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The map label for this gene is nadR [H]

Identifier: 148659976

GI number: 148659976

Start: 255031

End: 256002

Strand: Reverse

Name: nadR [H]

Synonym: MRA_0220

Alternate gene names: 148659976

Gene position: 256002-255031 (Counterclockwise)

Preceding gene: 148659977

Following gene: 148659972

Centisome position: 5.79

GC content: 65.64

Gene sequence:

>972_bases
GTGACACACGGAATGGTGCTCGGCAAGTTCATGCCGCCCCATGCGGGACACGTCTACCTTTGCGAGTTCGCGCGGCGATG
GGTGGATGAGCTGACCATCGTCGTCGGATCAACGGCAGCAGAGCCGATTCCGGGCGCCCAGCGCGTTGCATGGATGCGGG
AGCTGTTCCCCTTCGATCGCGTGGTCCATCTGGCCAACGAGAACCCGCAGCGCCCGTGGGAGCACCCGGACTTCTGGGAC
ATCTGGAAGGCGAGCCTGCAGGGCGTGCTGGCAACCCGCCCCGACTTCGTCTTCGGTGCCGAGCCCTACAACGCGGACTT
TGCCCAGGTCCTCGGAGCGCGTTTCGTGGCGGTCGATCACGGTCGCACCGTCGTTCCCGTGACTGCAACCGACATCCGCG
CGGACCCGCTTGGCCACTGGCAACACATCCCACGGTGCGTGCGGCCGGCCTTCGTCAAACGCGTGAGCATCATCGGACCC
GAATCCACCGGGAAGACCACGCTGGCACAGGCGGTTGCGGAAAAGCTCCGAACGAAGTGGGTCCCGGAGCGGGCGAAAAT
GTTGCGGGAGCTCAATGGCGGCTCACTGATAGGACTGGAGTGGGCCGAAATCGTTCGCGGACAGATCGCGTCGGAGGAAG
CCTTGGCTCGTGACGCCGATCGCGTCCTGATCTGCGACACGGATCCGCTCGCGACGACCGTGTGGGCCGAGTTCCTGGCG
GGCGGCTGCCCGCAAGAGCTCCGTGATCTAGCTCGGCGTCCCTACGATCTCACACTGCTCACCACGCCCGATGTGCCCTG
GGACGCCGACGACGGACGCTGTGTCCCCGGCGCACGCGGTACCTTTTTCGCCCGCTGCGAGCAGGCTCTCCGCGCCGCGG
GACGATCATTCGTGGTGATCACGGGCGGTTGGGAAGAGAGGCTTTCGGTGTCTTTGCGCGCTGTCGAAGAACTTGTGCGT
GCCCGCCGCTAA

Upstream 100 bases:

>100_bases
CTGACGTCGGAATCAGCTTCGATGAGGCTGAGGCGTACTTTCTTTCGCGGCTGGACGAGTGGGAGAATGTCATCGCCGGC
CGAATCGCTGAGGCGACGTC

Downstream 100 bases:

>100_bases
CGAGCGCGAGCAGACACTAAAGCCCTCGACACGCCGTGCGTGCGGGGGCTTATGCGTCTGCTCGCCCTAACCTAGGCGCT
CCTTCAGGGCGTCGAACTCA

Product: putative transcriptional regulatory protein NadR

Products: NA

Alternate protein names: Transcriptional regulator NadR; Nicotinamide mononucleotide adenylyltransferase; NMN adenylyltransferase; NMN-AT; NMNAT; Nicotinamide ribonucleotide adenylyltransferase; Nicotinamide-nucleotide adenylyltransferase; Ribosylnicotinamide kinase; RNK; Nicotinamide riboside kinase; NRK; NmR-K [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTHGMVLGKFMPPHAGHVYLCEFARRWVDELTIVVGSTAAEPIPGAQRVAWMRELFPFDRVVHLANENPQRPWEHPDFWD
IWKASLQGVLATRPDFVFGAEPYNADFAQVLGARFVAVDHGRTVVPVTATDIRADPLGHWQHIPRCVRPAFVKRVSIIGP
ESTGKTTLAQAVAEKLRTKWVPERAKMLRELNGGSLIGLEWAEIVRGQIASEEALARDADRVLICDTDPLATTVWAEFLA
GGCPQELRDLARRPYDLTLLTTPDVPWDADDGRCVPGARGTFFARCEQALRAAGRSFVVITGGWEERLSVSLRAVEELVR
ARR

Sequences:

>Translated_323_residues
MTHGMVLGKFMPPHAGHVYLCEFARRWVDELTIVVGSTAAEPIPGAQRVAWMRELFPFDRVVHLANENPQRPWEHPDFWD
IWKASLQGVLATRPDFVFGAEPYNADFAQVLGARFVAVDHGRTVVPVTATDIRADPLGHWQHIPRCVRPAFVKRVSIIGP
ESTGKTTLAQAVAEKLRTKWVPERAKMLRELNGGSLIGLEWAEIVRGQIASEEALARDADRVLICDTDPLATTVWAEFLA
GGCPQELRDLARRPYDLTLLTTPDVPWDADDGRCVPGARGTFFARCEQALRAAGRSFVVITGGWEERLSVSLRAVEELVR
ARR
>Mature_322_residues
THGMVLGKFMPPHAGHVYLCEFARRWVDELTIVVGSTAAEPIPGAQRVAWMRELFPFDRVVHLANENPQRPWEHPDFWDI
WKASLQGVLATRPDFVFGAEPYNADFAQVLGARFVAVDHGRTVVPVTATDIRADPLGHWQHIPRCVRPAFVKRVSIIGPE
STGKTTLAQAVAEKLRTKWVPERAKMLRELNGGSLIGLEWAEIVRGQIASEEALARDADRVLICDTDPLATTVWAEFLAG
GCPQELRDLARRPYDLTLLTTPDVPWDADDGRCVPGARGTFFARCEQALRAAGRSFVVITGGWEERLSVSLRAVEELVRA
RR

Specific function: This enzyme has three activities:DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase

COG id: COG3172

COG function: function code H; Predicted ATPase/kinase involved in NAD metabolism

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein. Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082440, Length=347, Percent_Identity=30.5475504322767, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016429
- InterPro:   IPR004821
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR006417
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: =2.7.7.1; =2.7.1.22 [H]

Molecular weight: Translated: 36032; Mature: 35900

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHGMVLGKFMPPHAGHVYLCEFARRWVDELTIVVGSTAAEPIPGAQRVAWMRELFPFDR
CCCCEEEECCCCCCCCEEHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHCCHHH
VVHLANENPQRPWEHPDFWDIWKASLQGVLATRPDFVFGAEPYNADFAQVLGARFVAVDH
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCEEEEEEC
GRTVVPVTATDIRADPLGHWQHIPRCVRPAFVKRVSIIGPESTGKTTLAQAVAEKLRTKW
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCC
VPERAKMLRELNGGSLIGLEWAEIVRGQIASEEALARDADRVLICDTDPLATTVWAEFLA
CCHHHHHHHHCCCCCEEECHHHHHHHHHHCHHHHHHCCCCEEEEECCCCHHHHHHHHHHC
GGCPQELRDLARRPYDLTLLTTPDVPWDADDGRCVPGARGTFFARCEQALRAAGRSFVVI
CCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEE
TGGWEERLSVSLRAVEELVRARR
ECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THGMVLGKFMPPHAGHVYLCEFARRWVDELTIVVGSTAAEPIPGAQRVAWMRELFPFDR
CCCEEEECCCCCCCCEEHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHCCHHH
VVHLANENPQRPWEHPDFWDIWKASLQGVLATRPDFVFGAEPYNADFAQVLGARFVAVDH
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCEEEEEEC
GRTVVPVTATDIRADPLGHWQHIPRCVRPAFVKRVSIIGPESTGKTTLAQAVAEKLRTKW
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCC
VPERAKMLRELNGGSLIGLEWAEIVRGQIASEEALARDADRVLICDTDPLATTVWAEFLA
CCHHHHHHHHCCCCCEEECHHHHHHHHHHCHHHHHHCCCCEEEEECCCCHHHHHHHHHHC
GGCPQELRDLARRPYDLTLLTTPDVPWDADDGRCVPGARGTFFARCEQALRAAGRSFVVI
CCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEE
TGGWEERLSVSLRAVEELVRARR
ECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 1327967 [H]