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The map label for this gene is trmB

Identifier: 148659972

GI number: 148659972

Start: 249477

End: 250268

Strand: Reverse

Name: trmB

Synonym: MRA_0216

Alternate gene names: 148659972

Gene position: 250268-249477 (Counterclockwise)

Preceding gene: 148659976

Following gene: 148659971

Centisome position: 5.66

GC content: 64.77

Gene sequence:

>792_bases
ATGGTCCACCATGGACAAATGCATGCGCAACCCGGGGTAGGGCTACGTCCCGACACACCGGTGGCATCGGGCCAGCTCCC
CAGCACGAGCATCCGGTCGCGGCGGTCGGGGATCTCCAAGGCCCAACGCGAGACCTGGGAGCGACTTTGGCCGGAGCTTG
GCCTCCTGGCGCTACCTCAATCCCCGCGCGGGACACCGGTGGATACCCGTGCCTGGTTTGGCCGTGACGCGCCGGTGGTG
TTGGAGATCGGCTCCGGCAGCGGCACGTCGACGCTGGCGATGGCGAAGGCCGAACCCCATGTCGACGTGATCGCTGTGGA
CGTCTATCGCCGCGGGCTGGCTCAGCTGCTCTGCGCGATTGACAAAGTGGGCTCCGACGGGATCAACATCCGGCTGATCC
TTGGCAATGCCGTCGACGTGCTGCAACACTTGATCGCCCCCGATTCGTTGTGCGGTGTTCGAGTCTTCTTCCCCGATCCG
TGGCCGAAGGCACGCCACCATAAGCGCCGCCTGTTACAGCCGGCCACCATGGCCTTGATCGCAGACCGGCTCGTTCCCAG
TGGTGTGCTGCACGCCGCGACCGACCATCCCGGCTACGCCGAGCACATCGCCGCGGCCGGCGACGCCGAACCCCGGCTGG
TTCGCGTCGACCCCGACACCGAACTGCTGCCGATTTCGGTCGTCCGACCGGCTACCAAGTACGAGAGGAAAGCCCAGCTT
GGGGGCGGCGCAGTCATCGAGTTGTTATGGAAAAAGCACGGTTGCTCTGAAAGAGACCTGAAAATTAGATGA

Upstream 100 bases:

>100_bases
GCACCCTCGCCGCCGAGGCGCGAGTCAGCAGTTGTCCGCTACCCGCCGAAGGCAACTGTTGGGTATCAATCTGGACCAGG
GGCGGCTACGCCGGGCGCTG

Downstream 100 bases:

>100_bases
TGATGAGCCTGACCGAAGACGTGACTTCACAAACATCTGAATCCCTCGCCCGGCACTCGGTACTGGCGGAAGACTTATCG
CAGGACGGGCTGACCAGTCT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MVHHGQMHAQPGVGLRPDTPVASGQLPSTSIRSRRSGISKAQRETWERLWPELGLLALPQSPRGTPVDTRAWFGRDAPVV
LEIGSGSGTSTLAMAKAEPHVDVIAVDVYRRGLAQLLCAIDKVGSDGINIRLILGNAVDVLQHLIAPDSLCGVRVFFPDP
WPKARHHKRRLLQPATMALIADRLVPSGVLHAATDHPGYAEHIAAAGDAEPRLVRVDPDTELLPISVVRPATKYERKAQL
GGGAVIELLWKKHGCSERDLKIR

Sequences:

>Translated_263_residues
MVHHGQMHAQPGVGLRPDTPVASGQLPSTSIRSRRSGISKAQRETWERLWPELGLLALPQSPRGTPVDTRAWFGRDAPVV
LEIGSGSGTSTLAMAKAEPHVDVIAVDVYRRGLAQLLCAIDKVGSDGINIRLILGNAVDVLQHLIAPDSLCGVRVFFPDP
WPKARHHKRRLLQPATMALIADRLVPSGVLHAATDHPGYAEHIAAAGDAEPRLVRVDPDTELLPISVVRPATKYERKAQL
GGGAVIELLWKKHGCSERDLKIR
>Mature_263_residues
MVHHGQMHAQPGVGLRPDTPVASGQLPSTSIRSRRSGISKAQRETWERLWPELGLLALPQSPRGTPVDTRAWFGRDAPVV
LEIGSGSGTSTLAMAKAEPHVDVIAVDVYRRGLAQLLCAIDKVGSDGINIRLILGNAVDVLQHLIAPDSLCGVRVFFPDP
WPKARHHKRRLLQPATMALIADRLVPSGVLHAATDHPGYAEHIAAAGDAEPRLVRVDPDTELLPISVVRPATKYERKAQL
GGGAVIELLWKKHGCSERDLKIR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=229, Percent_Identity=36.2445414847162, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_MYCBO (P67499)

Other databases:

- EMBL:   BX248334
- RefSeq:   NP_853879.1
- ProteinModelPortal:   P67499
- SMR:   P67499
- EnsemblBacteria:   EBMYCT00000017011
- GeneID:   1090931
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0214c
- GeneTree:   EBGT00050000017066
- HOGENOM:   HBG322782
- OMA:   NIRLIRG
- ProtClustDB:   PRK00121
- BioCyc:   MBOV233413:MB0214C-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 28547; Mature: 28547

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: BINDING 82-82 BINDING 107-107 BINDING 136-136 BINDING 159-159 BINDING 163-163 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHHGQMHAQPGVGLRPDTPVASGQLPSTSIRSRRSGISKAQRETWERLWPELGLLALPQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC
SPRGTPVDTRAWFGRDAPVVLEIGSGSGTSTLAMAKAEPHVDVIAVDVYRRGLAQLLCAI
CCCCCCCCCHHHCCCCCCEEEEECCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHH
DKVGSDGINIRLILGNAVDVLQHLIAPDSLCGVRVFFPDPWPKARHHKRRLLQPATMALI
HHCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHH
ADRLVPSGVLHAATDHPGYAEHIAAAGDAEPRLVRVDPDTELLPISVVRPATKYERKAQL
HHHHCCCCCEECCCCCCCHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHC
GGGAVIELLWKKHGCSERDLKIR
CCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVHHGQMHAQPGVGLRPDTPVASGQLPSTSIRSRRSGISKAQRETWERLWPELGLLALPQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC
SPRGTPVDTRAWFGRDAPVVLEIGSGSGTSTLAMAKAEPHVDVIAVDVYRRGLAQLLCAI
CCCCCCCCCHHHCCCCCCEEEEECCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHH
DKVGSDGINIRLILGNAVDVLQHLIAPDSLCGVRVFFPDPWPKARHHKRRLLQPATMALI
HHCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHH
ADRLVPSGVLHAATDHPGYAEHIAAAGDAEPRLVRVDPDTELLPISVVRPATKYERKAQL
HHHHCCCCCEECCCCCCCHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHC
GGGAVIELLWKKHGCSERDLKIR
CCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972