRLWNGRANRFGWRAYLGDADARVAVPGRRDDLGGLAPAWIGVGTHDLL
HDEDLAYAERLTAAGVPCQVEVVEGAFHGFDRVAPNVGVSQRFFTSQCNSLRAALALSNRT

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=319, Percent_Identity=26.9592476489028, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI206597554, Length=284, Percent_Identity=26.056338028169, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI157041239, Length=300, Percent_Identity=27, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI68051721, Length=167, Percent_Identity=29.3413173652695, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1786682, Length=256, Percent_Identity=26.953125, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI72001146, Length=134, Percent_Identity=38.0597014925373, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567059, Length=144, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71996133, Length=158, Percent_Identity=29.746835443038, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=145, Percent_Identity=32.4137931034483, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 32217; Mature: 32086

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNEVHPDLRRIAVVTPRQLVGPRTLPVMRALIVVAGLRMSRTPPDIEVLTLESGVGVR
CCCCCCCCHHHEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEE
LYRPAGSNEPAPALLWIHAGGYVMGTAQQDDRLCLRFSSRLGITVASVDYRLAPENPYPA
EECCCCCCCCCCEEEEEEECCEEEECCCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCH
ALGDCYSALTWLASLPAVDPARVAIGGASAGGGLAAALALLARDRGGITPAFQLLVYPML
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHEEECCC
DDRPSIAPANPHYRLWNGRANRFGWRAYLGDADARVAVPGRRDDLGGLAPAWIGVGTHDL
CCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCHHHHCCCCHHH
LHDEDLAYAERLTAAGVPCQVEVVEGAFHGFDRVAPNVGVSQRFFTSQCNSLRAALALSN
HCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEECC
RT
CC
>Mature Secondary Structure 
SGNEVHPDLRRIAVVTPRQLVGPRTLPVMRALIVVAGLRMSRTPPDIEVLTLESGVGVR
CCCCCCCHHHEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEE
LYRPAGSNEPAPALLWIHAGGYVMGTAQQDDRLCLRFSSRLGITVASVDYRLAPENPYPA
EECCCCCCCCCCEEEEEEECCEEEECCCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCH
ALGDCYSALTWLASLPAVDPARVAIGGASAGGGLAAALALLARDRGGITPAFQLLVYPML
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHEEECCC
DDRPSIAPANPHYRLWNGRANRFGWRAYLGDADARVAVPGRRDDLGGLAPAWIGVGTHDL
CCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCHHHHCCCCHHH
LHDEDLAYAERLTAAGVPCQVEVVEGAFHGFDRVAPNVGVSQRFFTSQCNSLRAALALSN
HCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEECC
RT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]