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The map label for this gene is hycD [H]

Identifier: 148659846

GI number: 148659846

Start: 93686

End: 94636

Strand: Direct

Name: hycD [H]

Synonym: MRA_0088

Alternate gene names: 148659846

Gene position: 93686-94636 (Clockwise)

Preceding gene: 148659845

Following gene: 148659847

Centisome position: 2.12

GC content: 68.56

Gene sequence:

>951_bases
ATGTCCTACCTAGCGGGCGCCGCGCAAATCGGCGGGGTCATGGTGGGTGCGCCGCTGGTCATCGGTATGACGCGGCAGGT
ACGGGCACGCTGGGAAGGCCGGGCCGGCGCCGGCCTGCTGCAACCGTGGCGTGATCTGCTCAAACAGCTTGGCAAGCAAC
AGATCACACCGGCGGGGACGACGATCGTGTTCGCCGCCGCGCCGGTGATCGTCGCCGGGACAACGCTTTTGATCGCCGCG
ATCGCACCTCTGGTGGCCACCGGGTCACCCCTGGACCCCAGCGCCGACTTGTTTGCCGTGGTCGGGCTGCTATTCCTGGG
CACCGTCGCACTGACCCTGGCCGGCATCGACACCGGCACCTCTTTCGGCGGCATGGGCGCCAGCCGCGAGATCACCATCG
CCGCACTGGTCGAACCAACGATCCTGCTGGCGGTGTTCGCGCTGTCCATCCCCGCCGGATCGGCCAATCTCGGTGCGCTG
GTGGCGAGTACGATCGACCACCCGGGCCACGTGGTGTCGCTGGCCGGCGTACTGGCCTTCGTGGCGTTGGTGATTGTCAT
CGTCGCCGAGACCGGGCGGCTGCCGGTGGACAACCCGGCCACCCACCTGGAATTGACGATGGTGCACGAGGCCATGGTCC
TCGAGTACGCCGGCCCACGGCTGGCGCTGGTCGAATGGGCGGCCGGGATGCGGCTCACGGTGCTGCTGGCACTGCTGGCG
AATCTGTTCCTGCCGTGGGGGATCGCCGGCGCCGCGCCCACCGCGCTCGACGTGTTGACCGGCGTGGTGGCGGTGGCGGC
CAAGGTCGCGATTCTCGCGGTGCTGCTGGCGACGTTCGAGGTGTTCCTCGCCAAACTGCGATTGTTCCGGGTACCCGAAC
TGCTGGCCGGCTCGTTTCTGCTGGCCTTGCTCGCGGTCACCGCCGCCAACTTCTTCACGGTGGGGGCGTGA

Upstream 100 bases:

>100_bases
CCGAGCTGCTGCGCCGTGCCCACACCGGCAGCGTGCACCGCTACCTGGCCTACGGCGCGCTGGGCGTACTGATCGTGCTG
GTGGTCGCGAGGTGAACGTG

Downstream 100 bases:

>100_bases
GGGGCCAGCGATGAGTAACGCCAACTTCTCGATCCTGGTCGACTTCGCCGCGGGTGGGCTGGTGTTGGCGTCGGTGCTGA
TTGTCTGGCGCCGCGACCTG

Product: formate hydrogenlyase HycD

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSYLAGAAQIGGVMVGAPLVIGMTRQVRARWEGRAGAGLLQPWRDLLKQLGKQQITPAGTTIVFAAAPVIVAGTTLLIAA
IAPLVATGSPLDPSADLFAVVGLLFLGTVALTLAGIDTGTSFGGMGASREITIAALVEPTILLAVFALSIPAGSANLGAL
VASTIDHPGHVVSLAGVLAFVALVIVIVAETGRLPVDNPATHLELTMVHEAMVLEYAGPRLALVEWAAGMRLTVLLALLA
NLFLPWGIAGAAPTALDVLTGVVAVAAKVAILAVLLATFEVFLAKLRLFRVPELLAGSFLLALLAVTAANFFTVGA

Sequences:

>Translated_316_residues
MSYLAGAAQIGGVMVGAPLVIGMTRQVRARWEGRAGAGLLQPWRDLLKQLGKQQITPAGTTIVFAAAPVIVAGTTLLIAA
IAPLVATGSPLDPSADLFAVVGLLFLGTVALTLAGIDTGTSFGGMGASREITIAALVEPTILLAVFALSIPAGSANLGAL
VASTIDHPGHVVSLAGVLAFVALVIVIVAETGRLPVDNPATHLELTMVHEAMVLEYAGPRLALVEWAAGMRLTVLLALLA
NLFLPWGIAGAAPTALDVLTGVVAVAAKVAILAVLLATFEVFLAKLRLFRVPELLAGSFLLALLAVTAANFFTVGA
>Mature_315_residues
SYLAGAAQIGGVMVGAPLVIGMTRQVRARWEGRAGAGLLQPWRDLLKQLGKQQITPAGTTIVFAAAPVIVAGTTLLIAAI
APLVATGSPLDPSADLFAVVGLLFLGTVALTLAGIDTGTSFGGMGASREITIAALVEPTILLAVFALSIPAGSANLGALV
ASTIDHPGHVVSLAGVLAFVALVIVIVAETGRLPVDNPATHLELTMVHEAMVLEYAGPRLALVEWAAGMRLTVLLALLAN
LFLPWGIAGAAPTALDVLTGVVAVAAKVAILAVLLATFEVFLAKLRLFRVPELLAGSFLLALLAVTAANFFTVGA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=292, Percent_Identity=33.9041095890411, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87082115, Length=232, Percent_Identity=35.7758620689655, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 32154; Mature: 32023

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLAGAAQIGGVMVGAPLVIGMTRQVRARWEGRAGAGLLQPWRDLLKQLGKQQITPAGT
CCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHCCCCCCC
TIVFAAAPVIVAGTTLLIAAIAPLVATGSPLDPSADLFAVVGLLFLGTVALTLAGIDTGT
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SFGGMGASREITIAALVEPTILLAVFALSIPAGSANLGALVASTIDHPGHVVSLAGVLAF
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
VALVIVIVAETGRLPVDNPATHLELTMVHEAMVLEYAGPRLALVEWAAGMRLTVLLALLA
HHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHH
NLFLPWGIAGAAPTALDVLTGVVAVAAKVAILAVLLATFEVFLAKLRLFRVPELLAGSFL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALLAVTAANFFTVGA
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYLAGAAQIGGVMVGAPLVIGMTRQVRARWEGRAGAGLLQPWRDLLKQLGKQQITPAGT
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHCCCCCCC
TIVFAAAPVIVAGTTLLIAAIAPLVATGSPLDPSADLFAVVGLLFLGTVALTLAGIDTGT
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SFGGMGASREITIAALVEPTILLAVFALSIPAGSANLGALVASTIDHPGHVVSLAGVLAF
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
VALVIVIVAETGRLPVDNPATHLELTMVHEAMVLEYAGPRLALVEWAAGMRLTVLLALLA
HHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHHH
NLFLPWGIAGAAPTALDVLTGVVAVAAKVAILAVLLATFEVFLAKLRLFRVPELLAGSFL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALLAVTAANFFTVGA
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]