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The map label for this gene is pntB [H]

Identifier: 148659921

GI number: 148659921

Start: 186412

End: 187839

Strand: Direct

Name: pntB [H]

Synonym: MRA_0165

Alternate gene names: 148659921

Gene position: 186412-187839 (Clockwise)

Preceding gene: 148659920

Following gene: 148659922

Centisome position: 4.22

GC content: 64.43

Gene sequence:

>1428_bases
ATGAACCTGCACTACCTGGTCGAGATTCTCTACATCATCTCCTTTTCACTCTTCATCTACGGGTTGATGGGGCTCACCGG
CCCCAAGACCGCGGTGCGCGGGAACCTGATCGCCGCGGCCGGCATGACCATCGCCGTGGCGGCCACGTTGGTCATGATCC
GACACACCAGCCAATGGCCGCTGATCATCGCCGGTCTGGTGGTGGGTGTTGTGCTCGGTGTGCCGCCGGCGCGACTGACC
AAGATGACCGCCATGCCGCAGCTGGTGGCATTCTTCAACGGCGTGGGCGGAGGAACGGTCGCACTCATCGCGCTGTCGGA
GTTCATCGATACCACCGGCTTTTCCGCATTCCAGCACGGCGAGTCGCCGACCGTGCACATCGTGGTGGCCTCATTGTTCG
CCGCGATCATCGGGTCGATCTCGTTCTGGGGGTCTATCGTCGCGTTCGGCAAGTTGCAGGAGATCATCTCCGGGCGGCCG
ATCGGACTCGGCAAGGCGCAGCAGCCGATCAACCTGTTGCTGCTGGCCGTGGCCGTGGCCGCCGCCGTGGTGATCGGACT
GCACGCGCATCCCGGGAGCGGTGGGGTCGCATTGTGGTGGATGATCGGCCTGTTGGTCGCCGCCGGCGTGCTGGGTCTGA
TGGTGGTGTTGCCGATCGGTGGCGCCGACATGCCGGTGGTCATCTCGATGCTCAACGCCATGACCGGCCTGTCGGCCGCG
GCGGCGGGTCTGGCGTTGAACAACACCGCGATGATCGTGGCCGGCATGATCGTCGGCGCGTCCGGCTCGATCCTGACCAA
CCTGATGGCTAAGGCGATGAACCGCTCCATTCCGGCGATCGTCGCGGGCGGTTTCGGCGGCGGCGGTGTGGCGCCCAGTG
GCGGCGGCGACGACAAACACGTCAAGGCCACTTCGGCCGCCGATGCCGCGATCCAGATGGCATACGCCAATCAGGTGATC
GTGGTGCCCGGCTACGGGTTGGCCGTCGCGCAGGCGCAGCATGCGGTGAAGGACCTGGCAACCTTGCTGGAGGACAGGGG
TGTGCCGGTCAAGTACGCGATTCACCCGGTCGCCGGCCGGATGCCCGGGCATATGAACGTGCTGCTGGCCGAGGCCGAAG
TCGACTACGACGCGATGAAGGACATGGACGACATCAACGACGAGTTCGCCCGCACCGACGTCACCATCGTGATCGGCGCC
AACGACGTCACCAACCCGGCGGCCCGCAACGAGACGTCCAGCCCGATCTACGGCATGCCGATCCTCAACGTGGACAAGTC
GAGGTCGGTGATCGTGCTCAAACGGTCGATGAATTCCGGGTTCGCCGGCATCGACAACCCGCTGTTCTACGCCGACGGCA
CCACTATGTTGTTCGGTGATGCGAAGAAATCGGTGACCGAAGTCTCCGAGGAACTCAAGGCGTTGTAG

Upstream 100 bases:

>100_bases
ACGTCATCGGCGGATTCATCGTCACCGACCGAATGCTCGGCATGTTCAAGGCCAAGAAGCCCGCCGTGCCAGCCAAGCCC
GACCGCGACGAGGCGCTCCG

Downstream 100 bases:

>100_bases
CGCGCGAGCGCTGGCTCAGACGGGCGGATACGCCGGCGGCGGGTATCCGTCGCCGGTTTCGACCCCGCGTAGACCCCAGG
TGAGGTACCGGAAGAAGAAC

Product: NAD(P) transhydrogenase subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNLHYLVEILYIISFSLFIYGLMGLTGPKTAVRGNLIAAAGMTIAVAATLVMIRHTSQWPLIIAGLVVGVVLGVPPARLT
KMTAMPQLVAFFNGVGGGTVALIALSEFIDTTGFSAFQHGESPTVHIVVASLFAAIIGSISFWGSIVAFGKLQEIISGRP
IGLGKAQQPINLLLLAVAVAAAVVIGLHAHPGSGGVALWWMIGLLVAAGVLGLMVVLPIGGADMPVVISMLNAMTGLSAA
AAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIPAIVAGGFGGGGVAPSGGGDDKHVKATSAADAAIQMAYANQVI
VVPGYGLAVAQAQHAVKDLATLLEDRGVPVKYAIHPVAGRMPGHMNVLLAEAEVDYDAMKDMDDINDEFARTDVTIVIGA
NDVTNPAARNETSSPIYGMPILNVDKSRSVIVLKRSMNSGFAGIDNPLFYADGTTMLFGDAKKSVTEVSEELKAL

Sequences:

>Translated_475_residues
MNLHYLVEILYIISFSLFIYGLMGLTGPKTAVRGNLIAAAGMTIAVAATLVMIRHTSQWPLIIAGLVVGVVLGVPPARLT
KMTAMPQLVAFFNGVGGGTVALIALSEFIDTTGFSAFQHGESPTVHIVVASLFAAIIGSISFWGSIVAFGKLQEIISGRP
IGLGKAQQPINLLLLAVAVAAAVVIGLHAHPGSGGVALWWMIGLLVAAGVLGLMVVLPIGGADMPVVISMLNAMTGLSAA
AAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIPAIVAGGFGGGGVAPSGGGDDKHVKATSAADAAIQMAYANQVI
VVPGYGLAVAQAQHAVKDLATLLEDRGVPVKYAIHPVAGRMPGHMNVLLAEAEVDYDAMKDMDDINDEFARTDVTIVIGA
NDVTNPAARNETSSPIYGMPILNVDKSRSVIVLKRSMNSGFAGIDNPLFYADGTTMLFGDAKKSVTEVSEELKAL
>Mature_475_residues
MNLHYLVEILYIISFSLFIYGLMGLTGPKTAVRGNLIAAAGMTIAVAATLVMIRHTSQWPLIIAGLVVGVVLGVPPARLT
KMTAMPQLVAFFNGVGGGTVALIALSEFIDTTGFSAFQHGESPTVHIVVASLFAAIIGSISFWGSIVAFGKLQEIISGRP
IGLGKAQQPINLLLLAVAVAAAVVIGLHAHPGSGGVALWWMIGLLVAAGVLGLMVVLPIGGADMPVVISMLNAMTGLSAA
AAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIPAIVAGGFGGGGVAPSGGGDDKHVKATSAADAAIQMAYANQVI
VVPGYGLAVAQAQHAVKDLATLLEDRGVPVKYAIHPVAGRMPGHMNVLLAEAEVDYDAMKDMDDINDEFARTDVTIVIGA
NDVTNPAARNETSSPIYGMPILNVDKSRSVIVLKRSMNSGFAGIDNPLFYADGTTMLFGDAKKSVTEVSEELKAL

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=472, Percent_Identity=37.9237288135593, Blast_Score=289, Evalue=5e-78,
Organism=Homo sapiens, GI122939153, Length=472, Percent_Identity=37.9237288135593, Blast_Score=289, Evalue=5e-78,
Organism=Escherichia coli, GI1787886, Length=478, Percent_Identity=44.9790794979079, Blast_Score=355, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI17550456, Length=469, Percent_Identity=38.592750533049, Blast_Score=298, Evalue=4e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 48859; Mature: 48859

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHYLVEILYIISFSLFIYGLMGLTGPKTAVRGNLIAAAGMTIAVAATLVMIRHTSQWP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEECCCHHHHHHHHHHHHHCCCCCH
LIIAGLVVGVVLGVPPARLTKMTAMPQLVAFFNGVGGGTVALIALSEFIDTTGFSAFQHG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHCC
ESPTVHIVVASLFAAIIGSISFWGSIVAFGKLQEIISGRPIGLGKAQQPINLLLLAVAVA
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AAVVIGLHAHPGSGGVALWWMIGLLVAAGVLGLMVVLPIGGADMPVVISMLNAMTGLSAA
HHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIPAIVAGGFGGGGVAPSGGGDDKH
HHCCEECCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCE
VKATSAADAAIQMAYANQVIVVPGYGLAVAQAQHAVKDLATLLEDRGVPVKYAIHPVAGR
EEECCHHHHHHEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
MPGHMNVLLAEAEVDYDAMKDMDDINDEFARTDVTIVIGANDVTNPAARNETSSPIYGMP
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEECCE
ILNVDKSRSVIVLKRSMNSGFAGIDNPLFYADGTTMLFGDAKKSVTEVSEELKAL
EEECCCCCCEEEEEECCCCCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLHYLVEILYIISFSLFIYGLMGLTGPKTAVRGNLIAAAGMTIAVAATLVMIRHTSQWP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEECCCHHHHHHHHHHHHHCCCCCH
LIIAGLVVGVVLGVPPARLTKMTAMPQLVAFFNGVGGGTVALIALSEFIDTTGFSAFQHG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHCC
ESPTVHIVVASLFAAIIGSISFWGSIVAFGKLQEIISGRPIGLGKAQQPINLLLLAVAVA
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AAVVIGLHAHPGSGGVALWWMIGLLVAAGVLGLMVVLPIGGADMPVVISMLNAMTGLSAA
HHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIPAIVAGGFGGGGVAPSGGGDDKH
HHCCEECCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCE
VKATSAADAAIQMAYANQVIVVPGYGLAVAQAQHAVKDLATLLEDRGVPVKYAIHPVAGR
EEECCHHHHHHEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
MPGHMNVLLAEAEVDYDAMKDMDDINDEFARTDVTIVIGANDVTNPAARNETSSPIYGMP
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEECCE
ILNVDKSRSVIVLKRSMNSGFAGIDNPLFYADGTTMLFGDAKKSVTEVSEELKAL
EEECCCCCCEEEEEECCCCCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]